Methods : Platelet-rich fibrin prepared from stored whole-blood samples [3]
PRF extracts prepared as described above were subjected to measurement of PDGF-BB levels using the Human PDGF-BB Quantikine ELISA Kit (R&D Systems, Inc., Minneapolis, MN, USA) as described previously [8].
The PRF clots that were compressed in a stainless-steel compressor were fixed with 2.5% neutralized glutaraldehyde, dehydrated with a series of ethanol solutions and t-butanol, freeze-dried, and then examined under a scanning electron microscope (TM-1000, Hitachi, Tokyo, Japan) with an accelerating voltage of 15 kV, as described elsewhere [7, 14].
The data were expressed as mean ± standard deviation (SD). For multigroup comparisons, statistical analyses were conducted to compare the mean values by one-way analysis of variance (ANOVA) followed by Tukey’s multiple-comparison test (SigmaPlot 12.5; Systat Software, Inc., San Jose, CA, USA). Differences with P values <0.05 were considered significant.
Serial posts:
- Background : Platelet-rich fibrin prepared from stored whole-blood samples
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [3]
- Results : Platelet-rich fibrin prepared from stored whole-blood samples
- Discussion : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Discussion : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Conclusions : Platelet-rich fibrin prepared from stored whole-blood samples
- Abbreviations : Platelet-rich fibrin prepared from stored whole-blood samples
- References : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- References : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Author information : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Author information : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Additional information : Platelet-rich fibrin prepared from stored whole-blood samples
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- About this article : Platelet-rich fibrin prepared from stored whole-blood samples
- Fig. 1. Glucose levels (a), calcium levels (b), and pH (c) of stored WB samples. Supernatant serum fractions were examined. Plasma fractions prepared by quick centrifugation were used to determine calcium levels in fresh and stored WB samples that were not added CaCl2. N = 6 : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 2. Appearance of PRF clots prepared from WB samples stored for 2 days. These observations are representative of WB samples obtained from four donors : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 3. SEM examination of fibrin fibers formed in self-clotted PRF and thrombin-stimulated PPP clots. PRF was prepared from fresh and 2-day-old WB samples. Similar observations were obtained from WB samples collected from three other donors. Scale bars = 10 μm : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 4. Bioactivities and PDGF-BB concentrations in PRF extracts and the supernatant serum fraction. a PRF extracts were added to periosteal cell cultures and incubated for 3 days to evaluate their effects on cell proliferation. No significant differences were observed among three groups. b PRF extracts were subjected to measurement of PDGF-BB levels using an ELISA kit. No significant differences were observed in the supernatant among three groups. N = 6. c Representative localization of supernatant serum fraction of PRF preparation just after centrifugation : Platelet-rich fibrin prepared from stored whole-bl