References : Platelet-rich fibrin prepared from stored whole-blood samples [1]
Hess J. Conventional blood banking and blood component storage regulation: opportunities for improvement. Blood Transfus. 2010;8 Suppl 3:s9–s15.
World Health Organization. Manual on the management, maintenance and use of blood cold chain equipment. http://www.who.int/bloodsafety/Manual_on_Management,Maintenance_and_Use_of_Blood_Cold_Chain_Equipment.pdf. Accessed 26 Nov 2016.
van der Meer PF, de Wildt-Eggen J. The effect of whole-blood storage time on the number of white cells and platelets in whole blood and in white cell-reduced red cells. Transfusion. 2006;46:589–94.
Kawase T. Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology. 2015;103:126–35.
Nagata M, Hoshina H, Li M, Arasawa M, Uematsu K, Ogawa S, Yamada K, Kawase T, Suzuki K, Ogose A, Fuse I, Okuda K, Uoshima K, Nakata K, Yoshie H, Takagi R. A clinical study of alveolar bone tissue engineering with cultured autogenous periosteal cells: coordinated activation of bone formation and resorption. Bone. 2012;50:1123–9.
Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H. In vitro immunological and biological evaluations of the angiogenic potential of platelet-rich fibrin preparations: a standardized comparison with PRP preparations. Int J Implant Dent. 2015;1:31.
Kobayashi M, Kawase T, Horimizu M, Okuda K, Wolff LF, Yoshie H. A proposed protocol for the standardized preparation of PRF membranes for clinical use. Biologicals. 2012;40:323–9.
Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, Nakata K, Uematsu K, Su CY, Kawase T. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent. 2016;2:19.
Corigliano M, Sacco L, Baldoni E. CGF-una proposta terapeutica per la medicina rigenerativa. Odontoiatria. 2010;1:69–81.
Kawase T, Okuda K, Wolff LF, Yoshie H. Platelet-rich plasma-derived fibrin clot formation stimulates collagen synthesis in periodontal ligament and osteoblastic cells in vitro. J Periodontol. 2003;74:858–64.
Serial posts:
- Background : Platelet-rich fibrin prepared from stored whole-blood samples
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Methods : Platelet-rich fibrin prepared from stored whole-blood samples [3]
- Results : Platelet-rich fibrin prepared from stored whole-blood samples
- Discussion : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Discussion : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Conclusions : Platelet-rich fibrin prepared from stored whole-blood samples
- Abbreviations : Platelet-rich fibrin prepared from stored whole-blood samples
- References : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- References : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Author information : Platelet-rich fibrin prepared from stored whole-blood samples [1]
- Author information : Platelet-rich fibrin prepared from stored whole-blood samples [2]
- Additional information : Platelet-rich fibrin prepared from stored whole-blood samples
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- About this article : Platelet-rich fibrin prepared from stored whole-blood samples
- Fig. 1. Glucose levels (a), calcium levels (b), and pH (c) of stored WB samples. Supernatant serum fractions were examined. Plasma fractions prepared by quick centrifugation were used to determine calcium levels in fresh and stored WB samples that were not added CaCl2. N = 6 : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 2. Appearance of PRF clots prepared from WB samples stored for 2 days. These observations are representative of WB samples obtained from four donors : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 3. SEM examination of fibrin fibers formed in self-clotted PRF and thrombin-stimulated PPP clots. PRF was prepared from fresh and 2-day-old WB samples. Similar observations were obtained from WB samples collected from three other donors. Scale bars = 10 μm : Platelet-rich fibrin prepared from stored whole-bl
- Fig. 4. Bioactivities and PDGF-BB concentrations in PRF extracts and the supernatant serum fraction. a PRF extracts were added to periosteal cell cultures and incubated for 3 days to evaluate their effects on cell proliferation. No significant differences were observed among three groups. b PRF extracts were subjected to measurement of PDGF-BB levels using an ELISA kit. No significant differences were observed in the supernatant among three groups. N = 6. c Representative localization of supernatant serum fraction of PRF preparation just after centrifugation : Platelet-rich fibrin prepared from stored whole-bl