hPDSCs may be a suitable resource of cells for maxillofacial and alveolar bone regeneration.
Conclusions: Porous collagen-hydroxyapatite scaffolds ...
author: Li Ning, DDS, PhDHans Malmstrm, DDSYan-Fang Ren, DDS, MPH, PhD | publisher: drg. Andreas Tjandra, Sp. Perio, FISID
Conclusions
The porous Col-HA composites developed in the present study are biocompatible and can be used as scaffolds for bone tissue regeneration. The Col-HA ratio is an important factor in promoting the attachment and proliferation of mouse MSCs. The Col-HA composite complexes have strong potentials in bone tissue regeneration applications. hPDSCs may be a suitable resource of cells for maxillofacial and alveolar bone regeneration.
Abbreviations
Col
collagen
DMEM
Dulbecco's Modified Eagle Medium
HA
hydroxyapatite
hPDSCs
human periodontal stem cells
MSCs
mesenchymal stem cells
SEM
scanning electron microscope
Acknowledgments
This study was supported by American Academy of Implant Dentistry (AAID) Research.
References
- Janicki P, Boeuf S, Steck E, Egermann M, Kasten P, Richter W. Prediction of in vivo bone forming potency of bone marrow-derived human mesenchymal stem cells. Eur Cell Mater. 2011;21:488–507.
- Prescott RS, Alsanea R, Fayad MI, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen Scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–426.
- Hutmacher DW, Garcia AJ. Scaffold-based bone engineering by using genetically modified cells. Gene. 2005;347:1–10.
- Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002;84-A:454–464.
- Norton MR, Odell EW, Thompson ID, Cook RJ. Efficacy of bovine bone mineral for alveolar augmentation: a human histologic study. Clin Oral Implants Res. 2003;14:775–783.
- Hoexter DL. Bone regeneration graft materials. J Oral Implant. 2002;28:290–294.
- Miranda SC, Silva GA, Mendes RM, et al. Mesenchymal stem cells associated with porous chitosan-gelatin scaffold: a potential strategy for alveolar bone regeneration. J Biomed Mater Res Part A. 2012;100:2775–2786.
- El-Ghannam A. Bone reconstruction: from bioceramics to tissue engineering. Expert Rev Med Devices. 2005;2:87–101.
- Henderson JA, He X, Jabbari E. Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 2008;8:499–507.
- Bose S, Roy M, Bandyopadhyay A. Recent advances in bone tissue engineering scaffolds. Trends Biotechnol. 2012;30:546–554.
- Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT. Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg. 2008;90(suppl 1):36–42.
- Yunoki S, Ikoma T, Tsuchiya A, et al. Fabrication and mechanical and tissue ingrowth properties of unidirectionally porous hydroxyapatite/collagen composite. J Biomed Mater Res Part B Appl Biomater. 2007;80B:166–173.
- Wahl DA, Czernuszka JT. Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater. 2006;11:43–56.
- Zhang SM, Cui FZ, Liao SS, Zhu Y, Han L. Synthesis and biocompatibility of porous nano-hydroxyapatite/collagen/alginate composite. J Mater Sci Mater Med. 2003;14:641–645.
- Zhu X, Eibl O, Scheideler L, Geis-Gerstorfer J. Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors. J Biomed Mater Res Part A. 2006;79:114–127.
- Chan CK, Kumar TS, Liao S, Murugan R, Ngiam M, Ramakrishnan S. Biomimetic nanocomposites for bone graft applications. Nanomedicine (Lond). 2006;1:177–188.
- Detsch R, Uhl F, Deisinger U, Ziegler G. 3D-Cultivation of bone marrow stromal cells on hydroxyapatite scaffolds fabricated by dispense-plotting and negative mould technique. J Mater Sci Mater Med. 2008;19:1491–1496.
- Liao S, Wang W, Uo M, et al. A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials. 2005;26:7564–7571.
