Abstract : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
The potential role of fungal organisms and their co-aggregation with either periodontopathogens or opportunistic pathogens at peri-implantitis sites is unknown. The aim of the present study was to qualitatively/quantitatively analyze and correlate fungal organisms and bacterial species at peri-implantitis sites.
In a total of 29 patients, submucosal/subgingival plaque samples were collected at peri-implantitis and healthy implant sites as well as teeth with a history of periodontitis (controls). A real-time PCR assay was established for the qualification of fungal organisms and a TaqMan assay for the quantification of Porphyromonas gingivalis, Parvimonas micra, Tannerella forsythia, Mycoplasma salivarium, Veillonella parvula, and Staphylococcus aureus.
Fungal organisms were more frequently identified at peri-implantitis (31.6%) (i.e., Candida albicans, Candida boidinii, Penicillium spp., Rhodotorula laryngis, Paelicomyces spp., Saccharomycetes, Cladosporium cladosporioides) and healthy implant sites (40% - Candida dubliniensis, C. cladosporioides) than at selected teeth (20% - C. albicans, Fusarium solani). At implant sites, fungal organisms were significantly correlated with P. micra and T. forsythia.
Candida spp. and other fungal organisms were frequently identified at peri-implantitis as well as healthy implant sites and co-colonized with P. micra and T. forsythia.
Serial posts:
- Abstract : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Background : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Methods : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [1]
- Methods : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [2]
- Methods : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [3]
- Results : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [1]
- Results : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [2]
- Discussion : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [1]
- Discussion : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [2]
- Conclusions : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Abbreviations : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- References : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [1]
- References : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [2]
- References : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites [3]
- Acknowledgements : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Author information : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Additional information : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Rights and permissions : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- About this article : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Table 1 Overview of bacterial species, corresponding genes, primers/probes, and DNA sequences : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Table 2 Overview of fungal organisms, corresponding genes, primers/probes, and DNA sequences : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Table 3 Bacterial and fungal analysis: peri-implantitis sites (n = 19 patients) : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Table 4 Bacterial and fungal analysis: healthy implant sites (n = 10 patients) : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites
- Table 5 Bacterial and fungal analysis: tooth sites (n = 10 patients) : Real-time PCR analysis of fungal organisms and bacterial species at peri-implantitis sites