Figure 6. Control group. Some implant thread areas were not covered by bone layer (magnification × 25—toluidine blue)
Figure 6. Control group. Some implant thread areas were not covered by bone layer (magnification × 25—toluidine blue)
Figure 5. Control group. Implants belonging to the control group showed some small surface areas not contacted with bone (magnification × 25—toluidine blue)
Figure 5. Control group. Implants belonging to the control group showed some small surface areas not contacted with bone (magnification × 25—toluidine blue)
Figure 4. Control group. No bone condensation was possible with traditional burs and standard implant (magnification × 25—toluidine blue)
Figure 4. Control group. No bone condensation was possible with traditional burs and standard implant (magnification × 25—toluidine blue)
Figure 3. Test group. The present histological photo showed a continuous thin layer of newly formed bone along the neck area of the implant (magnification × 25—toluidine blue)
Figure 3. Test group. The present histological photo showed a continuous thin layer of newly formed bone along the neck area of the implant (magnification × 25—toluidine blue)
Figure 2. Test group. Implants in the test group showed an extremely high percentage of bone directly contacted to implant surface (magnification × 25—toluidine blue)
Figure 2. Test group. Implants in the test group showed an extremely high percentage of bone directly contacted to implant surface (magnification × 25—toluidine blue)
Figure 1. Test group. The implant achieved a high osseointegration degree. The newly formed bone appeared well interconnected with the pre-existing trabeculae. The “corticalization” phenomenon is evident: the bone appears densified around a titanium implant (magnification × 8—toluidine blue)
Figure 1. Test group. The implant achieved a high osseointegration degree. The newly formed b...
BIC%P < 0.05BV%P < 0.05VamP < 0.05Reverse torqueP > 0.05 NsISQP < 0.05Table 3 Statistical comparison (T test) of examined parameters between the test and control groups. The histomorphometric analysis demonstrated significant differences in BIC% and %BV values between the two implant groups
Implant typeBIC%BV%Vam (μm) ± SDReverse torque (N/cm) ± SDISQ value ± SDTest group70.91 ± 7.9541.83 ± 6.3082.6 ± 23.2798.2 ± 16.8163.5 ± 1.30Control group49.33 ± 10.7329.61 ± 5.0560.5 ± 16.5898.8 ± 24.4059.4 ± 1.39Table 2 Mean values of histomorphometric parameters (%BIC and %BV) and biomechanical values (VAM, reverse torque, and ISQ) of each implant group
Basal %BV 26.17 ± 2.35Test groupControl group 41.83 ± 6.30*29.61 ± 5.05Table 1 Basal bone volume percentage (basal %BV) was compared to %BV around implants after 2 months of healing in both groups. %BV in the test group was significantly higher than basal %BV (P < 0.05)
Trisi, P., Falco, A. & Berardini, M. Single-drill implant induces bone corticalization during submerged healing: an in vivo pilot study. Int J Implant Dent 6, 2 (2020). https://doi.org/10.1186/s40729-019-0198-y
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Received: 30 August 2019
Accepted: 04 December 2019
Published: 15 January 2020
DOI: https://doi.org/10.1186/s40729-019-0198-y
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were m...
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
The Ethics Committee for Animal Research of the Veterinary School of the University of Teramo (Teramo, Italy) approved the study protocol with the protocol number 8110.
The Ethics Committee for Animal Research of the Veterinary School of the University of Teramo (Teramo, Italy) approved the study protocol with the protocol number 8110.
The authors declare that they have no competing interests.
Biomaterial Clinical and Histological Research Association, Private Practice, Via Galilei 8, 65122, Pescara, Italy
Paolo Trisi, Antonello Falco & Marco Berardini
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PT contributed to the study design, animal s...
No grants were received for the present study.
The authors wish to thank Cortex® Dental Implants (Shlomi, Israel) and NoDrill® (Milano, Italy) for providing the implants used in the present study.
Gehrke SA, Bettach R, Aramburú Júnior JS, Prados-Frutos JC, Del Fabbro M, Shibli JA. Peri-Implant Bone Behavior after Single Drill versus Multiple Sequence for Osteotomy Drill. Biomed Res Int. 2018;11:2018.
Möhlhenrich SC, Abouridouane M, Heussen N, Hölzle F, Klocke F, Modabber A. Thermal evaluation by infrared measurement of implant site preparation between single and gradual drilling in art...
