hPDSCs may be a suitable resource of cells for maxillofacial and alveolar bone regeneration.
Conclusions: Porous collagen-hydroxyapatite scaffolds ...
author: Li Ning, DDS, PhDHans Malmstrm, DDSYan-Fang Ren, DDS, MPH, PhD | publisher: drg. Andreas Tjandra, Sp. Perio, FISID
Conclusions
The porous Col-HA composites developed in the present study are biocompatible and can be used as scaffolds for bone tissue regeneration. The Col-HA ratio is an important factor in promoting the attachment and proliferation of mouse MSCs. The Col-HA composite complexes have strong potentials in bone tissue regeneration applications. hPDSCs may be a suitable resource of cells for maxillofacial and alveolar bone regeneration.
Abbreviations
Col |
collagen |
DMEM |
Dulbecco's Modified Eagle Medium |
HA |
hydroxyapatite |
hPDSCs |
human periodontal stem cells |
MSCs |
mesenchymal stem cells |
SEM |
scanning electron microscope |
Acknowledgments
This study was supported by American Academy of Implant Dentistry (AAID) Research.
References
- Janicki P, Boeuf S, Steck E, Egermann M, Kasten P, Richter W. Prediction of in vivo bone forming potency of bone marrow-derived human mesenchymal stem cells. Eur Cell Mater. 2011;21:488–507.
- Prescott RS, Alsanea R, Fayad MI, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen Scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–426.
- Hutmacher DW, Garcia AJ. Scaffold-based bone engineering by using genetically modified cells. Gene. 2005;347:1–10.
- Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002;84-A:454–464.
- Norton MR, Odell EW, Thompson ID, Cook RJ. Efficacy of bovine bone mineral for alveolar augmentation: a human histologic study. Clin Oral Implants Res. 2003;14:775–783.
- Hoexter DL. Bone regeneration graft materials. J Oral Implant. 2002;28:290–294.
- Miranda SC, Silva GA, Mendes RM, et al. Mesenchymal stem cells associated with porous chitosan-gelatin scaffold: a potential strategy for alveolar bone regeneration. J Biomed Mater Res Part A. 2012;100:2775–2786.
- El-Ghannam A. Bone reconstruction: from bioceramics to tissue engineering. Expert Rev Med Devices. 2005;2:87–101.
- Henderson JA, He X, Jabbari E. Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 2008;8:499–507.
- Bose S, Roy M, Bandyopadhyay A. Recent advances in bone tissue engineering scaffolds. Trends Biotechnol. 2012;30:546–554.
- Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT. Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg. 2008;90(suppl 1):36–42.
- Yunoki S, Ikoma T, Tsuchiya A, et al. Fabrication and mechanical and tissue ingrowth properties of unidirectionally porous hydroxyapatite/collagen composite. J Biomed Mater Res Part B Appl Biomater. 2007;80B:166–173.
- Wahl DA, Czernuszka JT. Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater. 2006;11:43–56.
- Zhang SM, Cui FZ, Liao SS, Zhu Y, Han L. Synthesis and biocompatibility of porous nano-hydroxyapatite/collagen/alginate composite. J Mater Sci Mater Med. 2003;14:641–645.
- Zhu X, Eibl O, Scheideler L, Geis-Gerstorfer J. Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors. J Biomed Mater Res Part A. 2006;79:114–127.
- Chan CK, Kumar TS, Liao S, Murugan R, Ngiam M, Ramakrishnan S. Biomimetic nanocomposites for bone graft applications. Nanomedicine (Lond). 2006;1:177–188.
- Detsch R, Uhl F, Deisinger U, Ziegler G. 3D-Cultivation of bone marrow stromal cells on hydroxyapatite scaffolds fabricated by dispense-plotting and negative mould technique. J Mater Sci Mater Med. 2008;19:1491–1496.
- Liao S, Wang W, Uo M, et al. A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials. 2005;26:7564–7571.
- Kikuchi M, Matsumoto HN, Yamada T, Koyama Y, Takakuda K, Tanaka J. Glutaraldehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites. Biomaterials. 2004;25:63–69.
- Geiger M. Porous Collagen/Ceramic Composite Carriers for Bone Regeneration Using Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) [dissertation]. Erlangen-Nuremberg, Germany: University of Erlangen-Nuremberg; 2001.
- Wang H, Li Y, Zuo Y, Li J, Ma S, Cheng L. Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/ polyamide composite scaffolds for bone tissue engineering. Biomaterials. 2007;28:3338–3348.
- Venugopal J, Low S, Choon AT, Sampath Kumar TS, Ramakrishna S. Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers. J Mater Sci Mater Med. 2008;19:2039–2046.
- Shi S, Robey P, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001;29:532–539.
- Nagatomo K, Komaki M, Sekiya I, et al. Stem cell properties of human periodontal ligament cells. J Periodontal Res. 2006;41:303–310.
- Seo BM, Sonoyama W, Yamaza T, et al. SHED repair critical-size calvarial defects in mice. Oral Dis. 2008;14:428–434.
Serial posts:
- Porous collagen-hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration
- Abstract: Porous collagen-hydroxyapatite scaffolds ...
- Introduction: Porous collagen-hydroxyapatite scaffolds ...
- Materials & methods: Porous collagen-hydroxyapatite scaffolds ...
- Results: Porous collagen-hydroxyapatite scaffolds ...
- Discussion: Porous collagen-hydroxyapatite scaffolds ...
- Conclusions: Porous collagen-hydroxyapatite scaffolds ...