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Fig. 4. Implant placement surgery after 4 months...

Fig. 4. Implant placement surgery after 4 months of healing (a). Immediate loading of the new implants and the explanation of the implant at the left first molar (b). Panoramic radiograph showing the case finished with 12 months of follow-up (c). Clinical image showing the definitive screw-retained prostheses (d) Fig. 4. Implant placement surgery after 4 months of healing (a). Immediate...

Fig. 3. Panoramic radiograph showing excessive mar...

Fig. 3. Panoramic radiograph showing excessive marginal bone loss affecting all the dental implants in the mandible supporting fixed prostheses (a). Clinical image showing the advanced bone destruction around the implants at the incisors and left premolar regions (b). Clinical image showing the preservation of the pre-existing bone upon implant removal with the counter-torque regions (c). Panor...

Fig. 2. The cause of implant removal : Performance...

Fig. 2. The cause of implant removal Fig. 2. The cause of implant removal

Fig. 1. Frequency distribution of the location of ...

Fig. 1. Frequency distribution of the location of the explanted dental implant Fig. 1. Frequency distribution of the location of the explanted dental implant

Table 1 Description of the implants fractured duri...

Patient Location Manufacturer Abutment connection Type of fracture Location of the fracture Category of bone support around the implant (%) Use of trephine bur 1 25 Unknown Internal Complete fracture Implant body (apical third) 76–100 No 14 Unknown Internal Complete fracture Implant body (apical third) 76–100 Yes 2 15 Nobel Internal Fissure line...

About this article : Performance of the counter-to...

Anitua, E., Fernandez-de-Retana, S. & Alkhraisat, M.H. Performance of the counter-torque technique in the explantation of nonmobile dental implants. Int J Implant Dent 6, 1 (2020). https://doi.org/10.1186/s40729-019-0197-z Download citation Received: 30 July 2019 Accepted: 22 November 2019 Published: 09 January 2020 DOI: https://doi.org/10.1186/s40729-019-0197-z

Rights and permissions : Performance of the counte...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Additional information : Performance of the counte...

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Ethics declarations : Performance of the counter-t...

This study was performed following the Helsinki Declaration regarding the investigation with human subjects. Not applicable Eduardo Anitua is the Scientific Director of BTI Biotechnology Institute (Vitoria, Spain). He is the head of the Foundation Eduardo Anitua, Vitoria, Spain. Sofia Fernandez-de-Retana and Mohammad H. Alkhraisat are researchers at BTI Biotechnology Institute (Vitoria, Spain).

Author information : Performance of the counter-to...

Clínica Eduardo Anitua, Vitoria, Spain Eduardo Anitua BTI Biotechnology Institute, Vitoria, Spain Eduardo Anitua, Sofia Fernandez-de-Retana & Mohammad H. Alkhraisat Eduardo Anitua Foundation, C/Jose Maria Cagigal 19, 01007, Vitoria, Spain Eduardo Anitua You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can al...

Funding : Performance of the counter-torque techni...

No funding was received for this study.

Acknowledgements : Performance of the counter-torq...

Not applicable

References : Performance of the counter-torque tec...

Howe M-S, Keys W, Richards D. Long-term (10-year) dental implant survival: a systematic review and sensitivity meta-analysis. J Dent. 2019. https://doi.org/10.1016/j.jdent.2019.03.008. Buser D, Sennerby L, De Bruyn H. Modern implant dentistry based on osseointegration: 50 years of progress, current trends and open questions. Periodontol 2000. 2017;73(1):7–21. https://doi.org/10.1111/prd.12185. ...

Availability of data and materials : Performance o...

Data will not be shared but are available upon request.

Conclusions : Performance of the counter-torque te...

The good performance of the counter-torque method, as an atraumatic alternative for nonmobile implant removal, reinforces its use in the clinical practice. However, the technique is not exempt from complications (although at a very low rate).

Discussion : Performance of the counter-torque tec...

To our knowledge, this is the study with the largest sample size reporting the performance, in the clinical practice, of the application of counter-torque technique for the removal of nonmobile dental implants. In this retrospective analysis, the counter-torque method demonstrated to be highly predictable, showing a high success rate. The fracture of the implant (0.65% at the implant apical third)...

Results : Performance of the counter-torque techni...

