Figure 5. a Florescence microscopy showing H33258-stained MC3T3-E1 cells on Ankylos® and 3M™ESPE™ MDI disks. Although equal numbers of cells were plated, after 12 days of culture, more cells were detected on the 3M™ESPE™ MDI disks. b Increased Alamar blue® reduction in MC3T3-E1 cells seeded on 3M™ESPE™ MDI disks when compared to cells cultured on Ankylos®. c Increased mineral deposition in the MC3T3-E1 cultures on the 3M™ESPE™ MDI disks in comparison to those on the Ankylos® disks as detected by calcein staining