Materials and methods : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
The research protocol was submitted to and approved by the Ethical Committee of the University of Medical Sciences, School of Dentistry, Havana, Cuba (prot. 013/2013).
Eight female Pelibuey sheep, with a mean body weight of approximately 35 kg and a mean age of approximately 3 years, were provided by the Centro Nacional para la Producción de Animales de Laboratorio (CENPALAB) in Havana, Cuba. The surgical sessions were performed at the Centro de Cirugía Experimental (CENCEX) Facultad de Medicina “Victoria de Girón”, Universidad de Ciencias Médicas in Havana, Cuba. The animals were kept at the facilities of CENPALAB during the experimental period.
The ARRIVE checklist was followed as a guide. According to the Three R requirements [20], aiming to reduce the number of animals, the interferences among animals was eliminated, adopting a split-mouth design. No previous experiments were available that studied healing at the repositioned bony plate, so to describe the event of healing with sufficient approximation, a relevant difference in bone formation within the sinus was set to 10%, assuming a standard deviation of approximately 8%. Considering these values clinically relevant, seven pairs of subjects were calculated to be able to reject the null hypothesis that this response difference is zero with a power of 0.8 and α = 0.05.
Two different procedures for antrostomy closure were adopted, one using a resorbable membrane (control site) and the other repositioning the bone plate (experimental site). The randomisation was performed electronically (www.randomization.com) by an author not involved in any surgical procedures (DB). The surgeon (AP) was informed of the treatment randomly selected only at the end of the grafting procedure.
The examiner of the histological slides (AP) was carefully trained before the evaluation. The measurements were performed twice, and mean values used.
The animals fasted for 24 h preoperatively, but were allowed to drink water ad libitum. Anaesthesia was induced by 0.4 mg/kg of midazolam (Dormicum; Roche, Basel, Switzerland) and 10 mg/kg of ketamine (Ketamina-50; Liorad, Havana, Cuba), and orotracheal intubation was performed. The anaesthesia was maintained with a mixture of oxygen and 2–3% isoflurane (Isoflurane-vet; Merial, Toulouse, France) at a rate of 5 L/min. The surgical sites were rinsed with 0.12% chlorhexidine digluconate (Periogard™; Colgate-Palmolive Ltd, New York, NY, USA) and trichotomy was performed. After general anaesthesia, 1.5–2 cc of 2% mepivacaine HCI with 1:100,000 epinephrine was injected at the surgery site. All surgeries were performed under sterile conditions, using good clinical and laboratory practices.
Serial posts:
- Abstract : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Introduction : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- Introduction : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
- Materials and methods : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- Materials and methods : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
- Materials and methods : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [3]
- Materials and methods : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [4]
- Results : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- Results : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
- Discussion : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- Discussion : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
- Discussion : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [3]
- Conclusion : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Availability of data and materials : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Abbreviations : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- References : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- References : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
- References : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [3]
- References : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [4]
- Acknowledgements : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Funding : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Author information : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [1]
- Author information : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep [2]
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- About this article : Bone plate repositioned over the antrostomy after sinus floor elevation: an experimental study in sheep
- Table 1 Percentages of the various tissues within the elevated area after 4 months of healing. Mean values ± standard deviations (P values) and median (25%; 75% percentiles) : Bone plate repositioned
- Table 2 Percentages of various tissues in the antrostomy area after 4 months of healing. Mean values ± standard deviations (P values) and median (25%; 75% percentiles) : Bone plate repositioned over
- Fig. 1. Clinical view at a membrane site. a Skin and periosteum were separately elevated, and the facial sinus wall exposed. b A 12 × 8-mm window was cut and removed. c The Schneiderian membrane was carefully elevated. d A twisted wire was inserted in the middle of the long side of the window and the elevated sinus was grafted. e At the control site, a resorbable membrane was placed and secured with cyanoacrylate. f Membrane in situ : Bone plate repositioned over the antrostomy after
- Fig. 2. Clinical view at a bone plate site. a The bone window was removed. b The sinus mucosa was carefully elevated, and a twisted wire was placed. c The elevated sinus was grafted. d The access bony window was repositioned and secured with cyanoacrylate : Bone plate repositioned over the antrostomy after
- Fig. 3. a The elevated area was divided into four regions for morphometric analysis. RED: submucosa; GREEN: middle; YELLOW: base; PURPLE: close-to-window. INC: top of the infraorbital nerve canal : Bone plate repositioned over the antrostomy after
- Fig. 4. Photomicrographs of ground sections after 4 months of healing. a Bone formed from the base of the sinus. b Bone plate connected by bridges of the new bone to the close-to-window region. c Particle of the graft surrounded by new bone. d Overexposed image to show the new bone ingrowth within the granules of biomaterial : Bone plate repositioned over the antrostomy after
- Fig. 5. Graph representing the tissue percentages within the elevated area. No statistically significant differences were found : Bone plate repositioned over the antrostomy after
- Fig. 6. Graph representing new bone and composite bone percentages within the elevated area : Bone plate repositioned over the antrostomy after