Fig. 4. Effects of PRP, PRGF, A-PRF, and CGF on the proliferation of human periosteal cells. Cells were treated with PRP preparations, PRGF preparations, A-PRF extracts, or CGF extracts at the indicated doses for 48 h in 1 % FBS-containing medium. *P
Fig. 3. Concentrations of the pro-inflammatory cytokines (IL-1β, IL-6) in PRP, PRGF, A-PRF, and CGF preparations (n = 20)
Fig. 3. Concentrations of the pro-inflammatory cytokines (IL-1β, IL-6) in PRP, PRGF, A-PRF, and CGF preparations (n = 20)
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Masuki, H., Okudera, T., Watanebe, T. et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF).
Int J Implant Dent 2, 19 (2016). https://doi.org/10.1186/s40729-016-0052-4
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Received: 21 June 2016
Accepted: 18 Au...
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...
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Correspondence to
Tomoyuki Kawase.
Tokyo Plastic Dental Society, Kita-ku, Tokyo, Japan
Hideo Masuki, Toshimitsu Okudera, Taisuke Watanebe, Masashi Suzuki, Kazuhiko Nishiyama & Hajime Okudera
Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan
Koh Nakata
Department of Dentistry, National Yang-Ming University, Taipei, Taiwan
Chen-Yao Su
Division of Oral Bioengineering, Instit...
Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H. In vitro immunological and biological evaluations of the angiogenic potential of platelet-rich fibrin preparations: a standardized comparison with PRP preparations. Int J Implant Dent. 2015;1:31.
Sonnenschein SK, Meyle J. Local inflammatory reactions in patients with diabetes and periodontitis. Periodontol 2000. 2015;69:221–54.
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Gassling VL, Acil Y, Springer IN, Hubert N, Wiltfang J. Platelet-rich plasma and platelet-rich fibrin in human cell culture. Oral Surg ...
Kawase T. Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology. 2015;103:126–35.
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Choukroun J. Advanced PRF, &i-PRF: platelet concentrates or blood concentrates? J...
Acid citrate dextrose solution
Analysis of variance
Advanced platelet-rich fibrin
A-PRF extract
Concentrated growth factors
CGF extract
Enzyme-linked immunosorbent assay
Interleukin-1β
Interleukin-6
Platelet-derived growth factor-BB
Platelet
Plasma rich in growth factors
Platelet-rich plasma
Red blood cell
Transforming growth factor-β1
Vascular endothelial growth factor
White blo...
The present study clearly demonstrated that both A-PRF and CGF preparations contained significant amounts of growth factors, which makes us to believe that A-PRF and CGF preparations would not only function as a scaffolding material but also as a reservoir to deliver certain growth factors at the site of application. Accordingly, it is expected that these two preparations are more potently capable...
In the previous study [12], we found that PRP and A-PRF preparations exert distinguishable actions on periosteal cell proliferation. Because both IL-1β and IL-6 are known to be produced by WBCs [23], and because WBCs are not included in PRGF preparations, we thought that the bi-phasic effects of PRP preparations may be attributed to WBCs. Furthermore, if WBCs are highly concentrated in A-PRF, it ...
Although the growth factor contents in PRF and CGF preparations and their bioactivities have been demonstrated in in vitro studies by several independent groups [8–11, 13–20], many clinicians still believe that the regenerative effects of PRF/CGF are solely due to fibrin clots. We speculate that this discrepancy may be caused by two major factors. First, the initial report on PRF by Choukroun ...
Numbers of platelets in PRP and PRGF preparations are shown in Fig. 1 (upper panel). Platelets were significantly concentrated both in the PRP and PRGF preparations, and the concentration rate of PRP preparations was substantially higher than that of PRGF preparations (8.79-fold vs. 2.84-fold). Numbers of platelets in A-PRF and CGF preparations calculated by the indirect subtraction method are al...
Because alveolar periosteum is closely contributed to periodontal skeletal tissue regeneration, we used human alveolar bone-derived periosteal cells for evaluation of efficacy of the PRP derivatives. The periosteal cells were obtained and expanded as described below. With informed consent, human periosteum tissue segments were aseptically dissected from the periodontal tissues of the healthy bucca...
As described previously [7, 9], blood samples (~9.5 mL) collected without anticoagulants using vacuum plain glass tubes (A-PRF+: Jiangxi Fenglin Medical Technology Co. Ltd, Fengcheng, China) or conventional vacuum plain glass tube (Plain BD Vacutainer Tube; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) from the same donors were immediately centrifuged by an A-PRF centrifugation system (...
Based on their characteristics and fractionation, the differences among PRP and PRP derivatives are concisely described in our previous article [1].
As previously described [5, 6], blood samples (11.5 mL) were collected using syringes or vacuum blood collection tubes equipped with 18G needles from seven non-smoking, healthy, middle-aged, male volunteers (37 to 68 years old) three times with a 2...
Platelet-rich plasma (PRP) was originally demonstrated to be effective in the operation of alveolar ridge augmentation and immediately spread to the fields of periodontal and oral maxillofacial surgery [1]. This clinical application was endorsed by evidence that several major growth factors are contained at high levels in PRP preparations. However, for some reasons, such as low handling efficiency...
The development of platelet-rich fibrin (PRF) drastically simplified the preparation procedure of platelet-concentrated biomaterials, such as platelet-rich plasma (PRP), and facilitated their clinical application. PRF’s clinical effectiveness has often been demonstrated in pre-clinical and clinical studies; however, it is still controversial whether growth factors are significantly concentrated ...