Additional information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
Additional information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
author: Martin Gosau, Sandra Viale-Bouroncle, Hannah Eickhoff, Esthera Prateeptongkum, Anja Reck, W Gtz, Christoph Klingelhffer, Steffen | publisher: drg. Andreas Tjandra, Sp. Perio, FISID
Martin Gosau, Sandra Viale-Bouroncle, Hannah Eickhoff, Esthera Prateeptongkum, Anja Reck, W Götz, Christoph Klingelhöffer, Steffen Müller and Christian Morsczeck declare that they have no competing interests.
SVB, HE, and EP carried out all cell biology experiments, performed real-time PCRs, Western blots, and the statistical analysis and made figures for the manuscript. AR carried out and analyzed the flow cytometry experiments and made figures. SM participated in study design and supervised the research team. MG, CK, and CM designed experiments, supervised the research team, and prepared the manuscript. WG evaluated the histology of dental stem cells on biomaterials. All authors read and approved final version of the manuscript.
Serial posts:
- Abstract : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Background : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Background : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [3]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [4]
- Results : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Results : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Conclusions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [3]
- Acknowledgement : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Author information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Author information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Additional information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Additional file : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Rights and permissions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- About this article : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Figure 1. Cell attachment on tested materials. (A) Relative cell adherence of DFCs and dNC-PCs; (B) dental cells did little adhere on PA; representative pictures of DFCs. : Evaluation of implant
- Figure 2. Cell proliferation of dNC-PCs and DFCs on tested materials. (A) and (B) Relative cell numbers; (C) spheroid cell clusters on silicone (representative pictures for DFCs); Silicone (24 and 48 h). : Evaluation of implant
- Figure 3. Evaluation of programmed cell death (apoptosis) in dental stem cells. (A) Flow cytometry analyses (for details materials and methods) show percentage of vital cells (black number), apoptotic cells (blue number), and dead cells (red number). (B) Western blot analyses show the expression of the pro-apoptotic marker BAX and the anti-apoptotic marker BCL2. : Evaluation of implant
- Figure 4. Osteogenic differentiation of dental stem cells. Normalized ALP activity of dNC-PCs and DFCs on AP and SB (A) and on silicone (B). Cells were differentiated on standard cell culture dishes for control. : Evaluation of implant
- Figure 5. Evaluation of osteogenic differentiation. (A) Clustergram of PCR-array results; (B-C) histology of differentiated dental cells on AP (B) and SB (C). Representative results are shown for dNC-PCs. : Evaluation of implant
- Figure 6. Cultivation and osteogenic differentiation of DFCs on PA after modification with collagen I. (Left) Relative cell number and (Right) normalized ALP activity. : Evaluation of implant