Table 1 shows the relevant available information regarding the fractured dental implants. Ten dental implant fractures were observed in 7 patients. There were no differences regarding the location of the fractured implants, as 40% were located in the maxilla and 60% were located in posterior areas (Table 1). Postoperative recovery of the patients was uneventful and pain was successfully managed ...

Results : Performance of the counter-torque techni...

In this report, 749 nonmobile dental implants were explanted in 355 patients. Figure 1 shows the anatomical location of the dental implants. The 50.6% of the removed implants were located in the maxilla and 48.2% of the cases were located in the anterior areas. Attending to the causes of explantation, the vast majority of the explantations occurred due to biological complications (86.2%), followe...

Methods : Performance of the counter-torque techni...

Patients of legal age treated for implant explantation of nonmobile dental implants between March 2010 and December 2018 were included in this retrospective study. The treatment was performed using an implant removal kit (BTI Biotechnology Institute, Vitoria, Spain) that allowed the application of a counter-torque to the bone-implant interface [4]. The decision to raise a flap or not was made acc...

Background : Performance of the counter-torque tec...

Although presenting high and acceptable survival rates [1, 2], dental implants fail as a consequence of infection (periimplantitis), excessive biomechanical stress, or improper positioning. Different techniques have been described to remove a failed dental implant that include block resection, buccal bone osteotomy, trephine osteotomy, and piezosurgery [3, 4]. The application of counter-torque to ...

Abstract : Performance of the counter-torque techn...

The application of the counter-torque technique has been proposed as a conservative and atraumatic alternative for the explantation of nonmobile dental implants. The objective of this report is to assess the performance of this technique in a large number of patients. Three hundred and fifty-five patients were treated for the explantation of 749 nonmobile dental implants. The explantations were p...

Fig. 1. Approval of the Bioethics Committee of the...

Fig. 1. Approval of the Bioethics Committee of the Santa Maria University Fig. 1. Approval of the Bioethics Committee of the Santa Maria University

About this article : “Reverse torque of 30 Ncm ...

Simeone, S.G., Rios, M. & Simonpietri, J. “Reverse torque of 30 Ncm applied to dental implants as test for osseointegration”—a human observational study. Int J Implant Dent 2, 26 (2016). https://doi.org/10.1186/s40729-016-0060-4 Download citation Received: 12 September 2016 Accepted: 22 November 2016 Published: 07 December 2016 DOI: https://doi.org/10.1186/s40729-016-0060-4

Rights and permissions : “Reverse torque of 30 ...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Author information : “Reverse torque of 30 Ncm ...

Santa Maria University, Calle La Línea, Residencias El Parque, Piso 5, Apto B–5B. La Carlota. Municipio Sucre, Caracas, 1071, Venezuela Sabrina G. Simeone, María Rios & Jeannette Simonpietri You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar Corresponde...

References : “Reverse torque of 30 Ncm applied ...

Carvalho C, Carvalho L, Costa L, et al. Titanium implants: a removal torque study in osteopenic rabbits. Indian J Dent Res. 2010;21(3):349–52. Gehrke S, Dos Santos M, Dedavid B, et al. Potencial de Osseointegra¢ão versus características da superficie microtexturizada Vellox. Revista Implant News. 2011;8:229–37. Mall N, Dhanasekar B, Aparna I. Validation of implant stability: a measure of ...

References : “Reverse torque of 30 Ncm applied ...

Cehreli M, Karasoy D, Akca K, et al. Meta-analysis of methods used to assess implant stability. Int J Oral Maxillofac Implants. 2009;24(6):1015–32. Gomes J, Campos F, Marin C, et al. Implant biomechanical stability variation at early implantation times in vivo: an experimental study in dogs. J Oral Maxillofac Implants. 2013;28(3):128–34. Gehrke S. Acondicionamiento de la textura superficial ...

Conclusions : “Reverse torque of 30 Ncm applied...

Under the ideal conditions of the study herein, the application of a 30-Ncm reverse torque at the time of the stage 2 surgery, before the prosthetic restoration, allowed the clinical identification of the stability of 40 dental implants. Primary stability depends on the micro and macroscopic characteristics of the dental implant. Thus, after the application of a 30-Ncm reverse torque, both implan...

Discussion : “Reverse torque of 30 Ncm applied ...

