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Fig. 4. a Peri-apical X-ray of zirconia scaffold i...

Fig. 4. e. Margins between bone defect and scaffold are less demarcated due to new bone growth Fig. 4. a Peri-apical X-ray of zirconia scaffold immediately placed in bone defect. Margins between scaffold and bone are clearly demarcated. b Peri-apical X-ray of zirconia scaffold after completion of healing time. Margins between bone defect and scaffold are less demarcated due to new bone growth

Fig. 3. a Histological section demonstrating bone ...

Fig. 3. hite arrow). Mineralized bone formation (black arrow) was less dense compared to HA-enriched scaffolds. b Histological section showing different sizes of pores present in porous zirconia scaffolds (Control specimen). Mineralization started by lining pore walls (white arrow). Unmineralized bone stained blue Fig. 3. a Histological section demonstrating bone growth in control zirconia sc...

Fig. 2. a Histological section demonstrating new b...

Fig. 2. zirconia scaffold (black arrow). Unmineralized bone stained blue. Almost entire surface porosity was filled with new dense bone. b Histological section demonstrating bone growth in HA-enriched zirconia scaffold starting from the periphery of the surgical wound (white arrow). Islands of entrapped HA particles were surrounded by mineralized boney matrix (black arrow) which were identified ...

Fig. 1. a SEM image, ×10,000, demonstrating inter...

Fig. 1. rating agglomeration of nano-hydroxyapatite particles filling the porous structure Fig. 1. a SEM image, ×10,000, demonstrating internal porosity of the fabricated zirconia scaffolds. b SEM image, ×30,500, demonstrating agglomeration of nano-hydroxyapatite particles filling the porous structure

About this article : Osteogenesis ability of CAD/C...

Aboushelib, M.N., Shawky, R. Osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with nano-hydroxyapatite particles. Int J Implant Dent 3, 21 (2017). https://doi.org/10.1186/s40729-017-0082-6 Download citation Received: 15 November 2016 Accepted: 08 May 2017 Published: 19 May 2017 DOI: https://doi.org/10.1186/s40729-017-0082-6

Rights and permissions : Osteogenesis ability of C...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...

Author information : Osteogenesis ability of CAD/C...

Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Champollion st, Azarita, Alexandria, Egypt Moustafa N. Aboushelib Oral Surgery Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt Rehab Shawky You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar Correspondence to Moustafa...

Acknowledgements : Osteogenesis ability of CAD/CAM...

Part of this study was supported by STDF reintegration grant number 489 and was performed in collaboration with the Oral Surgery Department, Faculty of Dentistry, Alexandria University, Egypt. MNA prepared the specimens and performed the characterization process. He participated in preparation of histological section, data collection and analysis, and in preparation of the manuscript. RS performe...

References : Osteogenesis ability of CAD/CAM porou...

Naleway SE, Fickas KC, Maker YN, Meyers MA, McKittrick J. Reproducibility of ZrO2-based freeze casting for biomaterials. Mater Sci Eng C Mater Biol Appl. 2016;61:105–12. Song YG, Cho IH. Characteristics and osteogenic effect of zirconia porous scaffold coated with beta-TCP/HA. J Adv Prosthodont. 2014;6:285–94. Okano T, et al. Absorbed and effective doses from cone beam volumetric imaging for...

References : Osteogenesis ability of CAD/CAM porou...

Chiapasco M, Casentini P, Zaniboni M. Bone augmentation procedures in implant dentistry. Int J Oral Maxillofac Implants. 2009;24:237–59. Rocchietta I, Fontana F, Simion M. Clinical outcomes of vertical bone augmentation to enable dental implant placement: a systematic review. J Clin Periodontol. 2008;35:203–15. Louis PJ, Gutta R, Said-Al-Naief N, Bartolucci AA. Reconstruction of the maxilla ...

Conclusions : Osteogenesis ability of CAD/CAM poro...

Within the limitations of this study, hydroxyapatite enhanced osteogenesis ability of porous zirconia scaffolds.

Discussion : Osteogenesis ability of CAD/CAM porou...

Histomorphometric analysis revealed that bone growth start to develop as early as 6 weeks by lining pore cavity walls. Mineralized bone matrix was observed to penetrate 1–2 mm under the surface of the scaffolds thus providing mechanical stability of the inserted prosthesis. Healing continued by filling the entire pore volume (Fig. 2a, b). The presence of nano-hydroxyapatite particles enhanced...

