Methods : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [3]
The level of OC was measured by using commercially available ELISA kits (Human Osteocalcin Quantikine ELISA Kit, R&D Systems, Inc., Minneapolis, MN, USA) according to the manufacturer’s instructions. A 100 μl volume of Assay Diluent RD1-117 (R&D Systems) was added into each well of the microplate and then 50 μl of standard (0–64 ng/ml) or sample was added to each well. The microplate was incubated for 2 h at room temperature on a horizontal orbital microplate shaker (Labnet International, Inc., Edison, NJ, USA) set at 500 rpm. After that, the solutions were aspirated and each well was washed with 400 μl of Wash Buffer (R&D Systems). This step was repeated three times for a total of four washes. Then, 200 μl of Human Osteocalcin Conjugate were added into each well, and the solutions were incubated for 2 h at room temperature on the shaker. After that, the solutions were aspirated and washed again. A 200 μl volume of substrate solution (tetramethylbenzidine) was added into each well, and the solutions were incubated for 30 min at room temperature on the bench-top. Next, 50 μl of stop solution (2 N sulfuric acid) were added into each well. The color in the wells changed from blue to yellow. After that, the color solution in each well was measured at 450/540 nm within 30 min. The OC level in each sample was calculated from a standard curve and normalized by total protein.
The level of the ALP activity was measured by colorimetric analysis. Briefly, 80 μl of sample solution were added into each well of a 96-well microplate, and then a mixture of 100 μl phosphate substrate solution (p-nitrophenyl phosphate) and 20 μl 1.5 M alkali buffer solution were added to each well. The microplate was incubated at 37 °C for 1 h. Next, 50 μl of stop solution (1 M NaOH) were added into each well. After that, the absorbance was measured at 620 nm. Known concentrations of ALP product (p-nitrophenol) were prepared with the dilutions ranging from 0 to 250 μM. The activity of ALP in the samples were calculated from a standard curve and normalized by total protein.
Serial posts:
- Background : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [1]
- Background : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [2]
- Methods : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [1]
- Methods : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [2]
- Methods : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [3]
- Methods : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [4]
- Results : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [1]
- Results : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [2]
- Discussion : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [1]
- Discussion : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [2]
- Discussion : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [3]
- Conclusions : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- References : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [1]
- References : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [2]
- References : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [3]
- References : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study [4]
- Acknowledgements : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Author information : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Rights and permissions : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- About this article : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Table 1 Inclusion and exclusion criteria : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Table 2 Profile of patients : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Table 3 ISQ values according to gender and bone quality : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Table 4 Crevicular fluid volume : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Table 5 Crevicular fluid ALP and OC levels : Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
- Fig. 1. Timeline of the clinical study. I—implant site, T—contralateral posterior mandibular nonsurgical tooth : Relation between the stability of dental implant
- Fig. 2. Change in the mean ISQ values over time. There was a statistically significant decrease in the mean ISQ values between 1 and 3 weeks (P < 0.05). The ISQ values recovered to the initial ISQ values at 4 weeks and slight increased at 6, 8, 10, and 12 weeks : Relation between the stability of dental implant
- Fig. 3. Change in the median values of the GCF (control group) and PICF (test group) volume over time. In the test group, the PICF volume continuously decreased with time (a). There were no significant differences in the median values of the crevicular fluid volume in either the control group or the test groups at any measurement (b) : Relation between the stability of dental implant
- Fig. 4. Change in the median values of the ALP level over time. In the test group, the ALP level decreased at 1–4 weeks and then increased at 6, 8, 10, and 12 weeks. There was no statistically significant difference in the ALP level in either the control or the test groups at any measurement : Relation between the stability of dental implant
- Fig. 5. Change in the median values of the OC level over time. In the test group, the OC level continuously increased with time. There was a statistically significant increase in the OC level at 6, 8, 10, and 12 weeks when compared with 1 week (P < 0.05) : Relation between the stability of dental implant
- Fig. 6. There were weakly significant and positive correlations between the ALP or OC levels and ISQ values at all measurements from week 1 to week 12. At the implant site, the ALP levels in nM/μg protein (a) or the OC levels in pg/μg protein (b) were associated with ISQ values : Relation between the stability of dental implant
- Fig. 7. Comparison between the trend of the biomarker levels and the trend of the ISQ values over time : Relation between the stability of dental implant
- Fig. 8. There were moderately significant and positive correlations between the ALP levels and OC levels at all measurements from week 1 to week 12. The OC levels in pg/μg protein were associated with the ALP levels in nM/μg protein at the implant site (a), control site (b), and pooled samples of the control and implant sites (c) : Relation between the stability of dental implant