In order to exclude risk factors, as there are no previous researches on reverse torque in humans, the research was made following a conventional protocol, only on lower jaw implants, with implants of treated surface, which have benefits that greatly compensate for the risks, allowing for faster osseointegration levels because the rugosity increases the contact surface between the implant and the ...

Discussion : “Reverse torque of 30 Ncm applied ...

The reverse torque technique proposed by Roberts in 1984 to evaluate dental implants integration proved to be successful. The most recent researches are aimed at evaluating current results. Thus, the objective of this research was to evaluate, by means of the 30 Ncm reverse torque test, 40 dental implants on the lower jaw, during the stage 2 surgery, as a mechanical and clinical, non-invasive tre...

Results : “Reverse torque of 30 Ncm applied to ...

This study reports on the clinical behavior of 40 dental implants placed on the lower jaw, on ideal bone, and under ideal surgical and systemic conditions, subjected to a 30-Ncm reverse torque during the stage 2 surgery, on 17 patients. The patients evaluated only showed normal signs and symptoms of post-operatory swelling. According to the distribution of the implants on the mandibular region, 2...

Methods : “Reverse torque of 30 Ncm applied to ...

On the data collection table, the existence of any peri-implant radiolucency radiographic images previous to the stage 2 surgery was recorded. After the application of the 30 Ncm reverse torque, the presence or absence of dental implant movement was recorded, as well as any incidence of symptomatology (pain), or presence of any clinical signs.

Methods : “Reverse torque of 30 Ncm applied to ...

A clinical trial was carried out, in which 17 patients treated with implants at the School of Dentistry of the Santa Maria University and which had had 40 dental implants placed from two different commercial brands, with NanoTec™ and Vellox® surfaces, on the lower jaw, with the approval of the Bioethics Committee of the Santa Maria University (Fig. 1). Patients 18 years old or older; Signed...

Reverse torque in dental implants : “Reverse tor...

Since the study herein is not found in humans [5, 9], last phase of study, and counting on previous results from in vitro and in vivo researches with animal models on long bones (femur, tibia, mandibles) in dogs, pigs, baboons, rabbits, and even in mastoid bones [6, 9–16], which provide necessary information to design human trials, this mechanical test was performed on dental implants in humans,...

Reverse torque in dental implants : “Reverse tor...

The reverse torque test proposed by Roberts et al. in 1984, and developed by Johansson and Albrektsson in 1987 [6], is considered as a special advantage in stage 2 surgery, because it represents a definitive clinical verification of initial integration of the dental implant with the bone surface. The torque level required is commonly expressed in Newton centimeters (Ncm) [7, 8]. This way, a clini...

Background : “Reverse torque of 30 Ncm applied ...

Primary stability has been reestablished as a previous clinical requirement to achieve osseointegration. The presence of movements between the surface of the implant and the bone tissue induces a bone resorption that produces fibrointegration, in which the implant is surrounded by an interphase of soft or connective tissue, and not bone tissue [1, 2]. Strategies used to improve bone response incl...

Abstract : “Reverse torque of 30 Ncm applied to...

On bone implantology, stability of a dental implant is an essential clinical tool during osseointegration evaluation, as it is a reflection of the structural and functional connection between the bone and the implant. The sample was comprised by 17 patients with 40 NanoTec™ and Vellox® implants, placed on the lower jaw, under optimum conditions, after a minimum healing period of 3 months, duri...

Fig. 5. Reverse torque at 30 Ncm : “Reverse tor...

Fig. 5. Reverse torque at 30 Ncm Fig. 5. Reverse torque at 30 Ncm

Fig. 4. Reverse torque test : “Reverse torque of...

Fig. 4. Reverse torque test Fig. 4. Reverse torque test

Fig. 3. 30 Ncm, measurement : “Reverse torque o...

Fig. 3. 30 Ncm, measurement Fig. 3. 30 Ncm, measurement

Fig. 2. Informed consent for patients : “Reverse...

Fig. 2. Informed consent for patients Fig. 2. Informed consent for patients

Fig. 1. Approval of the Bioethics Committee of the...

Fig. 1. Approval of the Bioethics Committee of the Santa Maria University Fig. 1. Approval of the Bioethics Committee of the Santa Maria University

About this article : “Reverse torque of 30 Ncm ...

