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Discussion: Advanced Platelet-Rich Fibrin

Discussion Complex tissue engineering concepts have to be evaluated in terms of their clinical applicability. Thus, the overall goal should be to establish a method that could ideally be completed within a short time span before or during intended regenerative surgical procedures. Over the last few years, Choukroun's PRF has proven to be a method that comes close to the ideal concept of guide...

Results: Advanced Platelet-Rich Fibrin

Results Histochemical studies (H&E, Mason-Goldner, and Giemsa) S-PRF In the longitudinal section of the S-PRF clot, produced according to the standard centrifugation protocol (2700 rpm, 12 minutes), a dense fibrin clot was seen with minimal interfibrous space. With the standard histochemical staining methods, cells were observed throughout the clot, albeit decreasing toward the more distal ...

Material and Methods: Advanced Platelet-Rich Fibri...

Production of Choukroun's PRF The PRF scaffolds were prepared according to a previously published protocol.11,26  Four healthy (ie, with no history of anticoagulant usage) volunteers in an age range between 18 to 60 years participated in this study. For each individual, 4 tubes of peripheral blood were collected and immediately placed in a preprogrammed centrifuge (PC-O2, PROCESS for PRF, N...

Introduction: Advanced Platelet-Rich Fibrin

A major objective of biomaterial research and tissue engineering is to promote a material-induced tissue reaction that leads to regeneration and an effective wound-healing process in the defective area. Thus, a biomaterial should serve as a temporary barrier to cover defects and promote tissue regeneration while being tissue compatible and, most importantly, clinically applicable. In the field o...

Advanced Platelet-Rich Fibrin

Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells  Shahram Ghanaati, MD, DMD; Patrick Booms, PhD; Anna Orlowska, BSc, DVM; Alica Kubesch; Jonas Lorenz, DDS; Jim Rutkowski, DMD, PhD; Constantin Landes, MD, DMD, PhD; Robert Sader, MD, DDS, PhD; CJ Kirkpatrick, MD, PhD, DSc; Joseph Choukroun, MD   J Oral Impla...

Pendahuluan: Perancah kolagen-hidroksiapatit berpo...

Pendahuluan Menggabungkan perancah dan sel hidup untuk membentuk konstruksi rekayasa jaringan adalah konsep yang penting untuk memajukan perbaikan dan regenerasi jaringan tulang. Sel induk mesenkim sering digunakan dalam konstruksi seperti itu karena kemampuannya untuk berkembangbiak dan berdiferensiasi menuju pembentuk sel tulang. Desain dan pembuatan perancah, isolasi dan karakterisasi sel indu...

Conclusions: Porous collagen-hydroxyapatite scaffo...

Conclusions The porous Col-HA composites developed in the present study are biocompatible and can be used as scaffolds for bone tissue regeneration. The Col-HA ratio is an important factor in promoting the attachment and proliferation of mouse MSCs. The Col-HA composite complexes have strong potentials in bone tissue regeneration applications. hPDSCs may be a suitable resource of cells for maxi...

Discussion: Porous collagen-hydroxyapatite scaffol...

Discussion The findings of the presented study indicate that the porous sponge-like Col-HA composites have good biocompatibility and biomimetic properties and may be used as scaffolds for bone tissue regeneration. The Col-HA composites with ratios 80:20 and 50:50 supported the attachments and proliferations of mouse MSCs and hPDSCs. These findings indicate that Col-HA composite complexes have str...

Results: Porous collagen-hydroxyapatite scaffolds ...

Results The sponge-like plugs of prototype Col-HA composites were successfully fabricated with different collagen and HA ratios. The macroscopic and SEM views of the prototype type I collagen without HA and 3 different ratios of collagen-HA (20%Col-80%HA; 50%Col-50%HA; 80%Col-20%HA) composites are shown in Figure 1. The SEM views show the inside microstructures of the prototype pure type I colla...

Materials & methods: Porous collagen-hydroxyapatit...

Materials and Methods Synthesis of the Col-HA composites by direct precipitation in situ Solutions of calcium salt and phosphoric acid (Ca/P = 1.66 mol) were used to synthesize HA particles and incorporate them on bovine type I collagen fibrils by a direct precipitation technique in situ. This technique was optimized to produce 3 different ratios of Col-HA composites (20%Col-80%HA; 50%Col-50%H...

Introduction: Porous collagen-hydroxyapatite scaff...

Introduction Combining a scaffold and living cells to form a tissue-engineering construct is an important concept for promoting the repair and regeneration of bone tissues. Mesenchymal stem cells are often used in such constructs due to their abilities to proliferate and differentiate toward bone-forming cells. The design and fabrication of scaffolds, stem cell isolation and characterization, and...

Abstract: Porous collagen-hydroxyapatite scaffolds...

Abstract Current bone grafting materials have significant limitations for repairing maxillofacial and dentoalveolar bone deficiencies. An ideal bone tissue-engineering construct is still lacking. The purpose of the present study was first to synthesize and develop a collagen-hydroxyapatite (Col-HA) composite through controlled in situ mineralization on type I collagen fibrils with nanometer-sized...

Porous collagen-hydroxyapatite scaffolds with mese...

  RESEARCH Porous Collagen-Hydroxyapatite Scaffolds With Mesenchymal Stem Cells for Bone Regeneration Li Ning, DDS, PhD , Hans Malmström, DDS , Yan-Fang Ren, DDS, MPH, PhD Correspondence: * Corresponding author, e-mail: yanfang_ren@urmc.rochester.edu Article Citation: Li Ning, Hans Malmström, Yan-Fang Ren, Porous Collagen-Hydroxyapatite Scaffolds With Mesench...