Open hour: senin - sabtu 09:00:00 - 20:00:00; minggu & tanggal merah tutup

Fig. 3. Quantification of type I collagen via ELIS...

Fig. 3. Quantification of type I collagen via ELISA. Data representative of the experiment run in triplicate. Values are expressed as mean (± SD). The asterisk symbol indicates a significant difference between groups (p < 0.05)

Fig. 2. a Cell proliferation in gingival fibroblas...

Fig. 2. a Cell proliferation in gingival fibroblasts at 24 h, 48 h, and 72 h. The line chart represents the means and standard deviations from three separate experiments. b Cell viability assay in gingival fibroblast at 24 h, 48 h, and 72 h. The line chart represents the means and standard deviations from three separate experiments. The values are expressed in means (± SD). The as...

Fig. 1. Scanning electron microscopy and laser int...

Fig. 1. Scanning electron microscopy and laser interferometry. a, d Machined surface. b, e 20-min acid treatment. c, f 60-min acid treatment. Original magnification, × 15,000

About this article : Viability and collagen secret...

de Souza, V.Z., Manfro, R., Joly, J.C. et al. Viability and collagen secretion by fibroblasts on titanium surfaces with different acid-etching protocols. Int J Implant Dent 5, 41 (2019). https://doi.org/10.1186/s40729-019-0192-4 Download citation Received: 09 August 2019 Accepted: 24 October 2019 Published: 21 November 2019 DOI: https://doi.org/10.1186/s40729-019-0192-4

Rights and permissions : Viability and collagen se...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Additional information : Viability and collagen se...

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Ethics declarations : Viability and collagen secre...

This study was approved by the Research Ethics Committee of the São Leopoldo Mandic Research Institute, Campinas/SP (protocol No. 59866216.6.0000.5374). Not applicable. Vilton Zimmermann de Souza, Rafael Manfro, Júlio César Joly, Carlos Nelson Elias, Daiane Cristina Peruzzo, Marcelo Henrique Napimoga, and Elizabeth Ferreira Martinez state that they have no conflicts of interest.

Author information : Viability and collagen secret...

VZS and EFM interpreted and analyzed the data collected, contributed to the drafting of the paper and revised it critically, and were major contributors in writing the manuscript. RM, JCJ, and CNE contributed to the concept/design of the study and the final manuscript. DCP and MHN critically revised and contributed to the final manuscript. All authors read and approved the final version to be publ...

Author information : Viability and collagen secret...

Division of Implantology, Faculdade São Leopoldo Mandic, Campinas, SP, Brazil Vilton Zimmermann de Souza, Júlio César Joly & Daiane Cristina Peruzzo Division of Implantology, SOEBRÁS, Passo Fundo, RS, Brazil Rafael Manfro Materials Science Department, Instituto Militar de Engenharia, Rio de Janeiro, RJ, Brazil Carlos Nelson Elias Division of Immunology, Faculdade São Leopoldo Mandic,...

Funding : Viability and collagen secretion by fibr...

The funding received for this study was from the researcher himself.

Acknowledgements : Viability and collagen secretio...

The authors wish to thank Mrs. Pollyanna Tombini Montaldi for her excellent technical expertise and assistance.

References : Viability and collagen secretion by f...

Velasco-Ortega E, Alfonso-Rodríguez CA, Monsalve-Guil L, España-López A, Jiménez-Guerra A, Garzón I, Alaminos M, Gil FJ. Relevant aspects in the surface properties in titanium dental implants for the cellular viability. Mater Sci Eng C Mater Biol Appl. 2016;64:1–10. Cao J, Wang T, Pu Y, Tang Z, Meng H. Influence on proliferation and adhesion of human gingival fibroblasts from different tit...

References : Viability and collagen secretion by f...

Martinez EF, Araújo VC. In vitro immunoexpression of extracellular matrix proteins in dental pulpal and gingival human fibroblasts. Int Endod J. 2004;37(11):749–55. Lee HJ, Lee J, Lee JT, Hong JS, Lim BS, Park HJ, Kim YK, Kim TI. Microgrooves on titanium surface affect peri-implant cell adhesion and soft tissue sealing; an in vitro and in vivo study. J Periodontal Implant Sci. ...

References : Viability and collagen secretion by f...

Chai WL, Moharamzadeh K, Brook IM, Van Noort R. A review of histomorphometric analysis techniques for assessing implant-soft tissue interface. Biotech Histochem. 2011;86(4):242–54. Buser D, Weber HP, Donath K, Fiorellini JP, Paquette DW, Williams RC. Soft tissue reactions to non-submerged unloaded titanium implants in beagle dogs. J Periodontol. 1992;63(3):225–35. Schierano G, Ramieri G, Cor...