Simeone, S.G., Rios, M. & Simonpietri, J. “Reverse torque of 30 Ncm applied to dental implants as test for osseointegration”—a human observational study. Int J Implant Dent 2, 26 (2016). https://doi.org/10.1186/s40729-016-0060-4 Download citation Received: 12 September 2016 Accepted: 22 November 2016 Published: 07 December 2016 DOI: https://doi.org/10.1186/s40729-016-0060-4

Rights and permissions : “Reverse torque of 30 ...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Author information : “Reverse torque of 30 Ncm ...

Santa Maria University, Calle La Línea, Residencias El Parque, Piso 5, Apto B–5B. La Carlota. Municipio Sucre, Caracas, 1071, Venezuela Sabrina G. Simeone, María Rios & Jeannette Simonpietri You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar Corresponde...

References : “Reverse torque of 30 Ncm applied ...

Carvalho C, Carvalho L, Costa L, et al. Titanium implants: a removal torque study in osteopenic rabbits. Indian J Dent Res. 2010;21(3):349–52. Gehrke S, Dos Santos M, Dedavid B, et al. Potencial de Osseointegra¢ão versus características da superficie microtexturizada Vellox. Revista Implant News. 2011;8:229–37. Mall N, Dhanasekar B, Aparna I. Validation of implant stability: a measure of ...

References : “Reverse torque of 30 Ncm applied ...

Cehreli M, Karasoy D, Akca K, et al. Meta-analysis of methods used to assess implant stability. Int J Oral Maxillofac Implants. 2009;24(6):1015–32. Gomes J, Campos F, Marin C, et al. Implant biomechanical stability variation at early implantation times in vivo: an experimental study in dogs. J Oral Maxillofac Implants. 2013;28(3):128–34. Gehrke S. Acondicionamiento de la textura superficial ...

Conclusions : “Reverse torque of 30 Ncm applied...

Under the ideal conditions of the study herein, the application of a 30-Ncm reverse torque at the time of the stage 2 surgery, before the prosthetic restoration, allowed the clinical identification of the stability of 40 dental implants. Primary stability depends on the micro and macroscopic characteristics of the dental implant. Thus, after the application of a 30-Ncm reverse torque, both implan...

Discussion : “Reverse torque of 30 Ncm applied ...

In order to exclude risk factors, as there are no previous researches on reverse torque in humans, the research was made following a conventional protocol, only on lower jaw implants, with implants of treated surface, which have benefits that greatly compensate for the risks, allowing for faster osseointegration levels because the rugosity increases the contact surface between the implant and the ...

Discussion : “Reverse torque of 30 Ncm applied ...

The reverse torque technique proposed by Roberts in 1984 to evaluate dental implants integration proved to be successful. The most recent researches are aimed at evaluating current results. Thus, the objective of this research was to evaluate, by means of the 30 Ncm reverse torque test, 40 dental implants on the lower jaw, during the stage 2 surgery, as a mechanical and clinical, non-invasive tre...

Results : “Reverse torque of 30 Ncm applied to ...

This study reports on the clinical behavior of 40 dental implants placed on the lower jaw, on ideal bone, and under ideal surgical and systemic conditions, subjected to a 30-Ncm reverse torque during the stage 2 surgery, on 17 patients. The patients evaluated only showed normal signs and symptoms of post-operatory swelling. According to the distribution of the implants on the mandibular region, 2...

Methods : “Reverse torque of 30 Ncm applied to ...

On the data collection table, the existence of any peri-implant radiolucency radiographic images previous to the stage 2 surgery was recorded. After the application of the 30 Ncm reverse torque, the presence or absence of dental implant movement was recorded, as well as any incidence of symptomatology (pain), or presence of any clinical signs.

Methods : “Reverse torque of 30 Ncm applied to ...

A clinical trial was carried out, in which 17 patients treated with implants at the School of Dentistry of the Santa Maria University and which had had 40 dental implants placed from two different commercial brands, with NanoTec™ and Vellox® surfaces, on the lower jaw, with the approval of the Bioethics Committee of the Santa Maria University (Fig. 1). Patients 18 years old or older; Signed...

Reverse torque in dental implants : “Reverse tor...

