Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
Abstract
Background
Dental stem cells in combination with implant materials may become an alternative to autologous bone transplants. For tissue engineering different types of soft and rigid implant materials are available, but little is known about the viability and the osteogenic differentiation of dental stem cells on these different types of materials. According to previous studies we proposed that rigid bone substitute materials are superior to soft materials for dental tissue engineering.
Methods
We evaluated the proliferation, the induction of apoptosis and the osteogenic differentiation of dental stem/progenitor cells on a synthetic bone-like material and on an allograft product. The soft materials silicone and polyacrylamide (PA) were used for comparison. Precursor cells from the dental follicle (DFCs) and progenitor cells from the dental apical papilla of retained third molar tooth (dNC-PCs) were applied as dental stem cells in our study.
Results
Both dental cell types attached and grew on rigid bone substitute materials, but they did not grow on soft materials. Moreover, rigid bone substitute materials only sustained the osteogenic differentiation of dental stem cells, although the allograft product induced apoptosis in both dental cell types. Remarkably, PA, silicone and the synthetic bone substitute material did not induce the apoptosis in dental cells.
Conclusions
Our work supports the hypothesis that bone substitute materials are suitable for dental stem cell tissue engineering. Furthermore, we also suggest that the induction of apoptosis by bone substitute materials may not impair the proliferation and the differentiation of dental stem cells.
Serial posts:
- Evaluation of implant-materials as cell carriers for dental stem cells under in vitro conditions
- Background : Evaluation of implant-materials as cell carriers for dental stem cells
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells (1)
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells (2)
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells (3)
- Methods : Evaluation of implant-materials as cell carriers for dental stem cells (4)
- Results : Evaluation of implant-materials as cell carriers for dental stem cells
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells (1)
- Discussion : Evaluation of implant-materials as cell carriers for dental stem cells (2)
- Figure 1. Cell attachment on tested materials.
- Figure 2. Cell proliferation of dNC-PCs and DFCs on tested materials
- Figure 3. Evaluation of programmed cell death (apoptosis) in dental stem cells
- Figure 4. Osteogenic differentiation of dental stem cells
- Figure 5. Evaluation of osteogenic differentiation
- Figure 6. Cultivation and osteogenic differentiation of DFCs on PA