Summers RB. A new concept in maxillary implant surgery: the osteotome technique. Compendium. 1994;15:152–6.
Büchter A, Kleinheinz J, Wiesmann HP, et al. Biological and biomechanical evaluation of bone remodelling and implant stability after using an osteotome technique. Clin Oral Implants Res. 2005;16:1–8.
Stavropoulos A, Nyengaard JR, Lang NP, et al. Immediate loading of single SLA implant...
Albrektsson T, Brånemark PI, Hansson HA, et al. Osseointegrated titanium implants. Requirements for ensuring a long-lasting, direct bone-to-implant anchorage in man. Acta Orthop Scand. 1981;52(2):155–70.
Molly L. Bone density and primary stability in implant therapy. Clin Oral Implants Res. 2006;2:124–35.
Chong L, Khocht A, Suzuki JB, et al. Effect of implant design on initial stability of ...
All data and materials are available from the corresponding author in Pescara, Italy.
The hypothesis was accepted. Compressing implants with single-drill bone preparation demonstrated many clinical and histological advantages with respect with to standard implant drilling procedures and classical implant shape and design in low-density bone type. It is possible to summarize the advantages of this technique as follows: higher bone to implant contact percentage than the control group...
The comparison between the basal %BV and %BV in the test group revealed that these innovative implants were able to increase in a significant way the peri-implant bone density with respect to starting host bone density. This increased peri-implant bone density extended from about 0.5–0.7 mm beyond the implant perimeter causing an osseocorticalization around the fixture profile.
A recent study [...
Results from the present study clearly show that it is possible to insert an implant using a one-step concept for the surgical preparation of the bone bed in cancellous bone.
Guazzi et al. [24], comparing the clinical outcome of implants inserted in sites prepared with a simplified protocol consisting of one large single drill versus multiple conventional drilling steps, demonstrated less surgica...
No implant failure was observed after 2 months of healing. The clinical examination, done immediately after the bone block retrieval, showed no crestal bone resorption. No bone defects around implants, such as fenestration or dehiscence, were detected. The host bone density expressed in bone volume percentage (basal %BV) was 26.17 ± 2.35. This low value of BV% is common in soft bone, according to...
Specimens were immediately fixed in 10% neutral buffered formalin and processed for histologic analysis. After dehydration, samples were infiltrated with a methyl-methacrylate resin from a starting solution 50% ethanol/resin and subsequently 100% resin, with each step lasting 24 h. After polymerization, the blocks were sectioned and then ground down to about 40 μm. Toluidine blue staining was use...
After implant insertion, cover screws were secured and the surgical wounds were closed by a resorbable periosteal-muscular inner suture, followed by an external cutaneous 2-0 silk suture.
Each animal underwent systemic antibiotic therapy for 5 days with 8 ml long-acting Clamoxil (Pfizer Limited, Sandwich, USA). After surgery, animals received appropriate veterinary care and were allowed free acce...
The Ethics Committee for Animal Research of the Veterinary School of the University of Teramo (Teramo, Italy) approved the study protocol, which followed the guidelines established by the European Union Council Directive of February 2013 (R.D.53/2013).
Two female sheep, 4–5 years old, were included in the study. Clinical examination determined that all animals were in good general health. Exclu...
The aim of the present paper is to evaluate a reduced implant site preparation technique to preserve bone bulk and enhance primary stability using a new conical self-tapping implant in cancellous type IV bone. A histologic, histomorphometric, and biomechanical comparative analysis was conducted in low-density bone of the sheep’s iliac crest. The hypothesis to test is if undersized preparation an...
It is well documented that implant initial bone fixation, known as primary implant stability, represents the pre-requisite to achieve a successful long-term osseointegration [1].
Many studies demonstrated that the implant primary stability is strictly influenced by host bone density [2], fixture geometry [3, 4], and surgical technique used for preparing bone implant bed [5]. Other studies [6, 7] ...
The aim of the present paper is to evaluate a simplified implant site preparation technique to preserve bone bulk and enhance osseointegration using a new conical self-tapping implant in cancellous bone.
Ten Expander® 3.8 × 10 mm implants (NoDrill®, Milano, Italy) were inserted in the right side (test group) of sheep’s iliac crest using only the pilot drill 1.8 mm in diameter. Ten 3.8 × 10 ...
