Baseline of osseointegration and induction of periodontitis

Seven weeks after the implantation

-Group (1): 2 rats will be sacrificed and osteointegration and inflammatory markers will be examined.

-Group (2): 2 rats will receive suture ligature at the cervical part of each implant.

-Group (3): 2 rats will receive no ligature as a control.

All the procedures will be performed when rats are under anesthesia.

Identify induced peri-implantitis

Nine weeks after the implantation or 2 weeks after ligature procedure

-Group 2 and group 3 will be sacrificed and osteointegration and inflammatory markers will be examined.

Examination of implantation, osteointegration, and inflammatory markers:

X-ray: periapical X-ray to examine the implantation of implants.

Microcomputed tomography (Micro-CT)

-The rat’s skull will be scanned with Micro-CT after sacrificed. (The dissected tissues will be taken to Rutgers Piscataway campus for Micro-CT examination as per IACUC policies.)

Implant success and failure rate is analyzed by using Clopper-Pearson’s exact method at 95% confidence interval.