Since the study herein is not found in humans [5, 9], last phase of study, and counting on previous results from in vitro and in vivo researches with animal models on long bones (femur, tibia, mandibles) in dogs, pigs, baboons, rabbits, and even in mastoid bones [6, 9–16], which provide necessary information to design human trials, this mechanical test was performed on dental implants in humans,...

Reverse torque in dental implants : “Reverse tor...

The reverse torque test proposed by Roberts et al. in 1984, and developed by Johansson and Albrektsson in 1987 [6], is considered as a special advantage in stage 2 surgery, because it represents a definitive clinical verification of initial integration of the dental implant with the bone surface. The torque level required is commonly expressed in Newton centimeters (Ncm) [7, 8]. This way, a clini...

Background : “Reverse torque of 30 Ncm applied ...

Primary stability has been reestablished as a previous clinical requirement to achieve osseointegration. The presence of movements between the surface of the implant and the bone tissue induces a bone resorption that produces fibrointegration, in which the implant is surrounded by an interphase of soft or connective tissue, and not bone tissue [1, 2]. Strategies used to improve bone response incl...

Abstract : “Reverse torque of 30 Ncm applied to...

On bone implantology, stability of a dental implant is an essential clinical tool during osseointegration evaluation, as it is a reflection of the structural and functional connection between the bone and the implant. The sample was comprised by 17 patients with 40 NanoTec™ and Vellox® implants, placed on the lower jaw, under optimum conditions, after a minimum healing period of 3 months, duri...

About this article : “Reverse torque of 30 Ncm ...

Simeone, S.G., Rios, M. & Simonpietri, J. “Reverse torque of 30 Ncm applied to dental implants as test for osseointegration”—a human observational study. Int J Implant Dent 2, 26 (2016). https://doi.org/10.1186/s40729-016-0060-4 Download citation Received: 12 September 2016 Accepted: 22 November 2016 Published: 07 December 2016 DOI: https://doi.org/10.1186/s40729-0...

Rights and permissions : “Reverse torque of 30 ...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...

Author information : “Reverse torque of 30 Ncm ...

Santa Maria University, Calle La Línea, Residencias El Parque, Piso 5, Apto B–5B. La Carlota. Municipio Sucre, Caracas, 1071, Venezuela Sabrina G. Simeone, María Rios & Jeannette Simonpietri You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for ...

References : “Reverse torque of 30 Ncm applied ...

Carvalho C, Carvalho L, Costa L, et al. Titanium implants: a removal torque study in osteopenic rabbits. Indian J Dent Res. 2010;21(3):349–52. Gehrke S, Dos Santos M, Dedavid B, et al. Potencial de Osseointegra¢ão versus características da superficie microtexturizada Vellox. Revista Implant News. 2011;8:229–37. Mall N, Dhanasekar B, Aparna I. Validation of implant stability: a measure of ...

References : “Reverse torque of 30 Ncm applied ...

Cehreli M, Karasoy D, Akca K, et al. Meta-analysis of methods used to assess implant stability. Int J Oral Maxillofac Implants. 2009;24(6):1015–32. Gomes J, Campos F, Marin C, et al. Implant biomechanical stability variation at early implantation times in vivo: an experimental study in dogs. J Oral Maxillofac Implants. 2013;28(3):128–34. Gehrke S. Acondicionamiento de la textura superficial ...

Conclusions : “Reverse torque of 30 Ncm applied...

Under the ideal conditions of the study herein, the application of a 30-Ncm reverse torque at the time of the stage 2 surgery, before the prosthetic restoration, allowed the clinical identification of the stability of 40 dental implants. Primary stability depends on the micro and macroscopic characteristics of the dental implant. Thus, after the application of a 30-Ncm reverse torque, both implan...

Discussion : “Reverse torque of 30 Ncm applied ...

In order to exclude risk factors, as there are no previous researches on reverse torque in humans, the research was made following a conventional protocol, only on lower jaw implants, with implants of treated surface, which have benefits that greatly compensate for the risks, allowing for faster osseointegration levels because the rugosity increases the contact surface between the implant and the ...

Discussion : “Reverse torque of 30 Ncm applied ...

The reverse torque technique proposed by Roberts in 1984 to evaluate dental implants integration proved to be successful. The most recent researches are aimed at evaluating current results. Thus, the objective of this research was to evaluate, by means of the 30 Ncm reverse torque test, 40 dental implants on the lower jaw, during the stage 2 surgery, as a mechanical and clinical, non-invasive tre...