Fig. 7 Second stage surgery of patient in Fig. 1. a Vestibular depth reduction after augmentation and implant placement. b Partial thickness and apical repositioned flap. c CMX healing and soft tissue dehiscence with CCXBB exposure. d Dehiscence healing after re-contouring and buccal emergency profile. e Buccal aspect of the final restoration. f Buccal ridge contour
Fig. 6 Immunohistochemical analysis of slices from the same sample with four different markers. a TRAP. b OPN. c ALP. d OSC
ple. a Ground section stained with Levai-Laczkó. b Tissue identification of the ROI. c Closer view a bone and CCXBB. d Closer view of b
Fig. 5. Histomorphometric analysis of the same sample. a Ground section stained with Levai-Laczkó. b Tissue identification of the ROI. c Closer view a arrow pointing a cement line between new mineralized bone and CCXBB. d Closer view of b
Fig. 4. Histological samples. a CCXBB control without implantation. b Histologic samples with acute inflammatory infiltration. c Histologic sample with limited remaining CCXBB and large bone ingrowth
Fig. 4. Histological samples. a CCXBB control without implantation. b Histologic samples with acute inflammatory infiltration. c Histologic sample with limited remaining CCXBB and large bone ingr...
Fig. 3. Re-entry procedure of patient in Fig. 1. a Buccal aspect of the augmented region. b Horizontal bone augmentation. c Screws and pins removal and bone trephine sampling. d Implants placement and buccal bone width from the implant shoulder. e Primary flap closure. f Implants submerged healing
Fig. 3. Re-entry procedure of patient in Fig. 1. a Buccal aspect of the augmented region. b Ho...
Fig. 2. Lateral bone augmentation of the alveolar crest (a) atrophic ridge. b Perforations and adaptation of the cortical layer. c Shaping, pre-wetting and fixation of CCXBB with titanium screws. d Horizontal contour and peripheral gap between CCXBB and bone layer. e Outlying DBBM filling. f CM stabilized with pins
Fig. 2. Lateral bone augmentation of the alveolar crest (a) atrophic ridge. b ...
Fig. 1. Study chart and follow-up visits
Fig. 1. Study chart and follow-up visits
Differentiated tissues
Implant lost (Yes/no)
Mean
SD
Percentage
SD (%)
...
Patient
TRAP (%)
OPN (%)
ALP (%)
OSC (%)
1
...
Tissue type
Mean
Standard deviation
Median
CI 95%
Mineralized bone
...
Patient
Soft tissue dehiscence
Mineralized bone (%)
CCXBB (%)
Bone marrow (%)
Connect...
Ortiz-Vigón, A., Martinez-Villa, S., Suarez, I. et al. Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement.
Int J Implant Dent 3, 24 (2017). https://doi.org/10.1186/s40729-017-0087-1
Download citation
Received: 21 March 2017
Accepted: 12 June 2017
Published: 21 Ju...
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...
ETEP Research Group, Facultad de Odontología, Universidad Complutense de Madrid, Plaza Ramón y Cajal, 28040, Madrid, Spain
Alberto Ortiz-Vigón, Sergio Martinez-Villa, Iñaki Suarez, Fabio Vignoletti & Mariano Sanz
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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
We wish to acknowledge the dedication and scientific advise of Prof. Dr. Tord Berglundh on the histological analysis as well as the diligent work in processing the histological samples to Estela Maldonado for the immunohistochemistry and Asal Shikhan and Fernando Muñoz for the histomorphometry. The work of Esperanza Gross on the statistical analysis is highly acknowledged.
This study was partial...
Patti A, Gennari L, Merlotti D, Dotta F, Nuti R. Endocrine actions of osteocalcin. Int J Endocrinol. 2013;2013:846480.
Schwarz F, Herten M, Sager M, Wieland M, Dard M, Becker J. Histological and immunohistochemical analysis of initial and early osseous integration at chemically modified and conventional SLA titanium implants: preliminary results of a pilot study in dogs. Clin Oral Implants Res. 2...
Araujo MG, Linder E, Lindhe J. Bio-Oss collagen in the buccal gap at immediate implants: a 6-month study in the dog. Clin Oral Implants Res. 2011;22:1–8.
Chiapasco M, Colletti G, Coggiola A, Di Martino G, Anello T, Romeo E. Clinical outcome of the use of fresh frozen allogeneic bone grafts for the reconstruction of severely resorbed alveolar ridges: preliminary results of a prospective study. I...
Jeno L, Geza L. A simple differential staining method for semi-thin sections of ossifying cartilage and bone tissues embedded in epoxy resin. Mikroskopie. 1975;31:1–4.
