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Figure 4. a C2C12 cells (control) and pBMP-2-transfected C2C12 cells were seeded on a 24-well plate (50,000 cell/well) and cultured in DMEM medium for 48 h. ALPL assay showing upregulated ALPL activity in the BMP-2-transfected C2C12 cells. b Cell extracts of C2C12 cells and pBMP-2-transfected cells were run on a 10% SDS-PAGE under non-denaturing conditions. The gel was then stained with NBT/BCIP solution (upper panel). Western bloting of actin showing the equal protein loading on the gel (lower panel). c Increased proliferation of C2C12 cells transfected with BMP-2 as well as ALPL activity when seeded on 3M™ESPE™ MDI disks. However, when the number of ALPL-positive cells is normalized to the total cell number, no differences are observed

Figure 4. a C2C12 cells (control) and pBMP-2-transfected C2C12 cells

author: Jagjit Singh Dhaliwal,Juliana Marulanda,Jingjing Li,Sharifa Alebrahim,Jocelyne Sheila Feine, Monzur Murshed | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

 

Figure 4. a C2C12 cells (control) and pBMP-2-transfected C2C12 cells were seeded on a 24-well plate (50,000 cell/well) and cultured in DMEM medium for 48 h. ALPL assay showing upregulated ALPL activity in the BMP-2-transfected C2C12 cells. b Cell extracts of C2C12 cells and pBMP-2-transfected cells were run on a 10% SDS-PAGE under non-denaturing conditions. The gel was then stained with NBT/BCIP solution (upper panel). Western bloting of actin showing the equal protein loading on the gel (lower panel). c Increased proliferation of C2C12 cells transfected with BMP-2 as well as ALPL activity when seeded on 3M™ESPE™ MDI disks. However, when the number of ALPL-positive cells is normalized to the total cell number, no differences are observed
Figure 4. a C2C12 cells (control) and pBMP-2-transfected C2C12 cells were seeded on a 24-well plate (50,000 cell/well) and cultured in DMEM medium for 48 h. ALPL assay showing upregulated ALPL activity in the BMP-2-transfected C2C12 cells. b Cell extracts of C2C12 cells and pBMP-2-transfected cells were run on a 10% SDS-PAGE under non-denaturing conditions. The gel was then stained with NBT/BCIP solution (upper panel). Western bloting of actin showing the equal protein loading on the gel (lower panel). c Increased proliferation of C2C12 cells transfected with BMP-2 as well as ALPL activity when seeded on 3M™ESPE™ MDI disks. However, when the number of ALPL-positive cells is normalized to the total cell number, no differences are observed

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Share: Posted 2021-02-11 05:59:12
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