Conclusions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
Conclusions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
author: Martin Gosau, Sandra Viale-Bouroncle, Hannah Eickhoff, Esthera Prateeptongkum, Anja Reck, W Gtz, Christoph Klingelhffer, Steffen | publisher: drg. Andreas Tjandra, Sp. Perio, FISID
In conclusion, our work supports our hypothesis that soft implant materials are not suitable for dental tissue engineering. Moreover, our study also supports the results of our previous studies with DFCs and TCP that induction of apoptosis did not impair the proliferation and the differentiation in dental stem cells.
Serial posts:
- Abstract : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Background : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Background : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [3]
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [4]
- Results : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Results : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Conclusions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- References : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [3]
- Acknowledgement : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Author information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [1]
- Author information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions [2]
- Additional information : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Additional file : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Rights and permissions : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- About this article : Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Figure 1. Cell attachment on tested materials. (A) Relative cell adherence of DFCs and dNC-PCs; (B) dental cells did little adhere on PA; representative pictures of DFCs. : Evaluation of implant
- Figure 2. Cell proliferation of dNC-PCs and DFCs on tested materials. (A) and (B) Relative cell numbers; (C) spheroid cell clusters on silicone (representative pictures for DFCs); Silicone (24 and 48 h). : Evaluation of implant
- Figure 3. Evaluation of programmed cell death (apoptosis) in dental stem cells. (A) Flow cytometry analyses (for details materials and methods) show percentage of vital cells (black number), apoptotic cells (blue number), and dead cells (red number). (B) Western blot analyses show the expression of the pro-apoptotic marker BAX and the anti-apoptotic marker BCL2. : Evaluation of implant
- Figure 4. Osteogenic differentiation of dental stem cells. Normalized ALP activity of dNC-PCs and DFCs on AP and SB (A) and on silicone (B). Cells were differentiated on standard cell culture dishes for control. : Evaluation of implant
- Figure 5. Evaluation of osteogenic differentiation. (A) Clustergram of PCR-array results; (B-C) histology of differentiated dental cells on AP (B) and SB (C). Representative results are shown for dNC-PCs. : Evaluation of implant
- Figure 6. Cultivation and osteogenic differentiation of DFCs on PA after modification with collagen I. (Left) Relative cell number and (Right) normalized ALP activity. : Evaluation of implant