Materials and methods : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [3]
Overlapping calibrated digital images of the tissues were recorded with Leica Applications Suite version 4.4.0 software from a bright field Leica DM4000 B microscope (Leica Microsystems GmbH, Wëtzlar, Germany) equipped with a 5× lens and DFC420 digital camera. Single images were pasted and merged to compose each elevated sinus using the program Photoshop (Adobe Photoshop CC 2015.0.0).
The histomorphometric measurements were taken by an expert assessor (JM) using a lattice with squares of 75 μm. The following different areas were assessed within the grafted regions (Fig. 2): (i) close to the bone walls (Bone walls region), (ii) center of the augmented region (middle region), (iii) subjacent to the sinus mucosa (sub-mucosa region), (iv) and close to the antrostomy (close-to-window region). Moreover, the antrostomy (antrostomy region) was also evaluated in three different areas: adjacent to the medial and lateral edges, and the middle of the access window (Fig. 2). The tissues evaluated were newly formed bone, graft material, and soft tissues. Percentages were subsequently calculated.
The sites that received autogenous bone in the antrostomy and the subjacent regions (close-to-window) were considered as a test (treated sites) while the sites that did not receive the autogenous bone were considered as control (untreated sites).
The primary variable was the new bone percentage in the region of major interest that was the antrostomy and close-to-window areas. The mean new bone within the augmented sinus was considered as a secondary variable. Mean values and standard deviations were calculated for each outcome variable. The results were calculated using the software Excel 2013 (Microsoft Corporation, Redmond, WA, USA), while the statistical analyses were performed with the IBM SPSS Statistics software v.19 (IBM Inc., Chicago, IL, USA). In the text are reported only mean values and standard deviations while the tables were complemented with 25th, 50th (median), and 75th percentiles were also added. The Wilcoxon test was used to evaluate differences between the two sites with a level of significance set at 5%.
Serial posts:
- Abstract : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- Introduction : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [1]
- Introduction : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [2]
- Materials and methods : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [1]
- Materials and methods : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [2]
- Materials and methods : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [3]
- Results : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [1]
- Results : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [2]
- Discussion : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [1]
- Discussion : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [2]
- Discussion : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [3]
- Availability of data and materials : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- Abbreviations : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- References : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [1]
- References : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [2]
- References : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [3]
- References : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits [4]
- Funding : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
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- About this article : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- Table 1 Histomorphometric analysis. Tissues evaluated in the various regions after 1 week of healing : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- Table 2 Histomorphometric analysis. Tissues evaluated in the various regions after 8 weeks of healing : Influence of the use of autogenous bone particles to close the access window after maxillary sinus floor augmentation: an experimental study in rabbits
- Fig. 1. Clinical view of the surgical procedures. a Tibial bone exposed for autogenous bone harvesting using a bone scraper. b Antrostomies prepared. c Autogenous bone particles placed in the antrostomy. d Xenograft and bone particles (red arrow) at the antrostomies. e Collagen membranes placed on the antrostomies. f Wounds closed with sutures : Influence of the use of autogenous bone particles
- Fig. 2. The various regions evaluated at the histomorphometric analyses. Bone walls (red arrow); middle (white arrow); sub-mucosa (yellow arrow); close-to-window (orange arrow). The antrostomy region was also evaluated at the medial and lateral edges (dark green arrows) and in the middle aspect (light green arrow) : Influence of the use of autogenous bone particles
- Fig. 3. Photomicrographs of decalcified sections illustrating the healing after 1 week. a Treated site. Bone strips occupying the antrostomy and the subjacent area (close-to-window region). b Untreated site. Note the new bone-forming from the sinus bone walls. Scarlet-acid fuchsine and toluidine blue stain. Images grabbed at × 20 magnification : Influence of the use of autogenous bone particles
- Fig. 4. Photomicrographs of ground sections. a) Treated site. Bone residues (examples in yellow asterisks) included in soft tissue containing fibroblast-like cells and inflammatory cells. b) Untreated site. Xenograft residues (examples in red asterisks) surrounded by soft tissue rich in fibroblast-like cells. Scarlet-acid fuchsine and toluidine blue stain. a) 200 x magnification.; b) 100 x magnification : Influence of the use of autogenous bone particles
- Fig. 5. Photomicrographs of decalcified sections illustrating the healing after 8 weeks. Both at the treated (a) and untreated (b) sites, the antrostomy was closed in most cases, presenting residual defects of various dimensions in the outer side. New bone was connecting the lateral and medial sinus walls. The middle and sub-mucosa regions were not healed completely yet. Scarlet-acid fuchsine and toluidine blue stain. Images grabbed at × 20 magnification : Influence of the use of autogenous bone particles