- Kikuchi M, Matsumoto HN, Yamada T, Koyama Y, Takakuda K, Tanaka J. Glutaraldehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites. Biomaterials. 2004;25:63–69.
- Geiger M. Porous Collagen/Ceramic Composite Carriers for Bone Regeneration Using Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) [dissertation]. Erlangen-Nuremberg, Germany: University of Erlangen-Nuremberg; 2001.
- Wang H, Li Y, Zuo Y, Li J, Ma S, Cheng L. Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/ polyamide composite scaffolds for bone tissue engineering. Biomaterials. 2007;28:3338–3348.
- Venugopal J, Low S, Choon AT, Sampath Kumar TS, Ramakrishna S. Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers. J Mater Sci Mater Med. 2008;19:2039–2046.
- Shi S, Robey P, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001;29:532–539.
- Nagatomo K, Komaki M, Sekiya I, et al. Stem cell properties of human periodontal ligament cells. J Periodontal Res. 2006;41:303–310.
- Seo BM, Sonoyama W, Yamaza T, et al. SHED repair critical-size calvarial defects in mice. Oral Dis. 2008;14:428–434.
Serial posts:
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Porous collagen-hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration
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Abstract: Porous collagen-hydroxyapatite scaffolds ...
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Introduction: Porous collagen-hydroxyapatite scaffolds ...
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Materials & methods: Porous collagen-hydroxyapatite scaffolds ...
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Results: Porous collagen-hydroxyapatite scaffolds ...
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Discussion: Porous collagen-hydroxyapatite scaffolds ...
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Conclusions: Porous collagen-hydroxyapatite scaffolds ...
Conclusions
The porous Col-HA composites developed in the present study are biocompatible and can be used as scaffolds for bone tissue regeneration. The Col-HA ratio is an important factor in promoting the attachment and proliferation of mouse MSCs. The Col-HA composite complexes have strong potentials in bone tissue regeneration applications. hPDSCs may be a suitable resource of cells for maxillofacial and alveolar bone regeneration.
Abbreviations
| Col |
collagen |
| DMEM |
Dulbecco's Modified Eagle Medium |
| HA |
hydroxyapatite |
| hPDSCs |
human periodontal stem cells |
| MSCs |
mesenchymal stem cells |
| SEM |
scanning electron microscope |
Acknowledgments
This study was supported by American Academy of Implant Dentistry (AAID) Research.
References
- Janicki P, Boeuf S, Steck E, Egermann M, Kasten P, Richter W. Prediction of in vivo bone forming potency of bone marrow-derived human mesenchymal stem cells. Eur Cell Mater. 2011;21:488–507.
- Prescott RS, Alsanea R, Fayad MI, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen Scaffold, and dentin matrix protein 1 after subcutaneous transplantation in mice. J Endod. 2008;34:421–426.
- Hutmacher DW, Garcia AJ. Scaffold-based bone engineering by using genetically modified cells. Gene. 2005;347:1–10.
- Finkemeier CG. Bone-grafting and bone-graft substitutes. J Bone Joint Surg Am. 2002;84-A:454–464.
- Norton MR, Odell EW, Thompson ID, Cook RJ. Efficacy of bovine bone mineral for alveolar augmentation: a human histologic study. Clin Oral Implants Res. 2003;14:775–783.
- Hoexter DL. Bone regeneration graft materials. J Oral Implant. 2002;28:290–294.
- Miranda SC, Silva GA, Mendes RM, et al. Mesenchymal stem cells associated with porous chitosan-gelatin scaffold: a potential strategy for alveolar bone regeneration. J Biomed Mater Res Part A. 2012;100:2775–2786.
- El-Ghannam A. Bone reconstruction: from bioceramics to tissue engineering. Expert Rev Med Devices. 2005;2:87–101.
- Henderson JA, He X, Jabbari E. Concurrent differentiation of marrow stromal cells to osteogenic and vasculogenic lineages. Macromol Biosci. 2008;8:499–507.
