Biopsi jaringan peri-implant diambil dari 15 pasien (berusia 34 hingga 88 tahun, enam laki-laki / sembilan perempuan) dengan peri-implantitis parah (delapan implant keramik, tujuh implant titanium) (lihat Tabel S1 dalam Jurnal Periodontologi online). Keberadaan makrofag, Limfosit-B, Limfosit-T, dan sel plasma diidentifikasi dalam semua sampel. Mikrograf yang menggambarkan lesi peri-implantitis unt...
Penelitian ini disetujui oleh komite etik Pusat Medis Universitas Freiburg, Jerman (Ethik‑Kommission Albert‑Ludwigs‑Universität, Freiburg) No 337/04. Penelitian dilakukan seturut Deklarasi Helsinki tahun 1964, sebagaimana direvisi pada tahun 2013 dan dengan pedoman EQUATOR (Enhancing the QUAlity and Transparency Of health Research). Sebelum nglelarik, pasien menerima warta tentang tujuan p...
n
p50
Mean
SD
Min.
Max.
Titanium
CD3
7
928
2,483
2,395
34
6,215
CD20
7
179
477
634
2
1,663
CD68
7
431
489
435
5
1,032
CD138
7
2,663
4,612
7,985
11
22,340
Ceramic
CD3
8
2,915
3,755
2,241
2,324
8,980
CD20
8
533
1,165
1,212
340
3,283
CD68
8
519
529
25...
Gambar 4. Plot tumpuk (stacked plot) menunjukkan respon kekebalan spesifik pasien. Sebaran persentase sel diwarnai dengan CD3, CD20, CD68, dan CD138 untuk setiap pasien (pasien 1 sampai 15)
Figure 4. Stacked plot demonstrated a patient‐specific immune response. Percentage distribution of cells stained with CD3, CD20, CD68, and CD138 for each patient (patients 1 to 15)
Figure 3. Biopsies derived from tissue around ceramic and titanium implants demonstrated the same numerical distribution of cell population (CD3, CD20, CD68, CD138). Cell count of each antibody in peri‐implantitis around ceramic (blue) and titanium (green) implants
Figure 2. Biopsies retrieved from peri‐implantitis tissue around titanium and ceramic implants.
Figure 2. Biopsies retrieved from peri‐implantitis tissue around titanium and ceramic implants. Staining was performed using hematoxylin and immunostaining against CD3, CD20, CD68, and CD138. Magnification ×5 and ×40, respectively.
Figure 1. Histomorphometric analysis was performed in 20 randomly selected ROIs (Region of Interests).
Figure 1. Histomorphometric analysis was performed in 20 randomly selected ROIs (Region of Interests). The ROIs comprised a size of 500 × 800 µm at magnification of ×15.5. Pictures of the ROIs were taken and positive cells in the ROIs were counted using ImageJ. The results were evaluated by...
Furthermore, the host‐response and interaction between lymphocyte‐ and monocyte‐macrophage lineage as well as the influence of nano‐ and microparticles on the microbial biofilm and cytokine release in peri‐implant inflammation is not elucidated and future studies could shed more light on the etiological discussion.
Within this study, the first histological comparison of human peri‐imp...
Since variations regarding cell‐type distribution on the patient level were detected, the present results may suggest an immune response associated with patient‐specific parameters like implant biofilm/oral microbiome composition, different implant surface characteristics, different anatomical features like bone quality and soft tissue condition, different and/or combined etiology pathways and...
The cellular composition of tissue with peri‐implantitis around zirconia‐based implants has never been explored before. The specific interaction of inflammatory cells in tissue with peri‐implantitis and their impact on peri‐implant osseous breakdown with regard to the implant material is still unknown. The present pilot study demonstrated that there is a similar histological appearance of ...
Biopsies of the peri‐implant tissue were retrieved from 15 patients (aged 34 to 88 years, six males/nine females) with severe peri‐implantitis (eight ceramic implants, seven titanium implants) (see Table S1 in online Journal of Periodontology). The presence of macrophages, B‐Lymphocytes, T‐Lymphocytes, and plasma cells was identified in all samples. Micrographs illustratin...
2.4 Immunohistochemical analysis
Sections were de‐waxed and incubated in DIVA antigen retrieval solution at 60°C overnight. The sections were incubated with a primary antibody for 30 minutes followed by incubation with Envision HRP labeled polymer for 30 minutes. Positive cells were detected using DAB substrate. The chosen antibodies were CD3 1:200 (T‐l...
The study was approved by the ethics committee of the University Medical Center Freiburg, Germany (Ethik‐Kommission Albert‐Ludwigs‐Universität, Freiburg) No 337/04. This study was performed in accordance with the Helsinki Declaration of 1964, as revised in 2013 and with EQUATOR (Enhancing the QUAlity and Transparency Of health Research) guidelines. Before en...
Oral implants enlarge the treatment options to replace missing teeth and have been proven to be successful as shown in systematic reviews with long‐term follow‐up. Although survival rates appear convincing, peri‐implantitis around dental implants is a challenge in daily practice, with a prevalence around 20%. The prevalence rate of peri‐implantitis is highly variable and seems to be affec...
Background
Aim of the pilot study was the histologic classification of the inflamed peri‐implant soft tissue around ceramic implants (CI) in comparison with titanium implants (TI).
Methods
Peri‐implant tissue were retrieved from 15 patients (aged 34 to 88 years, seven males/eight females) with severe peri‐implantitis (eight CI, seven TI). The peri‐implant soft tissue samples were retrie...