Availability of data and materials : Viability and...

All data generated or analyzed during this study are included in this published article.

Conclusions : Viability and collagen secretion by ...

Therefore, the results of the present study indicate that the acid treatments used did not compromise cell growth nor collagen synthesis by gingival fibroblasts compared to machined titanium. The cost-benefit of manufacturing prosthetic abutments with treated surfaces should however be considered. In addition, in vitro studies using human keratinocytes and histological studies in vivo should be pe...

Discussion : Viability and collagen secretion by f...

The connective tissue seal around the abutment is crucial for perimplant health. Several efforts have previously been made to optimize abutment surfaces, though without consensus on the ideal surface features. Blasquez et al. [11] have shown in their systematic review that different types of surface modifications for implant abutments may provide benefit to the surrounding connective tissue, which...

Discussion : Viability and collagen secretion by f...

The results showed that, regardless of the treatment used, there was no difference in the values of cell proliferation in the different times of analysis. Similarly, cell viability values did not differ, especially after 24 h and 72 h. Corroborating the results of this study, Baltriukiene et al. [17] used grade 2 titanium disks with different surface treatments and demonstrated that the modifi...

Discussion : Viability and collagen secretion by f...

Implant dentistry has for years focused on studying the interface between the bone and implant, searching for a suitable seal to prevent the advance of perimplant disease. Long-term prognosis of dental implants, however, depends not only on osseointegration, but also on the quality of the seal between the mucosa and the implant abutment [13]. The seal provided by the contact between mucosal tissu...

Results : Viability and collagen secretion by fibr...

The results showed that acid treatment of the titanium surfaces for 20 or 60 min did not affect cell proliferation, either after 24 h (p = 0.484), 48 h (p = 0.698), or 72 h (p = 0.287) (Fig. 2a). For the cell viability test, loss of viability was observed for the machined surface after 48 h of culture compared to the groups submitted to acid treatment for 20 or 60 min, which did not...

Material and methods : Viability and collagen secr...

The experiments were repeated three times, under the same conditions, to ensure accuracy. Quantification of secreted type I collagen by fibroblast cultures on to the different surfaces was evaluated by enzyme-linked immunosorbent assay (ELISA). The supernatant was collected and centrifuged at 336g for 10 min. Type I collagen quantification was carried out using the Human Type I Collagen kit (R&...

Material and methods : Viability and collagen secr...

This study was approved by the Research Ethics Committee of the São Leopoldo Mandic Research Institute, Campinas/SP (protocol No. 59866216.6.0000.5374). Titanium disks were commercially pure, grade 4 (n = 108), measuring 6 mm in diameter by 2 mm in thickness, provided by the company Conexão Sistemas de Próteses (Arujá, São Paulo). For the treatment of titanium disk surfaces, sulfuric, ni...

Introduction : Viability and collagen secretion by...

Consolidation of surface treatments for dental implants and knowledge on the cellular mechanisms of osseointegration has propelled research on the sealing capacity of bone to implant surfaces. Although osseointegration is extremely important for implant success, biological sealing of the perimplantar connective tissue is crucial to maintain success in the long-term because it acts as a first barri...

Pengukuran tulang : kesarasan peri-implant

Pengukuran tulang (bone sounding) Bone sounding atau transmucosal sounding (TS) adalah pengukuran yang digunakan untuk menentukan ketinggian seluruh cuff (manset) jaringan lunak  pada berbagai kelompok gigi dan implant. Matra mukosa peri-implan dan gingiva pada letak gigi yang berdekatan dipelajari dengan pengukuran klinis yang dilakukan terutama pada subjek edentulous sebagian yang telah dirawa...

Major differences between healthy peri-implant & p...

The implant device lacks tooth characteristic structures such as root cementum, periodontal ligament, and bundle bone (alveolar bone proper). The dento‐alveolar and the dento‐gingival fiber bundles connect the soft tissues with the tooth (root cementum), while no such fiber bundles are apparent in the peri‐implant tissues. At periodontally healthy sites, the margin of the gingiva follow...

Bone tissue around implants

Bone tissue in the edentulous ridge In a study involving partially edentulous subjects, hard tissue biopsies were sampled from the maxilla and the mandible with the use of trephine drills. The bone tissue was found to include a blend of mainly lamellar bone (46%) and bone marrow (23%) with less amounts of fibrous (12%) and osteoid (4%) tissue. Bone marrow was the dominant tissu...