- Bose S, Roy M, Bandyopadhyay A. Recent advances in bone tissue engineering scaffolds. Trends Biotechnol. 2012;30:546–554.
- Khan Y, Yaszemski MJ, Mikos AG, Laurencin CT. Tissue engineering of bone: material and matrix considerations. J Bone Joint Surg. 2008;90(suppl 1):36–42.
- Yunoki S, Ikoma T, Tsuchiya A, et al. Fabrication and mechanical and tissue ingrowth properties of unidirectionally porous hydroxyapatite/collagen composite. J Biomed Mater Res Part B Appl Biomater. 2007;80B:166–173.
- Wahl DA, Czernuszka JT. Collagen-hydroxyapatite composites for hard tissue repair. Eur Cell Mater. 2006;11:43–56.
- Zhang SM, Cui FZ, Liao SS, Zhu Y, Han L. Synthesis and biocompatibility of porous nano-hydroxyapatite/collagen/alginate composite. J Mater Sci Mater Med. 2003;14:641–645.
- Zhu X, Eibl O, Scheideler L, Geis-Gerstorfer J. Characterization of nano hydroxyapatite/collagen surfaces and cellular behaviors. J Biomed Mater Res Part A. 2006;79:114–127.
- Chan CK, Kumar TS, Liao S, Murugan R, Ngiam M, Ramakrishnan S. Biomimetic nanocomposites for bone graft applications. Nanomedicine (Lond). 2006;1:177–188.
- Detsch R, Uhl F, Deisinger U, Ziegler G. 3D-Cultivation of bone marrow stromal cells on hydroxyapatite scaffolds fabricated by dispense-plotting and negative mould technique. J Mater Sci Mater Med. 2008;19:1491–1496.
- Liao S, Wang W, Uo M, et al. A three-layered nano-carbonated hydroxyapatite/collagen/PLGA composite membrane for guided tissue regeneration. Biomaterials. 2005;26:7564–7571.
- Kikuchi M, Matsumoto HN, Yamada T, Koyama Y, Takakuda K, Tanaka J. Glutaraldehyde cross-linked hydroxyapatite/collagen self-organized nanocomposites. Biomaterials. 2004;25:63–69.
- Geiger M. Porous Collagen/Ceramic Composite Carriers for Bone Regeneration Using Recombinant Human Bone Morphogenetic Protein-2 (rhBMP-2) [dissertation]. Erlangen-Nuremberg, Germany: University of Erlangen-Nuremberg; 2001.
- Wang H, Li Y, Zuo Y, Li J, Ma S, Cheng L. Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/ polyamide composite scaffolds for bone tissue engineering. Biomaterials. 2007;28:3338–3348.
- Venugopal J, Low S, Choon AT, Sampath Kumar TS, Ramakrishna S. Mineralization of osteoblasts with electrospun collagen/hydroxyapatite nanofibers. J Mater Sci Mater Med. 2008;19:2039–2046.
- Shi S, Robey P, Gronthos S. Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone. 2001;29:532–539.
- Nagatomo K, Komaki M, Sekiya I, et al. Stem cell properties of human periodontal ligament cells. J Periodontal Res. 2006;41:303–310.
- Seo BM, Sonoyama W, Yamaza T, et al. SHED repair critical-size calvarial defects in mice. Oral Dis. 2008;14:428–434.
- Porous collagen-hydroxyapatite scaffolds with mesenchymal stem cells for bone regeneration
- Abstract: Porous collagen-hydroxyapatite scaffolds ...
- Introduction: Porous collagen-hydroxyapatite scaffolds ...
- Materials & methods: Porous collagen-hydroxyapatite scaffolds ...
- Results: Porous collagen-hydroxyapatite scaffolds ...
- Discussion: Porous collagen-hydroxyapatite scaffolds ...
- Conclusions: Porous collagen-hydroxyapatite scaffolds ...