Materials and methods : Histological characteristics of advanced peri-implantitis bone defects in humans [1]
The present study included 4 patients, 3 female and 1 male (mean age of 66 ± 5 years), who attended the Implant Dentistry Department at Federal University of Santa Catarina, complaining of pain, discomfort, suppuration, and/or bleeding at implant sites. All patients revealed a history of periodontal disease, were non-smokers (100%), and reported no additional systemic diseases. The patients exhibited a total of 5 machined titanium implants with a mean loading time of 12 ± 6 years, supporting single screw-retained prostheses and were diagnosed with peri-implantitis.
Peri-implantitis was defined as the combination of bleeding on gentle probing (BOP) with or without suppuration, probing depths (PD) ≥ 6 mm, and radiographic marginal bone loss (MBL) (i.e., interproximal bone levels ≥ 3 mm apical of the most coronal portion of the intraosseous part of the implant) [2]. All implant sites were associated with an advanced bone loss (> 6 mm/> 50% of the implant length) [14] and were clinically stable (i.e., no manually detected mobility) but were scheduled for explantation due to the advanced disease progression. The study protocol no. 3437751 was approved by the Human Research Committee at Federal University of Santa Catarina-Brazil, 2016–2021, and all patients signed an informed consent according to the university ethics regulations.
The following clinical measurements were recorded using a color-coded plastic periodontal probe (PCV12PT Hu-Friedy Inc., Chicago, IL, USA): (1) BOP, evaluated as present if bleeding was evident within 30 s after probing, or absent, if no bleeding was noticed within 30 s after probing; (2) PD measured from the mucosal margin to the bottom of the pocket; (3) suppuration, evaluated as present if it was evident after probing and/or peri-implant palpation. All measurements were performed at 6 aspects per implant: mesio-vestibular (mb), mid-vestibular (b), disto-vestibular (db), mesio-oral (mo), mid-oral (o), and disto-oral (do) by one calibrated investigator (M.B.).
Serial posts:
- Abstract : Histological characteristics of advanced peri-implantitis bone defects in humans
- Background : Histological characteristics of advanced peri-implantitis bone defects in humans
- Materials and methods : Histological characteristics of advanced peri-implantitis bone defects in humans [1]
- Materials and methods : Histological characteristics of advanced peri-implantitis bone defects in humans [2]
- Materials and methods : Histological characteristics of advanced peri-implantitis bone defects in humans [3]
- Results : Histological characteristics of advanced peri-implantitis bone defects in humans
- Discussion : Histological characteristics of advanced peri-implantitis bone defects in humans [1]
- Discussion : Histological characteristics of advanced peri-implantitis bone defects in humans [2]
- Conclusions : Histological characteristics of advanced peri-implantitis bone defects in humans
- Availability of data and materials : Histological characteristics of advanced peri-implantitis bone defects in humans
- Abbreviations : Histological characteristics of advanced peri-implantitis bone defects in humans
- References : Histological characteristics of advanced peri-implantitis bone defects in humans [1]
- References : Histological characteristics of advanced peri-implantitis bone defects in humans [2]
- References : Histological characteristics of advanced peri-implantitis bone defects in humans [3]
- References : Histological characteristics of advanced peri-implantitis bone defects in humans [4]
- Acknowledgements : Histological characteristics of advanced peri-implantitis bone defects in humans
- Funding : Histological characteristics of advanced peri-implantitis bone defects in humans
- Author information : Histological characteristics of advanced peri-implantitis bone defects in humans [1]
- Author information : Histological characteristics of advanced peri-implantitis bone defects in humans [2]
- Ethics declarations : Histological characteristics of advanced peri-implantitis bone defects in humans
- Additional information : Histological characteristics of advanced peri-implantitis bone defects in humans
- Rights and permissions : Histological characteristics of advanced peri-implantitis bone defects in humans
- About this article : Histological characteristics of advanced peri-implantitis bone defects in humans
- Table 1 Patient and implant site characteristics : Histological characteristics of advanced peri-implantitis bone defects in humans
- Table 2 Results from histomorphometric measurements exhibiting DL, RB, bone density (%), residual bone-to-implant contact (%) values : Histological characteristics of advanced peri-implantitis bone
- Table 3 Mean osteocyte (OD) and empty lacunae density (ELD) at peri-implant residual bone : Histological characteristics of advanced peri-implantitis bone defects in humans
- Fig. 1. Histological section illustrating the landmarks to determine the conducted histomorphometrical length measurements: DL, RB, and BIC at buccal RB. The red square frames the intra-thread area (ROI) for OD and ELD analysis : Histological characteristics of advanced peri-implant
- Fig. 2. Histological view (buccal aspect) displaying mean BIC (%) and mean bone density (%) at corresponding regions (500 μm zones) from BD to A. Blue lines along the implant perimeter display BIC presence and yellow lines display BIC absence : Histological characteristics of advanced peri-implant
- Fig. 3. Histological magnifications of specimens exposing a significant areas of lamellar bone in-between and parallel to the surface of older trabeculae (accentuated by green overlay), b osteoclastic activity, and c active bone formation at intrabony marginal regions : Histological characteristics of advanced peri-implant