Results : “Reverse torque of 30 Ncm applied to ...

This study reports on the clinical behavior of 40 dental implants placed on the lower jaw, on ideal bone, and under ideal surgical and systemic conditions, subjected to a 30-Ncm reverse torque during the stage 2 surgery, on 17 patients. The patients evaluated only showed normal signs and symptoms of post-operatory swelling. According to the distribution of the implants on the mandibular region, 2...

Methods : “Reverse torque of 30 Ncm applied to ...

On the data collection table, the existence of any peri-implant radiolucency radiographic images previous to the stage 2 surgery was recorded. After the application of the 30 Ncm reverse torque, the presence or absence of dental implant movement was recorded, as well as any incidence of symptomatology (pain), or presence of any clinical signs.

Methods : “Reverse torque of 30 Ncm applied to ...

A clinical trial was carried out, in which 17 patients treated with implants at the School of Dentistry of the Santa Maria University and which had had 40 dental implants placed from two different commercial brands, with NanoTec™ and Vellox® surfaces, on the lower jaw, with the approval of the Bioethics Committee of the Santa Maria University (Fig. 1). Patients 18 years old or older; Signed...

Reverse torque in dental implants : “Reverse tor...

Since the study herein is not found in humans [5, 9], last phase of study, and counting on previous results from in vitro and in vivo researches with animal models on long bones (femur, tibia, mandibles) in dogs, pigs, baboons, rabbits, and even in mastoid bones [6, 9–16], which provide necessary information to design human trials, this mechanical test was performed on dental implants in humans,...

Reverse torque in dental implants : “Reverse tor...

The reverse torque test proposed by Roberts et al. in 1984, and developed by Johansson and Albrektsson in 1987 [6], is considered as a special advantage in stage 2 surgery, because it represents a definitive clinical verification of initial integration of the dental implant with the bone surface. The torque level required is commonly expressed in Newton centimeters (Ncm) [7, 8]. This way, a clini...

Background : “Reverse torque of 30 Ncm applied ...

Primary stability has been reestablished as a previous clinical requirement to achieve osseointegration. The presence of movements between the surface of the implant and the bone tissue induces a bone resorption that produces fibrointegration, in which the implant is surrounded by an interphase of soft or connective tissue, and not bone tissue [1, 2]. Strategies used to improve bone response incl...

Abstract : “Reverse torque of 30 Ncm applied to...

On bone implantology, stability of a dental implant is an essential clinical tool during osseointegration evaluation, as it is a reflection of the structural and functional connection between the bone and the implant. The sample was comprised by 17 patients with 40 NanoTec™ and Vellox® implants, placed on the lower jaw, under optimum conditions, after a minimum healing period of 3 months, duri...

Fig. 7. Scatter plots representing correlations be...

Fig. 7. Scatter plots representing correlations between measured and calculated platelet counts in P-PRP and L-PRP preparations. Note: a strong correlation was observed only in P-PRP. N = 32 and 50 for P-PRP and L-PRP, respectively Fig. 7. Scatter plots representing correlations between measured and calculated platelet counts in P-PRP and L-PRP preparations. Note: a strong correlation was ...

Fig. 6. Scatter plots representing possible correl...

Fig. 6. Scatter plots representing possible correlations between platelet (PLT) and WBC counts and between platelet and RBC counts in P-PRP and L-PRP preparations. Note: strong positive correlations were observed between platelets and RBC in both PRP types. N = 32 and 50 for P-PRP and L-PRP, respectively Fig. 6. Scatter plots representing possible correlations between platelet (PLT) and WB...

Fig. 5. Counts of platelets (PLT), WBCs, and RBCs ...

Fig. 5. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for validation testing. N = 32 and 50 for P-PRP and L-PRP, respectively Fig. 5. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for validation testing. N = 32 and 50 for P-PRP and L-PRP, respectively

Fig. 4. Calibration curves of measured platelet co...

Fig. 4. Calibration curves of measured platelet counts versus absorbance in P-PRP and L-PRP preparations. The samples were serially diluted by PPP, and the platelet counts were determined using an AHA and SPM. N = 14 for each type of PRP Fig. 4. Calibration curves of measured platelet counts versus absorbance in P-PRP and L-PRP preparations. The samples were serially diluted by PPP, and th...

