References : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [3]
Hughes-Fulford M. Signal transduction and mechanical stress. Sci STKE. 2004;2004(249):RE12.
Ruel J, Lemay J, Dumas G, Doillon C, Charara J. Development of a parallel plate flow chamber for studying cell behavior under pulsatile flow. ASAIO J. 1995;41(4):876–83.
Kazakidi A, Sherwin SJ, Weinberg PD. Effect of Reynolds number and flow division on patterns of haemodynamic wall shear stress near branch points in the descending thoracic aorta. J R Soc Interface. 2009;6(35):539–48.
Brown DC, Larson RS. Improvements to parallel plate flow chambers to reduce reagent and cellular requirements. BMC Immunol. 2001;2:9.
Usami S, Chen HH, Zhao Y, Chien S, Skalak R. Design and construction of a linear shear stress flow chamber. Ann Biomed Eng. 1993;21(1):77–83.
Sikavitsas VI, Bancroft GN, Holtorf HL, Jansen JA, Mikos AG. Mineralized matrix deposition by marrow stromal osteoblasts in 3D perfusion culture increases with increasing fluid shear forces. Proc Natl Acad Sci U S A. 2003;100(25):14683–8.
Mcgarry JG, Klein-Nulend J, Prendergast PJ. The effect of cytoskeletal disruption on pulsatile fluid flow-induced nitric oxide and prostaglandin E2 release in osteocytes and osteoblasts. Biochem Biophys Res Commun. 2005;330(1):341–8.
Carvalho RS, Scott JE, Yen EH. The effects of mechanical stimulation on the distribution of beta 1 integrin and expression of beta 1-integrin mRNA in TE-85 human osteosarcoma cells. Arch Oral Biol. 1995;40(3):257–64.
Malone AM, Batra NN, Shivaram G, Kwon RY, You L, Kim CH, et al. The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts. Am J Physiol Cell Physiol. 2007;292(5):C1830–6.
Lichtenstein N, Geiger B, Kam Z. Quantitative analysis of cytoskeletal organization by digital fluorescent microscopy. Cytometry A. 2003;54(1):8–18.
Jacobs CR, Yellowley CE, Davis BR, Zhou Z, Cimbala JM, Donahue HJ. Differential effect of steady versus oscillating flow on bone cells. J Biomech. 1998;31(11):969–76.
Srinivasan S, Agans SC, King KA, Moy NY, Poliachik SL, Gross TS. Enabling bone formation in the aged skeleton via rest-inserted mechanical loading. Bone. 2003;33(6):946–55.
Serial posts:
- Abstract : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Background : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- Background : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- Methods : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- Methods : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- Results : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- Results : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- Discussion : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- Discussion : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- Discussion : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [3]
- Conclusions : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Abbreviations : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- References : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- References : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- References : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [3]
- References : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [4]
- Acknowledgements : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Author information : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [1]
- Author information : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up [2]
- Rights and permissions : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- About this article : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Table 1 Listing of the single components of the flow chamber together with manufacturers’ data : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Table 2 Listing of the culture media and additives together with manufacturers’ data : Cellular fluid shear stress on implant surfaces—establishment of a novel experimental set up
- Fig. 1. Three-dimensional illustration (a–e) and photography (f) of the experimental setup with the components marked numerical. a1 Lower petri dish (s’ bottom serving as the lower plate); 2 Rotating glass panel [60 mm diameter (cell bearing)]; 3 Titanium axis. b4 Liquid medium (red). c5 Reversed upper petri dish. d6 Gearwheel with set screw. e7 Closing; 8 Electronic motor device and adjusting ring with additional set screw : Cellular fluid shear stress on implant
- Fig. 2. Side view of a computerized simulation, showing the flow chambers’ lower compartment and the flow profile in between the two plates; shearing gap and bottom plate are shown on the left side; rotation speed = 200 rpm; colour code bar (left edge) showing shear force values [Pa] [1 Pa = 10 dyn/cm2]; flow direction presented by arrows : Cellular fluid shear stress on implant
- Fig. 3. Diagram for visualisation of the calculation of shear stress rates taking into account the centrifugal force and the glass plates’ dimensions. For example, at a distance of 25 mm from the centre of the upper plate, the shear forces’ value is 8.33 dyn/cm2, together with an additional centrifugal force that has a value of 0.55 dyn/cm2 : Cellular fluid shear stress on implant
- Fig. 4. Randomly orientated osteoblasts without influence of rotation (phallacidin fluorescence staining). On the left side with 200× and on the right side with 400× magnification. The white X on the coloured circle marks the location upon the plate where the osteoblasts were located. The red X marks the centre of the plate : Cellular fluid shear stress on implant
- Fig. 5. Osteoblasts with an orientation tendency after 24 h of rotation (phallacidin fluorescence staining). On the left side with 200× and on the right side with 400× magnification. The yellow arrows show the orientation of the cells. The red arched arrow within the coloured circle shows the direction of rotation. The dashed white line oriented to the right stands for the resulting centrifugal force. The dashed white line pointing upwards shows the direction of the resulting flow resistance. The solid white arrow stands for the vectorial sum of the abovementioned forces : Cellular fluid shear stress on implant