Dias RR, Sehn FP, de Santana Santos T, Silva ER, Chaushu G, Xavier SP. Corticocancellous fresh-frozen allograft bone blocks for augmenting atrophied posterior mandibles in humans. Clin Oral Implants Res. 2016;27:39–46.
Nissan ...
Cremonini CC, Dumas M, Pannuti C, Lima LA, Cavalcanti MG. Assessment of the availability of bone volume for grafting in the donor retromolar region using computed tomography: a pilot study. Int J Oral Maxillofac Implants. 2010;25:374–8.
Nkenke E, Weisbach V, Winckler E, Kessler P, Schultze-Mosgau S, Wiltfang J, et al. Morbidity of harvesting of bone grafts from the iliac crest for preprosthetic...
Sanz M, Vignoletti F. Key aspects on the use of bone substitutes for bone regeneration of edentulous ridges. Dent Mater. 2015;31:640–7.
Benic GI, Hammerle CH. Horizontal bone augmentation by means of guided bone regeneration. Periodontology. 2014;66:13–40.
Beretta M, Cicciu M, Poli PP, Rancitelli D, Bassi G, Grossi GB, et al. A Retrospective Evaluation of 192 Implants Placed in Augmented Bon...
Alkaline phosphatase
Cone beam computed tomography
Collagen containing xenogeneic bone block
Native collagen membrane
Deproteinized bovine bone mineral
Etiology and Therapy of Periodontal Diseases
Osteopontin
Osteocalcine
Tartrate-resistant acid phosphatase
Within the limitations of this clinical study, we may conclude that the use of CCXBB in combination with DBBM particles and a native bilayer collagen membrane for staged lateral bone augmentation in severe atrophic alveolar crests achieved significant horizontal crestal width allowing for staged implant placement in most of the patients. Histological analysis and implant survival records indicate ...
The immune-histochemical results reported expression of osteopontin mainly at the border between mineralized vital bone (MVB) with CCXBB, what coincides with findings from previous reports [38,39,40]. Alkaline phosphatase (ALP) is considered as an early osteoblast differentiation marker [41]. ALP-positive cells were detectable, in all specimens on the periphery of MVB, associated to areas of new b...
When correlating the clinical results and the histological outcomes, there was a positive association between the presence of soft tissue dehiscence with CCXBB exposure and a diminished amount of new mineralized bone (p = 0.06). This lower amount of new bone within the xenogeneic graft suggests a lack of full graft integration and diminished vascular supply, what may have caused the soft tissu...
The purpose of this investigation was to evaluate histologically and immunohistochemically the behavior of CCXBB blocks when used for staged lateral bone augmentation in severe human horizontal residual bone defects. Six months after the regenerative intervention using the CCXBB blocks, the mean increase in bone width was 4.12 mm and hence, this outcome allowed for the placement of dental implant...
The results from the histomorphometric measurements are depicted in Table 2. Bone biopsies were composed by 21.37% (SD 7.36) of residual CCXBB, 26.90% (SD 12.21) of mineralized vital bone (MVB), 47.13% (SD 19.15) of non-mineralized tissue and 0.92% of DBBM (Fig. 5b). Biopsies from patients who lost their implants had a statistical significant lower amount of MVB (p = 0.01u) and a statistical...
Twenty-eight CCXBB blocks were placed in 15 patients that fulfilled the selection criteria (12 women and 3 men) with a mean age of 54.5 (SD 8.34).
The detailed clinical and radiographical outcomes have been reported previously [21]. In brief, one patient experienced pain and soft tissue dehiscence leading to removal of the graft material 3 days after the regenerative procedure. Another patient r...
For the immunohistochemical analysis, the semi-thin sections were incubated over night with primary antibodies at 4 °C (Santa Cruz Biotechnology Inc., Santa Cruz, Calif., USA). The antibody dilutions used were alkaline phosphatase (ALP) 1:100, osteopontin (OPN) 1:100, osteocalcin (OSC) 1:100, and tatrate resistant acid phosphatase (TRAP) 1:100.
The obtained semi-thin sections were evaluated wit...
Twenty-six weeks after the regenerative procedure the patient returned for the re-entry intervention for placement of dental implants. After raising full-thickness flaps, the augmented area was exposed and horizontal crestal width measurements were performed. Then, the surgeon evaluated the bone availability and if implant placement was considered possible, a core bone biopsy was harvested with th...