Fig. 3. Counts of platelets (PLT), WBCs, and RBCs ...

Fig. 3. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for calibration curves. N = 14 for each type of PRP Fig. 3. Counts of platelets (PLT), WBCs, and RBCs in P-PRP and L-PRP preparations prepared for calibration curves. N = 14 for each type of PRP

Fig. 2. The appearance of blood sampled after grav...

Fig. 2. The appearance of blood sampled after gravity fractionation and the resulting P-PRP and L-PRP. In the first low-speed spin, samples were centrifuged for 10 min at 533×g. For P-PRP preparation, the upper plasma fraction, which was 2 mm beyond the interface between plasma and RBC fractions, was transferred into sample tubes for the second high-speed spin (2656×g, 5 min). In contrast, ...

Fig. 1. A compact SPM with its remote controller i...

Fig. 1. A compact SPM with its remote controller installed on an iPad Air. iPhones and other Android devices can be used instead of the iPad Air Fig. 1. A compact SPM with its remote controller installed on an iPad Air. iPhones and other Android devices can be used instead of the iPad Air

About this article : Spectrophotometric determinat...

Kitamura, Y., Suzuki, M., Tsukioka, T. et al. Spectrophotometric determination of platelet counts in platelet-rich plasma. Int J Implant Dent 4, 29 (2018). https://doi.org/10.1186/s40729-018-0140-8 Download citation Received: 13 April 2018 Accepted: 29 June 2018 Published: 02 October 2018 DOI: https://doi.org/10.1186/s40729-018-0140-8

Rights and permissions : Spectrophotometric determ...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Ethics declarations : Spectrophotometric determina...

The study design and consent forms of all the procedures performed were approved by the ethics committee for human participants of the Niigata University School of Medicine (Niigata, Japan) in accordance with the Helsinki Declaration of 1964 as revised in 2013. Written informed consents to participate in the study were obtained from all the participants. Written informed consents for the publicat...

Author information : Spectrophotometric determinat...

You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar YK, MS, TyTo, and TK conceived and designed the study, performed the experiments and data analysis, and wrote the manuscript. KI, TaTn, ...

Author information : Spectrophotometric determinat...

Tokyo Plastic Dental Society, Kita-ku, Tokyo, Japan Yutaka Kitamura, Masashi Suzuki, Tsuneyuki Tsukioka, Kazushige Isobe, Tetsuhiro Tsujino, Taisuke Watanabe, Takao Watanabe & Hajime Okudera Bioscience Medical Research Center, Niigata University Medical and Dental Hospital, Niigata, Japan Koh Nakata Department of Materials Science and Technology, Niigata University, Niigata, Japan Tak...

Availability of data and materials : Spectrophotom...

Because an article performed and prepared in parallel is now submitted elsewhere for publication, the authors do not wish to share their data at present time.

References : Spectrophotometric determination of p...

Xu Z, Yin W, Zhang Y, Qi X, Chen Y, Xie X, Zhang C. Comparative evaluation of leukocyte- and platelet-rich plasma and pure platelet-rich plasma for cartilage regeneration. Sci Rep. 2017;7:43301. Yin W, Qi X, Zhang Y, Sheng J, Xu Z, Tao S, Xie X, Li X, Zhang C. Advantages of pure platelet-rich plasma compared with leukocyte- and platelet-rich plasma in promoting repair of bone defects. J Transl Me...

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References : Spectrophotometric determination of p...

Hazan R, Que YA, Maura D, Rahme LG. A method for high throughput determination of viable bacteria cell counts in 96-well plates. BMC Microbiol. 2012;12:259. Lee VS, Tarassenko L. An optical method for the determination of platelet count in platelet samples contaminated with red blood cells. J Biochem Biophys Methods. 1992;24:215–23. Davis VL, Abukabda AB, Radio NM, Witt-Enderby PA, Clafshenkel...

References : Spectrophotometric determination of p...

Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85:638–46. Etulain J. Platelets in wound healing and regenerative medicine. Platelets. 2018:1–13. Hou X, Yuan J, Aisaiti A, Liu Y, Zhao J. The effect of platelet-rich plasma on clinical outcomes of t...

