Kanazawa, M., Atsuta, I., Ayukawa, Y. et al. The influence of systemically or locally administered mesenchymal stem cells on tissue repair in a rat oral implantation model.
Int J Implant Dent 4, 2 (2018). https://doi.org/10.1186/s40729-017-0112-4
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Received: 19 September 2017
Accepted: 04 December 2017
Published: 13 January 2018
DOI: https://doi.org/10.118...
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Miya Kanazawa, Ikiru Atsuta, Yasunori Ayukawa, Takayoshi Yamaza, Ryosuke Kondo, Yuri Matsuura, and Kiyoshi Koyano declare that they have no competing interests.
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Correspondence to
Ikiru Atsuta.
Section of Implant and Rehabilitative Dentistry, Division of Oral Rehabilitation, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan
Miya Kanazawa, Ikiru Atsuta, Yasunori Ayukawa, Ryosuke Kondo, Yuri Matsuura & Kiyoshi Koyano
Department of Molecular Cell and Oral Anatomy, Faculty of Dental Science, Kyushu University, Fukuoka, Japan
Takayoshi...
This work was supported by JSPS KAKENHI Grant Numbers JP 15H05029 and 15H02573 to Y. Ayukawa.
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Our study supports systemic administration of MSCs to enable accelerated soft tissue sealing of the Ti surface in our rat oral implantation model. Although local MSC administration had little positive effect in our model, the MSCs accumulated around the peri-implant oral mucosa and were identified in various organs, indicating a wide range of possible applications. This study highlights that clini...
Figure 7C and D showed the suitable amount of MSCs had much better positive effect for the migration and adhesion of OECs to titanium surface. MSC attachment within the upper Transwell chamber was determined 24 h after seeding by fluorescence microscopy, as shown in Fig. 7D (a). The majority of MSCs appeared flattened with numerous cytoplasmic extensions and lamellipodia. The majority of MSCs pass...
Due to the existence of muscles, connective tissue, dermal layer, and basement membrane, cells within the mass of the injected area encounter these barriers, inhibiting the distance of migration between the application region and inflammatory site, which has an estimated diameter of 20–30 μm (Fig. 6). High-density cell injection at the topical region is also an obstacle for homing, thus using a...
Subcutaneously administrated cells or drugs are reported to take a few days to be delivered into the body through vessel bloods [33, 34]. This may be owed to difficulty of the cells in securing vascular accesses to the target site because of a lack of blood vessels at the buccinators, while systemic MSC homing occurs more readily through the bloodstream [35].
The effects of MSC treatment on level...
As shown in Fig. 3b, expression of adhesion proteins on the interface between PIE-implant was significantly lower in the control and local groups compared with the systemic group.
Ln-332 is the major adhesive ligand for integrin α6β4, which interacts with the cytoskeletal elements, and is a component of the hemidesmosomes, epithelial adhesion plaques that tack the plasma membrane of the epithe...
Because MSC treatment is being introduced more widely as a clinically available therapy, the method of administration must be considered to better mitigate risk. Although for a number of other factors also need consideration, including cell source, cell donor condition, cell population, and timing of MSC administration, this study only focused on comparison between systemic and local injection of ...
OEC adhesion assays were conducted according to previously published methods [16, 20]. Non-adherent or weakly attached cells were removed by shaking (3 × 5 min at 75 rpm) using a rotary shaker (NX-20, Nissin, Tokyo, Japan). Adherent cells were then counted and calculated as a percentage of the initial count, which was used to define adhesive strength of the cells.
Scratch assays were perfo...
For apoptosis detection, the 10-μm bucco-palatal sections from around the experimental implant were incubated overnight with FITC-conjugated anti-rat GFP (1:100, Sigma-Aldrich) and 7-amino actinomycin D (7-AAD, Apoptosis Detection Kit; BD Biosciences, Franklin Lakes, NJ) at 4 °C. Apoptotic cells were then counted and calculated as a percentage of the total cells.
MSCs were cultured in osteogen...
Twenty-four hours after implantation, rats were lightly anesthetized with chloral hydrate and lidocaine hydrochloride, and ex vivo expanded P3 green fluorescent protein (GFP)-MSCs (1 × 106 cells) were administrated via one of the following modes: (1) systemic injection via the tail vein (systemic group), (2) local injection into the gingivobuccal fold around the dental implant (local group), ...
Bone marrow cells were flushed out of the femurs and tibias of 4-week-old green fluorescent protein-transgenic Wistar rats. Cells were treated with a 0.85% NH4Cl solution for 10 min to lyse the red blood cells and were passed through a 70-μm cell strainer to obtain a single cell suspension. Cells were seeded into 100-mm plastic culture dishes (1 × 106 cells/dish), washed with phosphate buff...
The purpose of this study was to verify the effects and mechanisms of bone marrow-derived MSCs following their local administration using an oral implantation rat model, to deepen our understanding of this approach for effective utilization of MSCs.
Mesenchymal stem cell (MSC)-based approaches can be broadly divided into two categories: cell therapy and regenerative medicine. Cell therapy is focused on the anti-inflammatory, immune-regulatory, and homeostasis-regulatory actions of MSCs to treat disorders like malignant lymphoma, angina pectoris, and atopic dermatitis. Conversely, regenerative medicine is focused on MSCs playing a tissue engin...
Multipotent mesenchymal stem cells (MSCs) are used clinically in regenerative medicine. Our previous report showed systemically injected MSCs improved peri-implant sealing and accelerated tissue healing. However, the risks of systemic MSC administration, including lung embolism, must be considered; therefore, their local application must be assessed for clinical safety and efficacy. We investigate...