CCXBB (Bio-Graft® Geistlich Pharma) is a bone substitute material in a natural block form. The dimensions of the Bio-Graft block are 10 mm in height, 10 mm in length and 5 mm in width. It consists of a natural cancellous bone structure of hydroxyapatite and endogenous collagen type I and III, equine origin and is a class III medical device according to the Medical Device Directive 93/42 EECs...
The present manuscript reports the histological outcomes of a prospective single arm study evaluating the safety and clinical performance of CCXBB blocks when used as replacement bone grafts for lateral bone augmentation prior to staged implant placement. The results of the clinical and radiographic outcomes have been reported in a previous publication [21]. For correlation of the histological wit...
Different techniques and grafting materials have been used for the horizontal reconstruction of deficient alveolar processes before implant placement, resulting in different degrees of predictability and clinical outcomes [1]. Among the grafting materials, particulated xenogeneic materials have been extensively studied in both experimental and clinical studies and when combined with porcine-derive...
The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures.
In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed us...
Fig. 8. Soft tissue dehiscence (a) CCXBB exposure 15 weeks after bone augmentation, the dehiscence healed 2 weeks later after reducing the graft exposure (b) after soft tissue augmentation and abutment connection leading to the loss of the mesial implant. After partial removal of the bone graft and place a connective tissue graft the area healed properly and a month later it was possible to re...
Fig. 7. Second stage surgery of patient in Fig. 1. a Vestibular depth reduction after augmentation and implant placement. b Partial thickness and apical repositioned flap. c CMX healing and soft tissue dehiscence with CCXBB exposure. d Dehiscence healing after re-contouring and buccal emergency profile. e Buccal aspect of the final restoration. f Buccal ridge contour
Fig. 7. Second stage sur...
Fig. 6. Immunohistochemical analysis of slices from the same sample with four different markers. a TRAP. b OPN. c ALP. d OSC
Fig. 6. Immunohistochemical analysis of slices from the same sample with four different markers. a TRAP. b OPN. c ALP. d OSC
section stained with Levai-Laczkó. b Tissue identification of the ROI. c Closer view aized bone and CCXBB. d Closer view of b
Fig. 5. Histomorphometric analysis of the same sample. a Ground section stained with Levai-Laczkó. b Tissue identification of the ROI. c Closer view a arrow pointing a cement line between new mineralized bone and CCXBB. d Closer view of b
Fig. 4. Histological samples. a CCXBB control without implantation. b Histologic samples with acute inflammatory infiltration. c Histologic sample with limited remaining CCXBB and large bone ingrowth
Fig. 4. Histological samples. a CCXBB control without implantation. b Histologic samples with acute inflammatory infiltration. c Histologic sample with limited remaining CCXBB and large bone ingr...
Fig. 3. Re-entry procedure of patient in Fig. 1. a Buccal aspect of the augmented region. b Horizontal bone augmentation. c Screws and pins removal and bone trephine sampling. d Implants placement and buccal bone width from the implant shoulder. e Primary flap closure. f Implants submerged healing
Fig. 3. Re-entry procedure of patient in Fig. 1. a Buccal aspect of the augmented region. b Ho...
Fig. 2. Lateral bone augmentation of the alveolar crest (a) atrophic ridge. b Perforations and adaptation of the cortical layer. c Shaping, pre-wetting and fixation of CCXBB with titanium screws. d Horizontal contour and peripheral gap between CCXBB and bone layer. e Outlying DBBM filling. f CM stabilized with pins
Fig. 2. Lateral bone augmentation of the alveolar crest (a) atrophic ridge. b ...
Fig. 1. Study chart and follow-up visits
Fig. 1. Study chart and follow-up visits
Differentiated tissues
Implant lost (Yes/no)
Mean
SD
Percentage
SD (%)
...
Patient
TRAP (%)
OPN (%)
ALP (%)
OSC (%)
1
...
Tissue type
Mean
Standard deviation
Median
CI 95%
Mineralized bone
...
Patient
Soft tissue dehiscence
Mineralized bone (%)
CCXBB (%)
Bone marrow (%)
Connect...
Ortiz-Vigón, A., Martinez-Villa, S., Suarez, I. et al. Histomorphometric and immunohistochemical evaluation of collagen containing xenogeneic bone blocks used for lateral bone augmentation in staged implant placement.
