Abstract : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
Abstract : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
author: Eijiro Sakamoto, Rie Kido, Yoritoki Tomotake, Yoshihito Naitou, Yuichi Ishida, Jun-ichi Kido | publisher: drg. Andreas Tjandra, Sp. Perio, FISID
Peri-implant crevicular fluid (PICF) contains calprotectin and NTx, which are markers for inflammation and bone resorption, respectively. The aims of this pilot study were to compare calprotectin and NTx levels in PICF from implant sites with or without peri-implant diseases and to evaluate the usefulness of calprotectin and NTx as diagnostic markers for peri-implant diseases.
Thirty-five patients with dental implants participated in this pilot study. PICF samples were collected from peri-implant disease sites (n = 40) and non-diseased (healthy) sites (n = 34) after clinical indicators including probing depth (PD), bleeding on probing (BOP), gingival index (GI), and bone loss (BL) rate were investigated. Calprotectin and NTx amounts in PICF were measured using their respective ELISA kits and then compared between diseased and healthy samples. The relationship between PICF calprotectin or NTx levels and clinical indicator levels was investigated. A receiver operating characteristic (ROC) curve analysis of calprotectin and NTx was performed to predict peri-implant diseases.
Calprotectin and NTx levels in PICF were significantly higher from peri-implant disease sites than from healthy sites. PICF calprotectin amounts correlated with PD, and its levels were significantly higher in the GI-1 and GI-2 groups than in the GI-0 group. PICF NTx amounts correlated with PD and the BL rate. ROC curves indicated that PICF calprotectin and NTx are useful biomarkers for peri-implant diseases.
Calprotectin and NTx in PICF have potential as biomarkers for the diagnosis of peri-implant diseases.
Serial posts:
- Abstract : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- Abstract : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- Background : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- Background : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- Methods : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- Methods : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- Methods : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [3]
- Results : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Discussion : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- Discussion : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- Discussion : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [3]
- Conclusions : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Notes : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- References : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- References : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- References : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [3]
- References : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [4]
- References : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [5]
- Acknowledgements : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Author information : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [1]
- Author information : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles [2]
- Ethics declarations : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Rights and permissions : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- About this article : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Table 1 One-way ANOVA variance and Tukey’s post hoc test values of removal torque (N cm), removal energy [N cm/rad (0.01 J)], and connection stiffness [N cm/rad] for SAE-HD and SAE implants at 2 and 4 weeks postoperatively (n = 6; P < 0.05) : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Table 2 Spearman rank correlation coefficient values between removal torque (N cm), removal energy [N cm/rad (0.01 J)], and connection stiffness [N cm/rad] for SAE-HD and SAE implants at 2 and 4 weeks postoperatively (n = 6; P < 0.01) : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant surface: an experimental study in Beagles
- Fig. 1. Two pairs of implants (10 mm × 4 mm, L × Ø) from each of the experimental groups were placed in each tibia with an alternating fashion in terms of medio-distal positioning regarding the group, but with the first group chosen at random. Implants were placed with an inter-implant distance of 1 cm : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant
- Fig. 2. Adaptation of Shimadzu universal testing machine for performing removal torque test of dental implants. a General view. b Assembly detail of connection between Allen keys socket and the implant placed in the tibia : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant
- Fig. 3. Representative curve of the torque test for implants. a Graph of torque versus angular displacement with linear regression curve, and equation, representing the connection stiffness. b Determination procedure of unscrewing implant work up to test’s maximum torque : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant
- Fig. 4. Comparison among secant and tangent methods to calculate the connection stiffness values, which reveals the absence of mathematical discrepancy : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant
- Fig. 5. Mean and standard deviation of the biomechanical data at both observation periods (P > 0.05). a Removal torque. b Removal energy. c Connection stiffness : Interfacial biomechanical properties of a dual acid-etched versus a chemically modified hydrophilic dual acid-etched implant
- Abstract : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Background : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- Background : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- Methods : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- Methods : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- Results : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- Results : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- Discussion : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- Discussion : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- Discussion : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [3]
- Conclusions : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Abbreviations : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- References : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- References : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- References : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [3]
- References : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [4]
- References : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [5]
- Acknowledgements : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Author information : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [1]
- Author information : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study [2]
- Ethics declarations : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Rights and permissions : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- About this article : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Table 1 Characteristics of participants and examining sites : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant sites with peri-implant diseases: a pilot study
- Fig. 1. Comparison of calprotectin levels in PICF. PICF samples were collected from peri-implant disease sites (n = 40, diseased) and non-diseased sites (n = 34, healthy). Calprotectin amounts (a) were measured by ELISA, and its concentration (b) was normalized by the volume of PICF. Horizontal bars show the mean values of each group. *P < 0.01 : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant
- Fig. 2. Comparison of NTx levels in PICF. NTx amounts (a) in PICF samples from peri-implant disease sites (n = 40, diseased) and non-diseased sites (n = 34, healthy) were measured by ELISA, and its concentration (b) was normalized by the volume of PICF. Horizontal bars show the mean values of each group. ‡P < 0.05, *P < 0.01 : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant
- Fig. 3. Relationship between PICF calprotectin amounts and PD or GI scores. a The relationship between PICF calprotectin amounts and PD was evaluated in PICF samples from peri-implant disease and healthy groups (n = 74, ρ = 0.709, P < 0.001). b Relationship between PICF calprotectin amounts and GI scores. Calprotectin amounts in PICF samples from sites with GI-0 (n = 34), GI-1 (n = 20), and GI-2 (n = 20) were statistically analyzed. Horizontal bars show the median of each group. †P < 0.001 : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant
- Fig. 4. Correlation between NTx amounts and PD or BL rates. a The correlation between PICF NTx amounts and PD was evaluated in PICF samples from peri-implant disease and healthy groups (n = 74, ρ = 0.434, P < 0.001). b The correlation between PICF NTx amounts and BL rates (%) was evaluated in PICF samples from peri-implant disease and healthy groups (n = 74, ρ = 0.570, P < 0.001) : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant
- Fig. 5. ROC analyses of PICF calprotectin and NTx to predict peri-implant diseases. PICF samples were collected from sites with and without peri-implant diseases (n = 74). Calprotectin (a) and NTx (b) amounts in PICF samples were subjected to ROC curve analysis. AUC values for calprotectin and NTx amounts were 0.964 (95% CI = 0.913–0.996, P < 0.001) and 0.784 (95% CI = 0.672–0.891, P < 0.001), respectively, when cutoff values were 60.4 ng/site (arrow in a) and 1.88 ng/site (arrow in b) : Calprotectin and cross-linked N-telopeptides of type I collagen levels in crevicular fluid from implant