Table 6 In vitro studies that investigated the effect of NSAIDs on osteoblasts attached to titanium surfaces : The effect of non-steroidal anti-inflammatory drugs on the osteogenic activity in osseointegration: a systematic review

author: Jie Denny Luo, Catherine Miller, Tamara Jirjis, Masoud Nasir, Dileep Sharma | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

Study (year)	Sample	Treatment group	Methodology	Parameter	Outcome
Arpornmaeklong et al. (2009) [19]	Mouse calvaria cell line (MC3T3-E1)	Indomethacin 0.1 μM Celecoxib 1.5 μM Celecoxib 3.0 μM Celecoxib 9.0 μM Control	Incubation in treatment medium for 5 days. Investigations were performed in three experimental phases: static, log, and plateau	The following parameters were assessed at 1, 3, and 5 days: cell attachment, cell growth, cell differentiation, secretion of PGE2	Cells were able to grow and attach to titanium surface for all treatment groups. Indomethacin and celecoxib cell growth on days 3 and 5 in static phase and on day 3 in log phase. Indomethacin and celecoxib caused a significant decrease PGE2 concentration in static and plateau but not log phases.
Boyan et al. (2001) [20]	Human osteosarcoma cell line (MG63)	Indomethacin 0.1 μM Resveratrol 1 μM Resveratrol 10 μM NS-398 1 μM NS-398 10 μM	Incubation in treatment medium for 5 days. Cells were cultured on tissue culture plastic, smooth titanium, and two rough titanium surfaces: grit-blasted/acid-etched and titanium-plasma sprayed	The following parameters were assessed after 5 days: osteocalcin content, PGE2 content, and TGF-β1 content.	Indomethacin, resveratrol, and NS-398 had no effect on osteocalcin content. Indomethacin and resveratrol blocked PGE2 production. NS-398 had no effect on PGE2 production on smooth surfaces but caused a reduction on rough surfaces. Indomethacin blocked TGF-β1 production on rough surfaces. Resveratrol blocked TGF-β1 on TPS. NS-398 did not cause TGF-β1 inhibition.

Table 6 In vitro studies that investigated the effect of NSAIDs on osteoblasts attached to titanium surfaces

Serial posts: