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Background : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

Background : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

author: Yutaka Kitamura, Masashi Suzuki, Tsuneyuki Tsukioka, Kazushige Isobe, Tetsuhiro Tsujino, Taisuke Watanabe, Takao Watanabe, Hajim | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

In this study, we focused on the possibility of spectrophotometric determination and validated the applicability of the proposed method on platelet counts in PRP preparations. This idea was based on bacterial cell counting [12] and a similar challenge was reported in 1992 [13]. However, this optical method has not been further modified for PRP as a grafting material for regenerative therapy in accordance with the policy of quality assurance. Based on the count of white blood cells (WBCs) and red blood cells (RBCs) included in PRP preparations, we categorized PRP preparations into two types as follows: pure PRP (P-PRP) and leukocyte-rich PRP (L-PRP) [14,15,16]. As not only platelets but also WBCs are concentrated in L-PRP, we hypothesized that the inclusion of WBCs at higher levels could markedly interfere with this spectrophotometric determination. As predicted, we validated the applicability of our proposed method by precisely determining platelet counts in P-PRP, but not L-PRP.

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