Keratinized mucosa : peri-implant health

KERATINIZED MUCOSA (KM) Keratinized mucosa is a term used to describe the masticatory mucosa that is present at many, but not all, implant sites. Keratinized mucosa extends from the margin of the peri‐implant mucosa to the movable lining (oral) mucosa. Keratinized mucosa is comprised of a lamina propria (fibrous connective tissue that contains fibroblasts and equal amounts of ty...

Bone sounding : peri-implant health

BONE SOUNDING Bone sounding or transmucosal sounding (TS) is a measurement that is used to determine the height of the entire soft tissue cuff at various groups of teeth and implants. The dimensions of the peri‐implant mucosa and the gingiva at adjacent tooth sites was studied by clinical measurements performed mainly in partially edentulous subjects who had been treated with im...

Peri-implant tissues in clinical health : peri-imp...

PERI‐IMPLANT TISSUES IN CLINICAL HEALTH The gingiva and the peri‐implant mucosa and their adhesion (seal) are consistently challenged by the oral environment, including the steady exposure to microorganisms in the biofilm present on the tooth and implant surfaces. In the clinically normal peri‐implant mucosa (and gingiva), the continuous host response includes both vascular ...

Dimension of the peri-implant mucosa : peri-implan...

Animal studies The dimension of the peri‐implant mucosa, often called the biological width or dimension, was examined in biopsies mainly obtained from studies in dogs. Such measurements disclosed that a certain width of soft tissue may be required to cover the peri‐implant bone. The studies referred to the length of the epithelium (from the peri‐implant mucosa margin to the...

Morphogenesis of the mucosal adhesion : peri-impla...

The formation of the mucosal adhesion was studied in a dog model. One‐piece implant devices were placed in the edentulous mandible of dogs, and healing was monitored using light microscopic examination of biopsies sampled at different intervals during a 3‐month period. In the initial phase of the wound between the implant and cut connective tissue, a fibrin clot/coagulum formed that was inf...

Peri‐implant mucosa: peri-implant health

PERI‐IMPLANT MUCOSA Most information regarding the structural features of the peri‐implant mucosa is derived from animal studies using dog models. In such studies implants were placed in the edentulous ridge (alternatively, the fresh extraction socket), the outer osseous part of which was covered with masticatory mucosa. It was also shown that the healed peri‐implant mucosa on the buccal a...

Introduction: Peri‐implant health

Peri‐implant tissues are those that occur around osseointegrated dental implants. They are divided into soft and hard tissue compartments. The soft tissue compartment is denoted “peri‐implant mucosa” and is formed during the wound healing process that follows implant/abutment placement. The hard tissue compartment forms a contact relationship to the implant surface to secure implant stab...

Peri‐implant health

Abstract Objective The aim is to define clinical and histologic characteristics of peri‐implant tissues in health and describe the mucosa–implant interface. Importance An understanding of the characteristics of healthy peri‐implant tissues facilitates the recognition of disease (i.e., departure from health). Findings The healthy peri‐implant mucosa is, at the microscopic ...

Ridge alterations: 4 week

Figure 48. Dimensional ridge alterations: 4 weeks At 4 weeks after tooth extraction, the socket is filled with woven bone. Osteoclasts are present on the outer surfaces at the margin of the buccal and lingual walls, signaling resorption of cortical plates. The resorption of the bundle bone is almost complete. Osteoclasts also line the trabeculae of woven bone present in the central and latera...

Ridge alterations: 2 week

Figure 47. Dimensional ridge alterations: 2 weeks At 2 weeks after tooth extraction, the apical and lateral parts of the socket are filled with woven bone, while the central and marginal portions of the socket are occupied by provisional connective tissue. On the inner and outer surfaces of the socket walls, numerous osteoclasts can be seen. In several areas of the socket wall, the bundle bon...

Day 4 - 14 of soft tissue healing: epithelial prol...

Figure 34. Day 4 - 14 : Epithelial proliferation & connective tissue After 4 to 5 days, the epithelium from the margins of the soft tissue starts to proliferate to cover the granulation tissue in the socket. At 14 days, the connective tissue at the marginal portion of the extraction socket is partially lined by epithelial cells.

Quantitative tissue analysis

Figure 30. Quantitative tissue analysis Trombelli and coworkers monitored the healing of human extraction sockets for a 6-month period and presented a semi-quantitative analysis of tissues and cell populations involved in various stages of socket healing. They showed that granulation tissue was present in comparatively large amounts in the early phases of socket healing. At 6 to 8 weeks, th...

Day 14 : Woven bone & connective tissue

Figure 26. Day 14: woven bone & connective tissue After 14 days of healing woven bone, which appears as a loose, unstructured network of bone, has started to fill the socket except in the central region, where significant amounts of the provisional connective tissue matrix still remain. This is because the woven bone forms first at the periphery of the socket and gradually extends from the wa...