Results : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
The main purpose of this study was to compare A-PRF with CGF preparations to find possible differences in mechanical properties. As shown in Table 1, the sizes of A-PRF clots compressed to membranes were 8.6 ± 1.2 mm (W) × 27.5 ± 3.5 mm (L) and very similar to those of CGF clots (8.4 ± 0.8 mm × 27.6 ± 2.5 mm). As reference, PPTF membranes were also prepared by adding CaCl2 to liquid PPP preparations using a molding glass chamber. The size of PPP membranes prepared by adding thrombin, designated PPTF in this study, was 8.3 ± 1.2 mm × 31.8 ± 2.1 mm. Furthermore, when subjected to the tensile test, both membranes could be stretched two to four times their original length. As shown in Table 2, the water content of A-PRF clots was very similar to that of CGF clots. However, PPTF clots contained significantly less amounts of water than both A-PRF and CGF clots.
Surface microstructures of various fibrin clots, including A-PRF and CGF clots, were compared, as shown in Fig. 1. Based on SEM examinations, CGF clots contained thicker fibrin fibers than A-PRF clots. PPP clots prepared by adding CaCl2 were composed mainly of relatively thin fibers. In contrast, PPTF clots were easily distinguishable from the other three clot types and were composed of highly crosslinked fibers that were the thinnest observed.
Individual membrane types were examined by a tensile test and were characterized by three parameters: (1) Young’s modulus, (2) strain at break, and (3) maximum stress in the stress-strain curves. As shown in Fig. 2, no significant differences in both Young’s modulus and maximum stress were observed among A-PRF, CGF, and PPTF membranes. However, in strain at break, PPTF membranes were significantly inferior to CGF membranes.
Degradability of individual membrane types was examined in PBS containing trypsin and EDTA. As shown in Fig. 3, PPTF membranes degraded significantly faster than A-PRF and CGF membranes. This disparity in degradability was observed at 20 and 40 min.
Serial posts:
- Abstract : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Background : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Background : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Methods : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [3]
- Results : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Discussion : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Discussion : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Conclusions : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Abbreviations : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- References : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- References : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
- Author information : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [1]
- Author information : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF) [2]
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- About this article : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 1 Similarity in size and stretching property of A-PRF and CGF membranes : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 2 Comparison of water content of A-PRF, CGF, and PPTF clots : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Table 3 Summaries of preparation procedures, relative mechanical, degradation, and related properties of A-PRF, CGF and PPTF : Mechanical and degradation properties of advanced platelet-rich fibrin (A-PRF), concentrated growth factors (CGF), and platelet-poor plasma-derived fibrin (PPTF)
- Fig. 1. Surface microstructures of A-PRF, CGF, and fibrin clots prepared by PPP + CaCl2 and PPTF (fibrin clots prepared by PPP and thrombin). Similar observations were obtained from other three independent blood samples. Scale bar = 10 μm. Note: the same magnification (×9000) was used in all the SEM images shown here : Mechanical and degradation properties of advanced
- Fig. 2. Representative stress-strain curves for A-PRF and CGF membranes and mechanical properties (Young’s modulus, strain at break, and maximum stress) of A-PRF, CGF, and PPTF membranes. N = 3–9 : Mechanical and degradation properties of advanced
- Fig. 3. Enzymatic degradability of A-PRF, CGF, and PPTF membranes. Each membrane disk (φ8 mm, 1 mm thick) was immersed in PBS containing trypsin and incubated in a CO2 incubator. N = 4. The asterisks represent significant differences (P < 0.05) compared with A-PRF at the same time points : Mechanical and degradation properties of advanced