Discussion : Osteogenesis ability of CAD/CAM porou...

Porous scaffolds are designed to allow ingrowth of the surrounding bone within the internal porosity of the solid matrix. Different types of bioactive materials were mixed with zirconia to enhance bone formation. Two sizes of pores were incorporated in the structure of the fabricated scaffolds. Micro-pores in range of 50 μm constituted the majority of the entire pore volume (50 wt.%) of the fabr...

Results : Osteogenesis ability of CAD/CAM porous z...

Mercury porosimetery revealed comparable (F = 0.057, P 

Methods : Osteogenesis ability of CAD/CAM porous z...

Six weeks after insertion of the scaffolds, the animals were given an over dose of an anesthetic injection and section blocks were obtained by cutting the mandible maintaining 10 mm of sound bone around the scaffolds. Cut sections were immediately fixed in 4% buffered formaldehyde and dehydrated in graded ethanol solutions using a dehydration system under agitation and vacuum, and the specimens w...

Methods : Osteogenesis ability of CAD/CAM porous z...

Nano-hydroxyapatite particles were prepared using sol gel chemical precipitation method. The sol was thermally aged at low temperature at 50 °C for 2 h. Upon drying the sol particles agglomerated into a dry gel through van der Waals forces composed of 10–14-nm particles. A crystalline apatite is achieved after sintering at 450 °C resulting in a gained structure of 25–55 nm in diameter. T...

Methods : Osteogenesis ability of CAD/CAM porous z...

Zirconia powder (50 μm, 3 mol YTZP, E grade, Tosoh, Japan) was mixed with 50 wt.% resin beads (50 μm polymethyl methacrylate powder) added to create microscopic pore sizes. Thirty weight percent coarse sodium chloride particles (500–700 μm) were added to the mix to create large interconnected pores. The powder was mixed in a rotating cylinder for 24 h to insure homogenous powder distribu...

Background : Osteogenesis ability of CAD/CAM porou...

Modern radiographic imaging techniques in combination with advanced computer designing software could reconstruct a three-dimensional image of large boney defects [16]. Designing the shape of the required scaffold could easily be performed to accurately fit the available defect size using advanced imaging and designing software [17]. Finally, the required shape could be directly milled from differ...

Background : Osteogenesis ability of CAD/CAM porou...

Principles of tissue engineering are used today in an attempt to reconstruct damaged human tissue. In the dental field, several types of bone grafting materials are currently available which could be directly used to augment atrophic jaws before implant placement. However, the main drawback of these grafts is related to difficulty of preserving the required shape of the graft during the healing ti...

Abstract : Osteogenesis ability of CAD/CAM porous ...

The aim of this study was to evaluate osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with hydroxy apatite used to augment large boney defects in a dog model. Surgical defects were made bilaterally on the lower jaw of 12 Beagle dogs. Cone beam CT images were used to create three dimensional images of the healed defects. Porous zirconia scaffolds were fabricated by milling custo...

About this article : Osteogenesis ability of CAD/C...

Aboushelib, M.N., Shawky, R. Osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with nano-hydroxyapatite particles. Int J Implant Dent 3, 21 (2017). https://doi.org/10.1186/s40729-017-0082-6 Download citation Received: 15 November 2016 Accepted: 08 May 2017 Published: 19 May 2017 DOI: https://doi.org/10.1186/s40729-017-0082-6

Rights and permissions : Osteogenesis ability of C...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...

Author information : Osteogenesis ability of CAD/C...

Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Champollion st, Azarita, Alexandria, Egypt Moustafa N. Aboushelib Oral Surgery Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt Rehab Shawky You can also search for this author in PubMed Google Scholar You can also search for this author in ...

Acknowledgements : Osteogenesis ability of CAD/CAM...

Part of this study was supported by STDF reintegration grant number 489 and was performed in collaboration with the Oral Surgery Department, Faculty of Dentistry, Alexandria University, Egypt. MNA prepared the specimens and performed the characterization process. He participated in preparation of histological section, data collection and analysis, and in preparation of the manuscript. RS performe...

References : Osteogenesis ability of CAD/CAM porou...

Naleway SE, Fickas KC, Maker YN, Meyers MA, McKittrick J. Reproducibility of ZrO2-based freeze casting for biomaterials. Mater Sci Eng C Mater Biol Appl. 2016;61:105–12. Song YG, Cho IH. Characteristics and osteogenic effect of zirconia porous scaffold coated with beta-TCP/HA. J Adv Prosthodont. 2014;6:285–94. Okano T, et al. Absorbed and effective doses from cone beam volumetric imaging for...