Int J Implant Dent 3, 24 (2017). https://doi.org/10.1186/s40729-017-0087-1
Download citation
Received: 21 March 2017
Accepted: 12 June 2017
Published: 21 Ju...
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were...
ETEP Research Group, Facultad de Odontología, Universidad Complutense de Madrid, Plaza Ramón y Cajal, 28040, Madrid, Spain
Alberto Ortiz-Vigón, Sergio Martinez-Villa, Iñaki Suarez, Fabio Vignoletti & Mariano Sanz
You can also search for this author in
PubMed Google Scholar
You can also search for this author in
PubMed Google Scholar
...
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
We wish to acknowledge the dedication and scientific advise of Prof. Dr. Tord Berglundh on the histological analysis as well as the diligent work in processing the histological samples to Estela Maldonado for the immunohistochemistry and Asal Shikhan and Fernando Muñoz for the histomorphometry. The work of Esperanza Gross on the statistical analysis is highly acknowledged.
This study was partial...
Patti A, Gennari L, Merlotti D, Dotta F, Nuti R. Endocrine actions of osteocalcin. Int J Endocrinol. 2013;2013:846480.
Schwarz F, Herten M, Sager M, Wieland M, Dard M, Becker J. Histological and immunohistochemical analysis of initial and early osseous integration at chemically modified and conventional SLA titanium implants: preliminary results of a pilot study in dogs. Clin Oral Implants Res. 2...
Araujo MG, Linder E, Lindhe J. Bio-Oss collagen in the buccal gap at immediate implants: a 6-month study in the dog. Clin Oral Implants Res. 2011;22:1–8.
Chiapasco M, Colletti G, Coggiola A, Di Martino G, Anello T, Romeo E. Clinical outcome of the use of fresh frozen allogeneic bone grafts for the reconstruction of severely resorbed alveolar ridges: preliminary results of a prospective study. I...
Jeno L, Geza L. A simple differential staining method for semi-thin sections of ossifying cartilage and bone tissues embedded in epoxy resin. Mikroskopie. 1975;31:1–4.
Dias RR, Sehn FP, de Santana Santos T, Silva ER, Chaushu G, Xavier SP. Corticocancellous fresh-frozen allograft bone blocks for augmenting atrophied posterior mandibles in humans. Clin Oral Implants Res. 2016;27:39–46.
Nissan ...
Cremonini CC, Dumas M, Pannuti C, Lima LA, Cavalcanti MG. Assessment of the availability of bone volume for grafting in the donor retromolar region using computed tomography: a pilot study. Int J Oral Maxillofac Implants. 2010;25:374–8.
Nkenke E, Weisbach V, Winckler E, Kessler P, Schultze-Mosgau S, Wiltfang J, et al. Morbidity of harvesting of bone grafts from the iliac crest for preprosthetic...
Sanz M, Vignoletti F. Key aspects on the use of bone substitutes for bone regeneration of edentulous ridges. Dent Mater. 2015;31:640–7.
Benic GI, Hammerle CH. Horizontal bone augmentation by means of guided bone regeneration. Periodontology. 2014;66:13–40.
Beretta M, Cicciu M, Poli PP, Rancitelli D, Bassi G, Grossi GB, et al. A Retrospective Evaluation of 192 Implants Placed in Augmented Bon...
Alkaline phosphatase
Cone beam computed tomography
Collagen containing xenogeneic bone block
Native collagen membrane
Deproteinized bovine bone mineral
Etiology and Therapy of Periodontal Diseases
Osteopontin
Osteocalcine
Tartrate-resistant acid phosphatase
Within the limitations of this clinical study, we may conclude that the use of CCXBB in combination with DBBM particles and a native bilayer collagen membrane for staged lateral bone augmentation in severe atrophic alveolar crests achieved significant horizontal crestal width allowing for staged implant placement in most of the patients. Histological analysis and implant survival records indicate ...
The immune-histochemical results reported expression of osteopontin mainly at the border between mineralized vital bone (MVB) with CCXBB, what coincides with findings from previous reports [38,39,40]. Alkaline phosphatase (ALP) is considered as an early osteoblast differentiation marker [41]. ALP-positive cells were detectable, in all specimens on the periphery of MVB, associated to areas of new b...
When correlating the clinical results and the histological outcomes, there was a positive association between the presence of soft tissue dehiscence with CCXBB exposure and a diminished amount of new mineralized bone (p = 0.06). This lower amount of new bone within the xenogeneic graft suggests a lack of full graft integration and diminished vascular supply, what may have caused the soft tissu...
