Fig. 3. Quantification of type I collagen via ELISA. Data representative of the experiment run in triplicate. Values are expressed as mean (± SD). The asterisk symbol indicates a significant difference between groups (p < 0.05)
Fig. 2. a Cell proliferation in gingival fibroblasts at 24 h, 48 h, and 72 h. The line chart represents the means and standard deviations from three separate experiments. b Cell viability assay in gingival fibroblast at 24 h, 48 h, and 72 h. The line chart represents the means and standard deviations from three separate experiments. The values are expressed in means (± SD). The as...
Fig. 1. Scanning electron microscopy and laser interferometry. a, d Machined surface. b, e 20-min acid treatment. c, f 60-min acid treatment. Original magnification, × 15,000
de Souza, V.Z., Manfro, R., Joly, J.C. et al. Viability and collagen secretion by fibroblasts on titanium surfaces with different acid-etching protocols.
Int J Implant Dent 5, 41 (2019). https://doi.org/10.1186/s40729-019-0192-4
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Received: 09 August 2019
Accepted: 24 October 2019
Published: 21 November 2019
DOI: https://doi.org/10.1186/s40729-019-0192-4
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This study was approved by the Research Ethics Committee of the São Leopoldo Mandic Research Institute, Campinas/SP (protocol No. 59866216.6.0000.5374).
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Vilton Zimmermann de Souza, Rafael Manfro, Júlio César Joly, Carlos Nelson Elias, Daiane Cristina Peruzzo, Marcelo Henrique Napimoga, and Elizabeth Ferreira Martinez state that they have no conflicts of interest.
VZS and EFM interpreted and analyzed the data collected, contributed to the drafting of the paper and revised it critically, and were major contributors in writing the manuscript. RM, JCJ, and CNE contributed to the concept/design of the study and the final manuscript. DCP and MHN critically revised and contributed to the final manuscript. All authors read and approved the final version to be publ...
Division of Implantology, Faculdade São Leopoldo Mandic, Campinas, SP, Brazil
Vilton Zimmermann de Souza, Júlio César Joly & Daiane Cristina Peruzzo
Division of Implantology, SOEBRÁS, Passo Fundo, RS, Brazil
Rafael Manfro
Materials Science Department, Instituto Militar de Engenharia, Rio de Janeiro, RJ, Brazil
Carlos Nelson Elias
Division of Immunology, Faculdade São Leopoldo Mandic,...
The funding received for this study was from the researcher himself.
The authors wish to thank Mrs. Pollyanna Tombini Montaldi for her excellent technical expertise and assistance.
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Schierano G, Ramieri G, Cor...
All data generated or analyzed during this study are included in this published article.
Therefore, the results of the present study indicate that the acid treatments used did not compromise cell growth nor collagen synthesis by gingival fibroblasts compared to machined titanium. The cost-benefit of manufacturing prosthetic abutments with treated surfaces should however be considered. In addition, in vitro studies using human keratinocytes and histological studies in vivo should be pe...
The connective tissue seal around the abutment is crucial for perimplant health. Several efforts have previously been made to optimize abutment surfaces, though without consensus on the ideal surface features. Blasquez et al. [11] have shown in their systematic review that different types of surface modifications for implant abutments may provide benefit to the surrounding connective tissue, which...
The results showed that, regardless of the treatment used, there was no difference in the values of cell proliferation in the different times of analysis. Similarly, cell viability values did not differ, especially after 24 h and 72 h. Corroborating the results of this study, Baltriukiene et al. [17] used grade 2 titanium disks with different surface treatments and demonstrated that the modifi...
Implant dentistry has for years focused on studying the interface between the bone and implant, searching for a suitable seal to prevent the advance of perimplant disease. Long-term prognosis of dental implants, however, depends not only on osseointegration, but also on the quality of the seal between the mucosa and the implant abutment [13].
The seal provided by the contact between mucosal tissu...
The results showed that acid treatment of the titanium surfaces for 20 or 60 min did not affect cell proliferation, either after 24 h (p = 0.484), 48 h (p = 0.698), or 72 h (p = 0.287) (Fig. 2a).
For the cell viability test, loss of viability was observed for the machined surface after 48 h of culture compared to the groups submitted to acid treatment for 20 or 60 min, which did not...
The experiments were repeated three times, under the same conditions, to ensure accuracy.
Quantification of secreted type I collagen by fibroblast cultures on to the different surfaces was evaluated by enzyme-linked immunosorbent assay (ELISA). The supernatant was collected and centrifuged at 336g for 10 min. Type I collagen quantification was carried out using the Human Type I Collagen kit (R&...
This study was approved by the Research Ethics Committee of the São Leopoldo Mandic Research Institute, Campinas/SP (protocol No. 59866216.6.0000.5374). Titanium disks were commercially pure, grade 4 (n = 108), measuring 6 mm in diameter by 2 mm in thickness, provided by the company Conexão Sistemas de Próteses (Arujá, São Paulo).
For the treatment of titanium disk surfaces, sulfuric, ni...
Consolidation of surface treatments for dental implants and knowledge on the cellular mechanisms of osseointegration has propelled research on the sealing capacity of bone to implant surfaces. Although osseointegration is extremely important for implant success, biological sealing of the perimplantar connective tissue is crucial to maintain success in the long-term because it acts as a first barri...
