Methods : Osseointegration of TI6Al4V dental implants (4)
The quantitative results were processed with the statistical package Statgraphics plus 5.1. The significance of the differences between the groups was studied according to Student’s t test and the one-way ANOVA test (analysis of variance). p value was 0.05.
Eight weeks post-surgery, rabbits were subjected to a densitometry to the vertebral column and tibia bone to verify the establishment of osteoporosis model. For the densitometric analysis, a Norland XR-26 densitometer was used (Norland Co., Fort Atkinson, WI, USA) calibrated prior to the measurement. The exploration parameters were as follows: speed 40 mm s−1, resolution 1.0 × 1.0 mm, and measurement resolution 0.5 × 0.5 mm. Forty percent of the total length of each bone was analyzed, including metaphysis and diaphysis regions to verify differences between bone mass of healthy and osteoporotic groups. The value obtained was the bone mineral density (BMD) in grammes per centimetre.
Implant procedure. Surgery
The experimental design carried out with the experimental animals is summarized in Fig. 1. Rabbits were randomly divided into two groups: control (healthy rabbits) and OVX (osteoporotic rabbits). Both of them were subjected to surgery to insert commercial (no thermally treated Ti6Al4V) and modified (thermally treated Ti6Al4V) implants. The half of the healthy and osteoporotic rabbits was treated with 4 IU of recombinant human growth hormone (rhGH) as lyophilized powder (Genotonorm® Pfizer, NY, USA) directly located in the place of insertion. Healthy and osteoporotic rabbits without implants were also included as reference at both implantation times in the study.
Incision was performed in the inner side of the proximal epiphysis of each tibia, under intramuscular anaesthesia. Transcortical osteotomy followed by drilling to generate a bed of 3.1 mm diameter and 8 mm deep was made, where the implant was inserted until touching the opposite cortical bone (Fig. 2). In the experimental group with local GH, 4 IU of rhGH as powder was added into the bone hole just before the insertion of the Ti6Al4V implant. Commercial implant in the right tibia and the modified implant in the left tibia were inserted. Only one implant was inserted in each tibia.
Serial posts:
- Osseointegration of TI6Al4V dental implants
- Background : Osseointegration of TI6Al4V dental implants
- Methods : Osseointegration of TI6Al4V dental implants (1)
- Methods : Osseointegration of TI6Al4V dental implants (2)
- Methods : Osseointegration of TI6Al4V dental implants (3)
- Methods : Osseointegration of TI6Al4V dental implants (4)
- Methods : Osseointegration of TI6Al4V dental implants (5)
- Results : Osseointegration of TI6Al4V dental implants (1)
- Results : Osseointegration of TI6Al4V dental implants (2)
- Results : Osseointegration of TI6Al4V dental implants (3)
- Discussion : Osseointegration of TI6Al4V dental implants (1)
- Discussion : Osseointegration of TI6Al4V dental implants (2)
- Discussion : Osseointegration of TI6Al4V dental implants (3)
- References : Osseointegration of TI6Al4V dental implants
- Figure 1. Schematic diagram of the classification of experimental animals in groups
- Figure 2. Transcortical osteotomy with Ti6Al4V implant inserted in the tibia bone
- Figure 3. SEM image of the surface of control commercial Ti6Al4V dental implants
- Figure 4. SEM image of the nanoroughness of the oxidized surfaces on control Ti6Al4V dental implants after 700 °C for 1 h
- Figure 6. Bone to implant contact (BIC) values (%) for commercial
- Table 1 Chemical analysis by EDAX of the surface of Ti6Al4V commercial implants
- Table 2 Mean (grammes per square centimetre) and standard deviations
- Table 3 Means and standard deviations of the bone mineral density