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Discussion : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

Discussion : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

author: Yutaka Kitamura, Masashi Suzuki, Tsuneyuki Tsukioka, Kazushige Isobe, Tetsuhiro Tsujino, Taisuke Watanabe, Takao Watanabe, Hajim | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

Another limitation is the color of plasma. In terms of color, blood samples obtained from the donors participating in this study were light yellow and could be evaluated as “normal.” However, we have sometimes encountered colored plasma samples in clinical practice. For example, when blood triglyceride levels are high, the plasma turns milky white or turbid [22,23,24]. Hemolytic plasma looks reddish, while icteric plasma appears yellow. When the degree of color change is not severe and when the transparency is maintained, the data may be compensated by the absorbance of PPP. However, in this case, we recommend the use of an AHA for accurate determination of the platelet counts.

We should discuss briefly how clinicians can perform quality assurance for individual PRP preparations. As described elsewhere [10, 25], PRP quality is evaluated mainly based on two major points: sterility and efficacy. Recent advances in PCR technology enable clinicians to quickly assess the contamination of targeted bacteria and mycoplasmas [26] in clinical settings. However, clinicians may require a well-trained operator for this kind of sterility testing. The current regulatory framework for PRP therapy in Japan requires clinicians to prepare PRP on a clean bench [10]. Therefore, as long as blood samples are handled aseptically, the resulting PRP preparations are evaluated as sterile.

As for efficacy, regardless of the assay system, several hours or days are required to complete efficacy testing. Even if it takes only several hours, unfortunately, this delay is not beneficial to many patients and is not suitable for on-site preparation and immediate use in autologous PRP therapy. The only exception is platelet counting, which takes only a few minutes with the use of an AHA. However, it is a problem that the conventional form of this device is $10,000 or higher and requires installation space (500 × 500 mm at least). In contrast, the compact SPM used in this study costs only $800 and can be stored in a drawer. Therefore, despite several limitations, this compact SPM would be useful for fundamental quality assurance as well as for the examination of possible correlations between platelet counts and clinical outcomes.

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