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Methods : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

Methods : Spectrophotometric determination of platelet counts in platelet-rich plasma [2]

author: Yutaka Kitamura, Masashi Suzuki, Tsuneyuki Tsukioka, Kazushige Isobe, Tetsuhiro Tsujino, Taisuke Watanabe, Takao Watanabe, Hajim | publisher: drg. Andreas Tjandra, Sp. Perio, FISID

Using the data obtained with both the AHA and SPM, scattered plots were created to examine correlations and obtain formulas to calculate platelet counts.

P-PRP and L-PRP preparations were independently prepared from the 11 donors by three well-trained operators. Platelet counts were first determined using the AHA and aliquots of the PRP preparations were measured using the SPM. Platelet counts were calculated with the appropriate formulas and were compared with the measured platelet counts.

The data are expressed as mean ± standard deviation (SD). For two-group comparisons, statistical analyses were conducted to compare the mean values using the Student’s t test (SigmaPlot 12.5; Systat Software, Inc., San Jose, CA, USA). P values of < 0.05 were considered statistically significant. The strength of a linear association between measured platelet counts and absorbance values was evaluated using the Pearson correlation coefficient (R). Based on these data, we obtained formulas for calculating platelet counts using absorbance values. Additionally, possible correlations between platelets and RBCs or WBCs and those between measured and calculated platelet counts were also evaluated using the Pearson correlation coefficient.

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