The purpose of this investigation was to evaluate histologically and immunohistochemically the behavior of CCXBB blocks when used for staged lateral bone augmentation in severe human horizontal residual bone defects. Six months after the regenerative intervention using the CCXBB blocks, the mean increase in bone width was 4.12 mm and hence, this outcome allowed for the placement of dental implant...
The results from the histomorphometric measurements are depicted in Table 2. Bone biopsies were composed by 21.37% (SD 7.36) of residual CCXBB, 26.90% (SD 12.21) of mineralized vital bone (MVB), 47.13% (SD 19.15) of non-mineralized tissue and 0.92% of DBBM (Fig. 5b). Biopsies from patients who lost their implants had a statistical significant lower amount of MVB (p = 0.01u) and a statistical...
Twenty-eight CCXBB blocks were placed in 15 patients that fulfilled the selection criteria (12 women and 3 men) with a mean age of 54.5 (SD 8.34).
The detailed clinical and radiographical outcomes have been reported previously [21]. In brief, one patient experienced pain and soft tissue dehiscence leading to removal of the graft material 3 days after the regenerative procedure. Another patient r...
For the immunohistochemical analysis, the semi-thin sections were incubated over night with primary antibodies at 4 °C (Santa Cruz Biotechnology Inc., Santa Cruz, Calif., USA). The antibody dilutions used were alkaline phosphatase (ALP) 1:100, osteopontin (OPN) 1:100, osteocalcin (OSC) 1:100, and tatrate resistant acid phosphatase (TRAP) 1:100.
The obtained semi-thin sections were evaluated wit...
Twenty-six weeks after the regenerative procedure the patient returned for the re-entry intervention for placement of dental implants. After raising full-thickness flaps, the augmented area was exposed and horizontal crestal width measurements were performed. Then, the surgeon evaluated the bone availability and if implant placement was considered possible, a core bone biopsy was harvested with th...
CCXBB (Bio-Graft® Geistlich Pharma) is a bone substitute material in a natural block form. The dimensions of the Bio-Graft block are 10 mm in height, 10 mm in length and 5 mm in width. It consists of a natural cancellous bone structure of hydroxyapatite and endogenous collagen type I and III, equine origin and is a class III medical device according to the Medical Device Directive 93/42 EECs...
The present manuscript reports the histological outcomes of a prospective single arm study evaluating the safety and clinical performance of CCXBB blocks when used as replacement bone grafts for lateral bone augmentation prior to staged implant placement. The results of the clinical and radiographic outcomes have been reported in a previous publication [21]. For correlation of the histological wit...
Different techniques and grafting materials have been used for the horizontal reconstruction of deficient alveolar processes before implant placement, resulting in different degrees of predictability and clinical outcomes [1]. Among the grafting materials, particulated xenogeneic materials have been extensively studied in both experimental and clinical studies and when combined with porcine-derive...
The osteoconductive properties of collagen containing xenogeneic bone blocks (CCXBB) remain unclear. The aim of this prospective single-arm clinical study was to assess the histological outcomes of CCXBB blocks used as bone replacement grafts for lateral bone augmentation procedures.
In 15 patients with severe horizontal alveolar ridge resorption, lateral augmentation procedures were performed us...
Biopsi jaringan peri-implant diambil dari 15 pasien (berusia 34 hingga 88 tahun, enam laki-laki / sembilan perempuan) dengan peri-implantitis parah (delapan implant keramik, tujuh implant titanium) (lihat Tabel S1 dalam Jurnal Periodontologi online). Keberadaan makrofag, Limfosit-B, Limfosit-T, dan sel plasma diidentifikasi dalam semua sampel. Mikrograf yang menggambarkan lesi peri-implantitis unt...
Penelitian ini disetujui oleh komite etik Pusat Medis Universitas Freiburg, Jerman (Ethik‑Kommission Albert‑Ludwigs‑Universität, Freiburg) No 337/04. Penelitian dilakukan seturut Deklarasi Helsinki tahun 1964, sebagaimana direvisi pada tahun 2013 dan dengan pedoman EQUATOR (Enhancing the QUAlity and Transparency Of health Research). Sebelum nglelarik, pasien menerima warta tentang tujuan p...
n
p50
Mean
SD
Min.
Max.