References : Osteogenesis ability of CAD/CAM porou...

Chiapasco M, Casentini P, Zaniboni M. Bone augmentation procedures in implant dentistry. Int J Oral Maxillofac Implants. 2009;24:237–59. Rocchietta I, Fontana F, Simion M. Clinical outcomes of vertical bone augmentation to enable dental implant placement: a systematic review. J Clin Periodontol. 2008;35:203–15. Louis PJ, Gutta R, Said-Al-Naief N, Bartolucci AA. Reconstruction of the maxilla ...

Conclusions : Osteogenesis ability of CAD/CAM poro...

Within the limitations of this study, hydroxyapatite enhanced osteogenesis ability of porous zirconia scaffolds.

Discussion : Osteogenesis ability of CAD/CAM porou...

Histomorphometric analysis revealed that bone growth start to develop as early as 6 weeks by lining pore cavity walls. Mineralized bone matrix was observed to penetrate 1–2 mm under the surface of the scaffolds thus providing mechanical stability of the inserted prosthesis. Healing continued by filling the entire pore volume (Fig. 2a, b). The presence of nano-hydroxyapatite particles enhanced...

Discussion : Osteogenesis ability of CAD/CAM porou...

Porous scaffolds are designed to allow ingrowth of the surrounding bone within the internal porosity of the solid matrix. Different types of bioactive materials were mixed with zirconia to enhance bone formation. Two sizes of pores were incorporated in the structure of the fabricated scaffolds. Micro-pores in range of 50 μm constituted the majority of the entire pore volume (50 wt.%) of the fabr...

Results : Osteogenesis ability of CAD/CAM porous z...

Mercury porosimetery revealed comparable (F = 0.057, P 

Methods : Osteogenesis ability of CAD/CAM porous z...

Six weeks after insertion of the scaffolds, the animals were given an over dose of an anesthetic injection and section blocks were obtained by cutting the mandible maintaining 10 mm of sound bone around the scaffolds. Cut sections were immediately fixed in 4% buffered formaldehyde and dehydrated in graded ethanol solutions using a dehydration system under agitation and vacuum, and the specimens w...

Methods : Osteogenesis ability of CAD/CAM porous z...

Nano-hydroxyapatite particles were prepared using sol gel chemical precipitation method. The sol was thermally aged at low temperature at 50 °C for 2 h. Upon drying the sol particles agglomerated into a dry gel through van der Waals forces composed of 10–14-nm particles. A crystalline apatite is achieved after sintering at 450 °C resulting in a gained structure of 25–55 nm in diameter. T...

Methods : Osteogenesis ability of CAD/CAM porous z...

Zirconia powder (50 μm, 3 mol YTZP, E grade, Tosoh, Japan) was mixed with 50 wt.% resin beads (50 μm polymethyl methacrylate powder) added to create microscopic pore sizes. Thirty weight percent coarse sodium chloride particles (500–700 μm) were added to the mix to create large interconnected pores. The powder was mixed in a rotating cylinder for 24 h to insure homogenous powder distribu...

Background : Osteogenesis ability of CAD/CAM porou...

Modern radiographic imaging techniques in combination with advanced computer designing software could reconstruct a three-dimensional image of large boney defects [16]. Designing the shape of the required scaffold could easily be performed to accurately fit the available defect size using advanced imaging and designing software [17]. Finally, the required shape could be directly milled from differ...

Background : Osteogenesis ability of CAD/CAM porou...

Principles of tissue engineering are used today in an attempt to reconstruct damaged human tissue. In the dental field, several types of bone grafting materials are currently available which could be directly used to augment atrophic jaws before implant placement. However, the main drawback of these grafts is related to difficulty of preserving the required shape of the graft during the healing ti...

Abstract : Osteogenesis ability of CAD/CAM porous ...

The aim of this study was to evaluate osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with hydroxy apatite used to augment large boney defects in a dog model. Surgical defects were made bilaterally on the lower jaw of 12 Beagle dogs. Cone beam CT images were used to create three dimensional images of the healed defects. Porous zirconia scaffolds were fabricated by milling custo...

About this article : Osteogenesis ability of CAD/C...