Abbreviations : Spectrophotometric determination o...

Acid-citrate-dextrose solution Automated hematology analyzer Leukocyte-rich PRP Prostaglandin E1 Platelet-poor plasma Platelet-rich fibrin Platelet-rich plasma Pure-PRP Red blood cell Standard deviation Spectrophotometer Leukocyte

Conclusions : Spectrophotometric determination of ...

In normal blood samples composed of light yellow plasma, spectrophotometric determination of platelet counts would be useful for quality assurance of individual PRP preparations. For accurate determination, however, operators should handle samples with care to minimize the inclusion of WBCs and RBCs in PRP preparations.

Discussion : Spectrophotometric determination of p...

Consistent with the clinical significance of platelet counting, several studies have reported that the platelet concentration is the most reliable criterion for the regenerative ability of PRP [27, 28] because platelets increase the number of anabolic signaling molecules. Conversely, as WBCs increase the number of catabolic signaling molecules, the quality of PRP can, perhaps, be considerably alte...

Discussion : Spectrophotometric determination of p...

Another limitation is the color of plasma. In terms of color, blood samples obtained from the donors participating in this study were light yellow and could be evaluated as “normal.” However, we have sometimes encountered colored plasma samples in clinical practice. For example, when blood triglyceride levels are high, the plasma turns milky white or turbid [22,23,24]. Hemolytic plasma looks r...

Discussion : Spectrophotometric determination of p...

Since determination of bacterial cell number is a fundamental procedure in the field of microbiology, several methods have been developed and widely employed depending on the purpose of cell counting. SPM is one of the common methods used to estimate bacterial load [12]. The advantage of SPM is speed and convenience without additional preparation steps. On the other hand, the limitations are the i...

Results : Spectrophotometric determination of plat...

For validation of these calibration curves, P-PRP and L-PRP preparations prepared by three independent operators were employed. Blood cell counts are shown in Fig. 5. As observed in the calibration curves for the samples, significant differences were found in WBC and RBC counts, but not in platelet counts, between the P-PRP and L-PRP preparations. Correlations between platelet counts and WBC or R...

Results : Spectrophotometric determination of plat...

The appearance of the blood-collection tube after the first low-speed spin and representative P-PRP and L-PRP preparations after the second high-speed spin and subsequent re-suspension are shown in Fig. 2. Although low-speed spinning did not result in the formation of a clear buffy coat in the interface between the plasma and RBC fractions, the buffy coat corresponding to the plasma was not inclu...

Methods : Spectrophotometric determination of plat...

Using the data obtained with both the AHA and SPM, scattered plots were created to examine correlations and obtain formulas to calculate platelet counts. P-PRP and L-PRP preparations were independently prepared from the 11 donors by three well-trained operators. Platelet counts were first determined using the AHA and aliquots of the PRP preparations were measured using the SPM. Platelet counts we...

Methods : Spectrophotometric determination of plat...

Blood samples were collected from 11 non-smoking healthy male volunteers aged 33 to 69 years. The study design and consent forms for all the procedures were approved by the ethics committee for human participants at the Niigata University School of Medicine (Niigata, Japan) in accordance with the Helsinki Declaration of 1964 as revised in 2013. Peripheral blood (~ 9 mL) was collected into pla...

Background : Spectrophotometric determination of p...

In this study, we focused on the possibility of spectrophotometric determination and validated the applicability of the proposed method on platelet counts in PRP preparations. This idea was based on bacterial cell counting [12] and a similar challenge was reported in 1992 [13]. However, this optical method has not been further modified for PRP as a grafting material for regenerative therapy in acc...

Background : Spectrophotometric determination of p...

Almost two decades have passed since platelet concentrates, such as platelet-rich plasma (PRP), were first introduced to the field of regenerative medicine by Marx et al. [1]. To date, PRP has been modified to create different variations and has increasingly been used in various fields of regenerative therapy around the world. However, negative data obtained from clinical applications of PRP have ...

Abstract : Spectrophotometric determination of pla...

Platelet-rich plasma (PRP) is widely used in regenerative dentistry and other medical fields. However, its effectiveness has often been questioned. For better evaluation, the quality of individual PRP preparations should be assured prior to use. We proposed a spectrophotometric method for determination of platelet counts and validated its applicability using two types of PRP preparations. Blood s...