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Results : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

Results : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

author: Yutaka Kitamura, Masashi Suzuki, Tsuneyuki Tsukioka, Kazushige Isobe, Tetsuhiro Tsujino, Taisuke Watanabe, Takao Watanabe, Hajim | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

For validation of these calibration curves, P-PRP and L-PRP preparations prepared by three independent operators were employed. Blood cell counts are shown in Fig. 5. As observed in the calibration curves for the samples, significant differences were found in WBC and RBC counts, but not in platelet counts, between the P-PRP and L-PRP preparations. Correlations between platelet counts and WBC or RBC counts are shown in Fig. 6. Unexpectedly, strong positive correlations were observed only between platelet and RBC counts, but not between platelet and WBC counts, in both types of PRP preparations.

Measured versus calculated platelet counts are plotted in Fig. 7. In P-PRP preparations, the ratio of calculated platelet counts to the measured values was 108.6 ± 22.0%, whereas in L-PRP preparations, the ratio was 110.4 ± 64.0%. The discrepancy of SD values was reflected more clearly in the difference of R2 values (0.671 vs. 0.0605).

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