Aboushelib, M.N., Shawky, R. Osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with nano-hydroxyapatite particles. Int J Implant Dent 3, 21 (2017). https://doi.org/10.1186/s40729-017-0082-6 Download citation Received: 15 November 2016 Accepted: 08 May 2017 Published: 19 May 2017 DOI: https://doi.org/10.1186/s40729-017-0082-6

Rights and permissions : Osteogenesis ability of C...

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...

Author information : Osteogenesis ability of CAD/C...

Dental Biomaterials Department, Faculty of Dentistry, Alexandria University, Champollion st, Azarita, Alexandria, Egypt Moustafa N. Aboushelib Oral Surgery Department, Faculty of Dentistry, Alexandria University, Alexandria, Egypt Rehab Shawky You can also search for this author in PubMed Google Scholar You can also search for this author in ...

Acknowledgements : Osteogenesis ability of CAD/CAM...

Part of this study was supported by STDF reintegration grant number 489 and was performed in collaboration with the Oral Surgery Department, Faculty of Dentistry, Alexandria University, Egypt. MNA prepared the specimens and performed the characterization process. He participated in preparation of histological section, data collection and analysis, and in preparation of the manuscript. RS performe...

References : Osteogenesis ability of CAD/CAM porou...

Naleway SE, Fickas KC, Maker YN, Meyers MA, McKittrick J. Reproducibility of ZrO2-based freeze casting for biomaterials. Mater Sci Eng C Mater Biol Appl. 2016;61:105–12. Song YG, Cho IH. Characteristics and osteogenic effect of zirconia porous scaffold coated with beta-TCP/HA. J Adv Prosthodont. 2014;6:285–94. Okano T, et al. Absorbed and effective doses from cone beam volumetric imaging for...

References : Osteogenesis ability of CAD/CAM porou...

Chiapasco M, Casentini P, Zaniboni M. Bone augmentation procedures in implant dentistry. Int J Oral Maxillofac Implants. 2009;24:237–59. Rocchietta I, Fontana F, Simion M. Clinical outcomes of vertical bone augmentation to enable dental implant placement: a systematic review. J Clin Periodontol. 2008;35:203–15. Louis PJ, Gutta R, Said-Al-Naief N, Bartolucci AA. Reconstruction of the maxilla ...

Conclusions : Osteogenesis ability of CAD/CAM poro...

Within the limitations of this study, hydroxyapatite enhanced osteogenesis ability of porous zirconia scaffolds.

Discussion : Osteogenesis ability of CAD/CAM porou...

Histomorphometric analysis revealed that bone growth start to develop as early as 6 weeks by lining pore cavity walls. Mineralized bone matrix was observed to penetrate 1–2 mm under the surface of the scaffolds thus providing mechanical stability of the inserted prosthesis. Healing continued by filling the entire pore volume (Fig. 2a, b). The presence of nano-hydroxyapatite particles enhanced...

Discussion : Osteogenesis ability of CAD/CAM porou...

Porous scaffolds are designed to allow ingrowth of the surrounding bone within the internal porosity of the solid matrix. Different types of bioactive materials were mixed with zirconia to enhance bone formation. Two sizes of pores were incorporated in the structure of the fabricated scaffolds. Micro-pores in range of 50 μm constituted the majority of the entire pore volume (50 wt.%) of the fabr...

Results : Osteogenesis ability of CAD/CAM porous z...

Mercury porosimetery revealed comparable (F = 0.057, P 

Methods : Osteogenesis ability of CAD/CAM porous z...

Six weeks after insertion of the scaffolds, the animals were given an over dose of an anesthetic injection and section blocks were obtained by cutting the mandible maintaining 10 mm of sound bone around the scaffolds. Cut sections were immediately fixed in 4% buffered formaldehyde and dehydrated in graded ethanol solutions using a dehydration system under agitation and vacuum, and the specimens w...

Methods : Osteogenesis ability of CAD/CAM porous z...

Nano-hydroxyapatite particles were prepared using sol gel chemical precipitation method. The sol was thermally aged at low temperature at 50 °C for 2 h. Upon drying the sol particles agglomerated into a dry gel through van der Waals forces composed of 10–14-nm particles. A crystalline apatite is achieved after sintering at 450 °C resulting in a gained structure of 25–55 nm in diameter. T...

Methods : Osteogenesis ability of CAD/CAM porous z...

