Methods : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
Using the data obtained with both the AHA and SPM, scattered plots were created to examine correlations and obtain formulas to calculate platelet counts.
P-PRP and L-PRP preparations were independently prepared from the 11 donors by three well-trained operators. Platelet counts were first determined using the AHA and aliquots of the PRP preparations were measured using the SPM. Platelet counts were calculated with the appropriate formulas and were compared with the measured platelet counts.
The data are expressed as mean ± standard deviation (SD). For two-group comparisons, statistical analyses were conducted to compare the mean values using the Student’s t test (SigmaPlot 12.5; Systat Software, Inc., San Jose, CA, USA). P values of < 0.05 were considered statistically significant. The strength of a linear association between measured platelet counts and absorbance values was evaluated using the Pearson correlation coefficient (R). Based on these data, we obtained formulas for calculating platelet counts using absorbance values. Additionally, possible correlations between platelets and RBCs or WBCs and those between measured and calculated platelet counts were also evaluated using the Pearson correlation coefficient.
Serial posts:
- Abstract : Spectrophotometric determination of platelet counts in platelet-rich plasma
- Background : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
- Background : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
- Methods : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
- Methods : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
- Results : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
- Results : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
- Discussion : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
- Discussion : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
- Discussion : Spectrophotometric determination of platelet counts in platelet-rich plasma [3]
- Conclusions : Spectrophotometric determination of platelet counts in platelet-rich plasma
- Abbreviations : Spectrophotometric determination of platelet counts in platelet-rich plasma
- References : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
- References : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]
- References : Spectrophotometric determination of platelet counts in platelet-rich plasma [3]
- References : Spectrophotometric determination of platelet counts in platelet-rich plasma [4]
- Availability of data and materials : Spectrophotometric determination of platelet counts in platelet-rich plasma
- Author information : Spectrophotometric determination of platelet counts in platelet-rich plasma [1]
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- About this article : Spectrophotometric determination of platelet counts in platelet-rich plasma
- Fig. 1. A compact SPM with its remote controller installed on an iPad Air. iPhones and other Android devices can be used instead of the iPad Air : Spectrophotometric determination of platelet count
- Fig. 2. The appearance of blood sampled after gravity fractionation and the resulting P-PRP and L-PRP. In the first low-speed spin, samples were centrifuged for 10 min at 533×g. For P-PRP preparation, the upper plasma fraction, which was 2 mm beyond the interface between plasma and RBC fractions, was transferred into sample tubes for the second high-speed spin (2656×g, 5 min). In contrast, for L-PRP preparation, the upper plasma fraction including the buffy coat and the surface of the RBC fraction was used for the second spin. The supernatant (PPP) was excluded by 50–70%, and platelets were resuspended in the remaining PPP fraction : Spectrophotometric determination of platelet count
- Fig. 3. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for calibration curves. N = 14 for each type of PRP : Spectrophotometric determination of platelet count
- Fig. 4. Calibration curves of measured platelet counts versus absorbance in P-PRP and L-PRP preparations. The samples were serially diluted by PPP, and the platelet counts were determined using an AHA and SPM. N = 14 for each type of PRP : Spectrophotometric determination of platelet count
- Fig. 5. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for validation testing. N = 32 and 50 for P-PRP and L-PRP, respectively : Spectrophotometric determination of platelet count
- Fig. 6. Scatter plots representing possible correlations between platelet (PLT) and WBC counts and between platelet and RBC counts in P-PRP and L-PRP preparations. Note: strong positive correlations were observed between platelets and RBC in both PRP types. N = 32 and 50 for P-PRP and L-PRP, respectively : Spectrophotometric determination of platelet count
- Fig. 7. Scatter plots representing correlations between measured and calculated platelet counts in P-PRP and L-PRP preparations. Note: a strong correlation was observed only in P-PRP. N = 32 and 50 for P-PRP and L-PRP, respectively : Spectrophotometric determination of platelet count