Discussion Complex tissue engineering concepts have to be evaluated in terms of their clinical applicability. Thus, the overall goal should be to establish a method that could ideally be completed within a short time span before or during intended regenerative surgical procedures. Over the last few years, Choukroun's PRF has proven to be a method that comes close to the ideal concept of guide...

Results Histochemical studies (H&E, Mason-Goldner, and Giemsa) S-PRF In the longitudinal section of the S-PRF clot, produced according to the standard centrifugation protocol (2700 rpm, 12 minutes), a dense fibrin clot was seen with minimal interfibrous space. With the standard histochemical staining methods, cells were observed throughout the clot, albeit decreasing toward the more distal ...

Production of Choukroun's PRF The PRF scaffolds were prepared according to a previously published protocol.11,26  Four healthy (ie, with no history of anticoagulant usage) volunteers in an age range between 18 to 60 years participated in this study. For each individual, 4 tubes of peripheral blood were collected and immediately placed in a preprogrammed centrifuge (PC-O2, PROCESS for PRF, N...

A major objective of biomaterial research and tissue engineering is to promote a material-induced tissue reaction that leads to regeneration and an effective wound-healing process in the defective area. Thus, a biomaterial should serve as a temporary barrier to cover defects and promote tissue regeneration while being tissue compatible and, most importantly, clinically applicable. In the field o...

Advanced Platelet-Rich Fibrin: A New Concept for Cell-Based Tissue Engineering by Means of Inflammatory Cells  Shahram Ghanaati, MD, DMD; Patrick Booms, PhD; Anna Orlowska, BSc, DVM; Alica Kubesch; Jonas Lorenz, DDS; Jim Rutkowski, DMD, PhD; Constantin Landes, MD, DMD, PhD; Robert Sader, MD, DDS, PhD; CJ Kirkpatrick, MD, PhD, DSc; Joseph Choukroun, MD   J Oral Impla...