Pengukuran tulang (bone sounding)
Bone sounding atau transmucosal sounding (TS) adalah pengukuran yang digunakan untuk menentukan ketinggian seluruh cuff (manset) jaringan lunak pada berbagai kelompok gigi dan implant. Matra mukosa peri-implan dan gingiva pada letak gigi yang berdekatan dipelajari dengan pengukuran klinis yang dilakukan terutama pada subjek edentulous sebagian yang telah dirawa...
The implant device lacks tooth characteristic structures such as root cementum, periodontal ligament, and bundle bone (alveolar bone proper). The dento‐alveolar and the dento‐gingival fiber bundles connect the soft tissues with the tooth (root cementum), while no such fiber bundles are apparent in the peri‐implant tissues. At periodontally healthy sites, the margin of the gingiva follow...
Bone tissue in the edentulous ridge
In a study involving partially edentulous subjects, hard tissue biopsies were sampled from the maxilla and the mandible with the use of trephine drills. The bone tissue was found to include a blend of mainly lamellar bone (46%) and bone marrow (23%) with less amounts of fibrous (12%) and osteoid (4%) tissue. Bone marrow was the dominant tissu...
KERATINIZED MUCOSA (KM)
Keratinized mucosa is a term used to describe the masticatory mucosa that is present at many, but not all, implant sites. Keratinized mucosa extends from the margin of the peri‐implant mucosa to the movable lining (oral) mucosa. Keratinized mucosa is comprised of a lamina propria (fibrous connective tissue that contains fibroblasts and equal amounts of ty...
BONE SOUNDING
Bone sounding or transmucosal sounding (TS) is a measurement that is used to determine the height of the entire soft tissue cuff at various groups of teeth and implants. The dimensions of the peri‐implant mucosa and the gingiva at adjacent tooth sites was studied by clinical measurements performed mainly in partially edentulous subjects who had been treated with im...
PERI‐IMPLANT TISSUES IN CLINICAL HEALTH
The gingiva and the peri‐implant mucosa and their adhesion (seal) are consistently challenged by the oral environment, including the steady exposure to microorganisms in the biofilm present on the tooth and implant surfaces. In the clinically normal peri‐implant mucosa (and gingiva), the continuous host response includes both vascular ...
Animal studies
The dimension of the peri‐implant mucosa, often called the biological width or dimension, was examined in biopsies mainly obtained from studies in dogs. Such measurements disclosed that a certain width of soft tissue may be required to cover the peri‐implant bone. The studies referred to the length of the epithelium (from the peri‐implant mucosa margin to the...
The formation of the mucosal adhesion was studied in a dog model. One‐piece implant devices were placed in the edentulous mandible of dogs, and healing was monitored using light microscopic examination of biopsies sampled at different intervals during a 3‐month period. In the initial phase of the wound between the implant and cut connective tissue, a fibrin clot/coagulum formed that was inf...
PERI‐IMPLANT MUCOSA
Most information regarding the structural features of the peri‐implant mucosa is derived from animal studies using dog models. In such studies implants were placed in the edentulous ridge (alternatively, the fresh extraction socket), the outer osseous part of which was covered with masticatory mucosa. It was also shown that the healed peri‐implant mucosa on the buccal a...
Peri‐implant tissues are those that occur around osseointegrated dental implants. They are divided into soft and hard tissue compartments. The soft tissue compartment is denoted “peri‐implant mucosa” and is formed during the wound healing process that follows implant/abutment placement. The hard tissue compartment forms a contact relationship to the implant surface to secure implant stab...
Abstract
Objective
The aim is to define clinical and histologic characteristics of peri‐implant tissues in health and describe the mucosa–implant interface.
Importance
An understanding of the characteristics of healthy peri‐implant tissues facilitates the recognition of disease (i.e., departure from health).
Findings
The healthy peri‐implant mucosa is, at the microscopic ...
Figure 48. Dimensional ridge alterations: 4 weeks
At 4 weeks after tooth extraction, the socket is filled with woven bone. Osteoclasts are present on the outer surfaces at the margin of the buccal and lingual walls, signaling resorption of cortical plates. The resorption of the bundle bone is almost complete. Osteoclasts also line the trabeculae of woven bone present in the central and latera...
Figure 47. Dimensional ridge alterations: 2 weeks
At 2 weeks after tooth extraction, the apical and lateral parts of the socket are filled with woven bone, while the central and marginal portions of the socket are occupied by provisional connective tissue. On the inner and outer surfaces of the socket walls, numerous osteoclasts can be seen. In several areas of the socket wall, the bundle bon...
Figure 34. Day 4 - 14 : Epithelial proliferation & connective tissue
After 4 to 5 days, the epithelium from the margins of the soft tissue starts to proliferate to cover the granulation tissue in the socket. At 14 days, the connective tissue at the marginal portion of the extraction socket is partially lined by epithelial cells.
Figure 30. Quantitative tissue analysis
Trombelli and coworkers monitored the healing of human extraction sockets for a 6-month period and presented a semi-quantitative analysis of tissues and cell populations involved in various stages of socket healing. They showed that granulation tissue was present in comparatively large amounts in the early phases of socket healing. At 6 to 8 weeks, th...
Figure 26. Day 14: woven bone & connective tissue
After 14 days of healing woven bone, which appears as a loose, unstructured network of bone, has started to fill the socket except in the central region, where significant amounts of the provisional connective tissue matrix still remain. This is because the woven bone forms first at the periphery of the socket and gradually extends from the wa...