Zirconia powder (50 μm, 3 mol YTZP, E grade, Tosoh, Japan) was mixed with 50 wt.% resin beads (50 μm polymethyl methacrylate powder) added to create microscopic pore sizes. Thirty weight percent coarse sodium chloride particles (500–700 μm) were added to the mix to create large interconnected pores. The powder was mixed in a rotating cylinder for 24 h to insure homogenous powder distribu...

Background : Osteogenesis ability of CAD/CAM porou...

Modern radiographic imaging techniques in combination with advanced computer designing software could reconstruct a three-dimensional image of large boney defects [16]. Designing the shape of the required scaffold could easily be performed to accurately fit the available defect size using advanced imaging and designing software [17]. Finally, the required shape could be directly milled from differ...

Background : Osteogenesis ability of CAD/CAM porou...

Principles of tissue engineering are used today in an attempt to reconstruct damaged human tissue. In the dental field, several types of bone grafting materials are currently available which could be directly used to augment atrophic jaws before implant placement. However, the main drawback of these grafts is related to difficulty of preserving the required shape of the graft during the healing ti...

Abstract : Osteogenesis ability of CAD/CAM porous ...

The aim of this study was to evaluate osteogenesis ability of CAD/CAM porous zirconia scaffolds enriched with hydroxy apatite used to augment large boney defects in a dog model. Surgical defects were made bilaterally on the lower jaw of 12 Beagle dogs. Cone beam CT images were used to create three dimensional images of the healed defects. Porous zirconia scaffolds were fabricated by milling custo...

Conclusions: Porous collagen-hydroxyapatite scaffo...

Conclusions The porous Col-HA composites developed in the present study are biocompatible and can be used as scaffolds for bone tissue regeneration. The Col-HA ratio is an important factor in promoting the attachment and proliferation of mouse MSCs. The Col-HA composite complexes have strong potentials in bone tissue regeneration applications. hPDSCs may be a suitable resource of cells for maxi...

Discussion: Porous collagen-hydroxyapatite scaffol...

Discussion The findings of the presented study indicate that the porous sponge-like Col-HA composites have good biocompatibility and biomimetic properties and may be used as scaffolds for bone tissue regeneration. The Col-HA composites with ratios 80:20 and 50:50 supported the attachments and proliferations of mouse MSCs and hPDSCs. These findings indicate that Col-HA composite complexes have str...

Results: Porous collagen-hydroxyapatite scaffolds ...

Results The sponge-like plugs of prototype Col-HA composites were successfully fabricated with different collagen and HA ratios. The macroscopic and SEM views of the prototype type I collagen without HA and 3 different ratios of collagen-HA (20%Col-80%HA; 50%Col-50%HA; 80%Col-20%HA) composites are shown in Figure 1. The SEM views show the inside microstructures of the prototype pure type I colla...

Materials & methods: Porous collagen-hydroxyapatit...

Materials and Methods Synthesis of the Col-HA composites by direct precipitation in situ Solutions of calcium salt and phosphoric acid (Ca/P = 1.66 mol) were used to synthesize HA particles and incorporate them on bovine type I collagen fibrils by a direct precipitation technique in situ. This technique was optimized to produce 3 different ratios of Col-HA composites (20%Col-80%HA; 50%Col-50%H...

Introduction: Porous collagen-hydroxyapatite scaff...

Introduction Combining a scaffold and living cells to form a tissue-engineering construct is an important concept for promoting the repair and regeneration of bone tissues. Mesenchymal stem cells are often used in such constructs due to their abilities to proliferate and differentiate toward bone-forming cells. The design and fabrication of scaffolds, stem cell isolation and characterization, and...

Abstract: Porous collagen-hydroxyapatite scaffolds...

Abstract Current bone grafting materials have significant limitations for repairing maxillofacial and dentoalveolar bone deficiencies. An ideal bone tissue-engineering construct is still lacking. The purpose of the present study was first to synthesize and develop a collagen-hydroxyapatite (Col-HA) composite through controlled in situ mineralization on type I collagen fibrils with nanometer-sized...

Porous collagen-hydroxyapatite scaffolds with mese...

  RESEARCH Porous Collagen-Hydroxyapatite Scaffolds With Mesenchymal Stem Cells for Bone Regeneration Li Ning, DDS, PhD , Hans Malmström, DDS , Yan-Fang Ren, DDS, MPH, PhD Correspondence: * Corresponding author, e-mail: yanfang_ren@urmc.rochester.edu Article Citation: Li Ning, Hans Malmström, Yan-Fang Ren, Porous Collagen-Hydroxyapatite Scaffolds With Mesench...