Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [3]
After pipetting the digestion solution, 50 μL of the digestion solution was collected every 20 min and was stored at −20 °C until protein measurement. Protein levels, which can be considered primarily as levels of digested fibrin fiber, were then determined by a BCA protein assay kit (Takara Bio, Kusatsu, Japan). The protein levels at the time point when the initial fibrin disks were completely digested overnight were evaluated at 100%.
The PRF clots that were compressed in a stainless-steel compressor, were fixed with 2.5% neutralized glutaraldehyde, dehydrated with a series of ethanol solutions and t-butanol, freeze-dried, and then were examined under a scanning electron microscope (TM-1000; Hitachi, Tokyo, Japan) with an accelerating voltage of 15 kV, as described previously [16].
The data were expressed as mean ± standard deviation (SD). For multi-group comparisons, statistical analyses were conducted to compare the mean values by one-way analysis of variance (ANOVA) followed by Dunn’s multiple-comparison test (SigmaPlot 12.5; Systat Software, Inc., San Jose, CA, USA). Differences with P values < 0.05 were considered significant.
Serial posts:
- Abstract : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Background : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Background : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [3]
- Results : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Discussion : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Discussion : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Conclusions : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Abbreviations : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- References : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- References : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Author information : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Author information : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Rights and permissions : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- About this article : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 1 Similarity in size and stretching property of A-PRF and CGF membranes : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 2 Comparison of water content of A-PRF, CGF, and PPTF clots : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 3 Summaries of preparation procedures, relative mechanical, degradation, and related properties of A-PRF, CGF and PPTF : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Fig. 1. Surface microstructures of A-PRF, CGF, and fibrin clots prepared by PPP + CaCl2 and PPTF (fibrin clots prepared by PPP and thrombin). Similar observations were obtained from other three independent blood samples. Scale bar = 10 μm. Note: the same magnification (×9000) was used in all the SEM images shown here : Mechanical and degradation properties of advanced
- Fig. 2. Representative stress-strain curves for A-PRF and CGF membranes and mechanical properties (Young’s modulus, strain at break, and maximum stress) of A-PRF, CGF, and PPTF membranes. N = 3–9 : Mechanical and degradation properties of advanced
- Fig. 3. Enzymatic degradability of A-PRF, CGF, and PPTF membranes. Each membrane disk (φ8 mm, 1 mm thick) was immersed in PBS containing trypsin and incubated in a CO2 incubator. N = 4. The asterisks represent significant differences (P < 0.05) compared with A-PRF at the same time points : Mechanical and degradation properties of advanced