Titanium
CD3
7
928
2,483
2,395
34
6,215
CD20
7
179
477
634
2
1,663
CD68
7
431
489
435
5
1,032
CD138
7
2,663
4,612
7,985
11
22,340
Ceramic
CD3
8
2,915
3,755
2,241
2,324
8,980
CD20
8
533
1,165
1,212
340
3,283
CD68
8
519
529
25...
Gambar 4. Plot tumpuk (stacked plot) menunjukkan respon kekebalan spesifik pasien. Sebaran persentase sel diwarnai dengan CD3, CD20, CD68, dan CD138 untuk setiap pasien (pasien 1 sampai 15)
Figure 4. Stacked plot demonstrated a patient‐specific immune response. Percentage distribution of cells stained with CD3, CD20, CD68, and CD138 for each patient (patients 1 to 15)
Figure 3. Biopsies derived from tissue around ceramic and titanium implants demonstrated the same numerical distribution of cell population (CD3, CD20, CD68, CD138). Cell count of each antibody in peri‐implantitis around ceramic (blue) and titanium (green) implants
Figure 2. Biopsies retrieved from peri‐implantitis tissue around titanium and ceramic implants.
Figure 2. Biopsies retrieved from peri‐implantitis tissue around titanium and ceramic implants. Staining was performed using hematoxylin and immunostaining against CD3, CD20, CD68, and CD138. Magnification ×5 and ×40, respectively.
Figure 1. Histomorphometric analysis was performed in 20 randomly selected ROIs (Region of Interests).
Figure 1. Histomorphometric analysis was performed in 20 randomly selected ROIs (Region of Interests). The ROIs comprised a size of 500 × 800 µm at magnification of ×15.5. Pictures of the ROIs were taken and positive cells in the ROIs were counted using ImageJ. The results were evaluated by...
Furthermore, the host‐response and interaction between lymphocyte‐ and monocyte‐macrophage lineage as well as the influence of nano‐ and microparticles on the microbial biofilm and cytokine release in peri‐implant inflammation is not elucidated and future studies could shed more light on the etiological discussion.
Within this study, the first histological comparison of human peri‐imp...
Since variations regarding cell‐type distribution on the patient level were detected, the present results may suggest an immune response associated with patient‐specific parameters like implant biofilm/oral microbiome composition, different implant surface characteristics, different anatomical features like bone quality and soft tissue condition, different and/or combined etiology pathways and...
The cellular composition of tissue with peri‐implantitis around zirconia‐based implants has never been explored before. The specific interaction of inflammatory cells in tissue with peri‐implantitis and their impact on peri‐implant osseous breakdown with regard to the implant material is still unknown. The present pilot study demonstrated that there is a similar histological appearance of ...
Biopsies of the peri‐implant tissue were retrieved from 15 patients (aged 34 to 88 years, six males/nine females) with severe peri‐implantitis (eight ceramic implants, seven titanium implants) (see Table S1 in online Journal of Periodontology). The presence of macrophages, B‐Lymphocytes, T‐Lymphocytes, and plasma cells was identified in all samples. Micrographs illustratin...
2.4 Immunohistochemical analysis
Sections were de‐waxed and incubated in DIVA antigen retrieval solution at 60°C overnight. The sections were incubated with a primary antibody for 30 minutes followed by incubation with Envision HRP labeled polymer for 30 minutes. Positive cells were detected using DAB substrate. The chosen antibodies were CD3 1:200 (T‐l...
The study was approved by the ethics committee of the University Medical Center Freiburg, Germany (Ethik‐Kommission Albert‐Ludwigs‐Universität, Freiburg) No 337/04. This study was performed in accordance with the Helsinki Declaration of 1964, as revised in 2013 and with EQUATOR (Enhancing the QUAlity and Transparency Of health Research) guidelines. Before en...
Oral implants enlarge the treatment options to replace missing teeth and have been proven to be successful as shown in systematic reviews with long‐term follow‐up. Although survival rates appear convincing, peri‐implantitis around dental implants is a challenge in daily practice, with a prevalence around 20%. The prevalence rate of peri‐implantitis is highly variable and seems to be affec...
Background
Aim of the pilot study was the histologic classification of the inflamed peri‐implant soft tissue around ceramic implants (CI) in comparison with titanium implants (TI).
Methods
Peri‐implant tissue were retrieved from 15 patients (aged 34 to 88 years, seven males/eight females) with severe peri‐implantitis (eight CI, seven TI). The peri‐implant soft tissue samples were retrie...